Strain Fitness in Escherichia coli BW25113 around b0962

Experiment: D-Serine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb0960 and yccF are separated by 18 nucleotidesyccF and helD are separated by 122 nucleotideshelD and mgsA are separated by 31 nucleotidesmgsA and yccT are separated by 95 nucleotides b0960: b0960 - orf, hypothetical protein (VIMSS), at 1,020,953 to 1,023,106 b0960 b0961: yccF - conserved inner membrane protein (NCBI), at 1,023,125 to 1,023,571 yccF b0962: helD - DNA helicase IV (NCBI), at 1,023,694 to 1,025,748 helD b0963: mgsA - methylglyoxal synthase (VIMSS), at 1,025,780 to 1,026,238 mgsA b0964: yccT - hypothetical protein (NCBI), at 1,026,334 to 1,026,996 yccT Position (kb) 1023 1024 1025 1026Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1022.703 kb on + strand, within b0960at 1022.713 kb on - strand, within b0960at 1022.810 kb on + strand, within b0960at 1022.819 kb on + strand, within b0960at 1022.852 kb on - strand, within b0960at 1022.884 kb on - strand, within b0960at 1022.884 kb on - strand, within b0960at 1022.886 kb on + strand, within b0960at 1023.008 kb on + strandat 1023.160 kb on + strandat 1023.160 kb on + strandat 1023.170 kb on - strand, within yccFat 1023.179 kb on - strand, within yccFat 1023.400 kb on + strand, within yccFat 1023.423 kb on + strand, within yccFat 1023.423 kb on + strand, within yccFat 1023.488 kb on + strand, within yccFat 1023.512 kb on + strand, within yccFat 1023.525 kb on - strand, within yccFat 1023.525 kb on - strand, within yccFat 1023.588 kb on - strandat 1023.689 kb on - strandat 1023.689 kb on - strandat 1023.698 kb on - strandat 1023.770 kb on + strandat 1023.770 kb on + strandat 1023.867 kb on - strandat 1023.867 kb on - strandat 1023.876 kb on + strandat 1023.937 kb on - strand, within helDat 1024.110 kb on + strand, within helDat 1024.181 kb on - strand, within helDat 1024.387 kb on - strand, within helDat 1024.483 kb on - strand, within helDat 1024.483 kb on - strand, within helDat 1024.499 kb on + strand, within helDat 1024.801 kb on - strand, within helDat 1024.801 kb on - strand, within helDat 1024.971 kb on + strand, within helDat 1024.988 kb on + strand, within helDat 1024.988 kb on + strand, within helDat 1025.201 kb on - strand, within helDat 1025.201 kb on - strand, within helDat 1025.213 kb on + strand, within helDat 1025.213 kb on + strand, within helDat 1025.399 kb on + strand, within helDat 1025.424 kb on + strand, within helDat 1025.424 kb on + strand, within helDat 1025.661 kb on + strandat 1025.662 kb on + strandat 1025.669 kb on - strandat 1025.772 kb on + strandat 1025.827 kb on - strand, within mgsAat 1025.893 kb on - strand, within mgsAat 1025.921 kb on + strand, within mgsAat 1025.943 kb on - strand, within mgsAat 1025.943 kb on - strand, within mgsAat 1026.042 kb on - strand, within mgsAat 1026.043 kb on - strandat 1026.054 kb on + strand, within mgsAat 1026.115 kb on + strand, within mgsAat 1026.116 kb on - strand, within mgsAat 1026.119 kb on + strand, within mgsAat 1026.123 kb on - strand, within mgsAat 1026.153 kb on + strand, within mgsAat 1026.153 kb on + strand, within mgsAat 1026.295 kb on + strandat 1026.403 kb on - strand, within yccTat 1026.403 kb on - strand, within yccTat 1026.627 kb on + strand, within yccTat 1026.627 kb on + strandat 1026.685 kb on + strand, within yccTat 1026.740 kb on + strand, within yccTat 1026.740 kb on + strand, within yccT

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Serine (C)
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1,022,703 + b0960 b0960 0.81 +2.6
1,022,713 - b0960 b0960 0.82 +2.5
1,022,810 + b0960 b0960 0.86 +1.6
1,022,819 + b0960 b0960 0.87 +2.9
1,022,852 - b0960 b0960 0.88 +1.4
1,022,884 - b0960 b0960 0.90 +3.0
1,022,884 - b0960 b0960 0.90 +2.9
1,022,886 + b0960 b0960 0.90 +2.8
1,023,008 + +2.9
1,023,160 + +1.5
1,023,160 + +0.6
1,023,170 - yccF b0961 0.10 -0.5
1,023,179 - yccF b0961 0.12 -1.9
1,023,400 + yccF b0961 0.62 +1.4
1,023,423 + yccF b0961 0.67 +1.0
1,023,423 + yccF b0961 0.67 +1.2
1,023,488 + yccF b0961 0.81 +2.0
1,023,512 + yccF b0961 0.87 +2.1
1,023,525 - yccF b0961 0.89 +0.3
1,023,525 - yccF b0961 0.89 +0.2
1,023,588 - -0.9
1,023,689 - +0.8
1,023,689 - -0.4
1,023,698 - -0.8
1,023,770 + -0.0
1,023,770 + +0.1
1,023,867 - -0.3
1,023,867 - +0.2
1,023,876 + -0.7
1,023,937 - helD b0962 0.12 +0.1
1,024,110 + helD b0962 0.20 +0.7
1,024,181 - helD b0962 0.24 -0.0
1,024,387 - helD b0962 0.34 +0.6
1,024,483 - helD b0962 0.38 -0.3
1,024,483 - helD b0962 0.38 +0.2
1,024,499 + helD b0962 0.39 +0.7
1,024,801 - helD b0962 0.54 -0.0
1,024,801 - helD b0962 0.54 -0.7
1,024,971 + helD b0962 0.62 -0.6
1,024,988 + helD b0962 0.63 +2.0
1,024,988 + helD b0962 0.63 -0.1
1,025,201 - helD b0962 0.73 -0.1
1,025,201 - helD b0962 0.73 +0.0
1,025,213 + helD b0962 0.74 -0.3
1,025,213 + helD b0962 0.74 -0.4
1,025,399 + helD b0962 0.83 -0.6
1,025,424 + helD b0962 0.84 -0.1
1,025,424 + helD b0962 0.84 -1.6
1,025,661 + -1.0
1,025,662 + -0.4
1,025,669 - -0.1
1,025,772 + -0.1
1,025,827 - mgsA b0963 0.10 +0.3
1,025,893 - mgsA b0963 0.25 +0.4
1,025,921 + mgsA b0963 0.31 -0.1
1,025,943 - mgsA b0963 0.36 -1.9
1,025,943 - mgsA b0963 0.36 +0.1
1,026,042 - mgsA b0963 0.57 -0.3
1,026,043 - +1.6
1,026,054 + mgsA b0963 0.60 -0.4
1,026,115 + mgsA b0963 0.73 -0.5
1,026,116 - mgsA b0963 0.73 -0.3
1,026,119 + mgsA b0963 0.74 -0.2
1,026,123 - mgsA b0963 0.75 +0.1
1,026,153 + mgsA b0963 0.81 +0.9
1,026,153 + mgsA b0963 0.81 +1.1
1,026,295 + +0.4
1,026,403 - yccT b0964 0.10 +0.0
1,026,403 - yccT b0964 0.10 +0.4
1,026,627 + yccT b0964 0.44 -1.1
1,026,627 + +1.4
1,026,685 + yccT b0964 0.53 -0.1
1,026,740 + yccT b0964 0.61 -0.4
1,026,740 + yccT b0964 0.61 -3.3

Or see this region's nucleotide sequence