Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4474 and EX28DRAFT_4475 are separated by 329 nucleotides EX28DRAFT_4475 and EX28DRAFT_4476 are separated by 95 nucleotides EX28DRAFT_4476 and EX28DRAFT_4477 are separated by 135 nucleotides EX28DRAFT_4477 and EX28DRAFT_4478 are separated by 35 nucleotides
EX28DRAFT_4474: EX28DRAFT_4474 - hydroxymethylbilane synthase (EC 2.5.1.61), at 38,802 to 39,764
_4474
EX28DRAFT_4475: EX28DRAFT_4475 - Adenylate cyclase, at 40,094 to 42,637
_4475
EX28DRAFT_4476: EX28DRAFT_4476 - iron donor protein CyaY, at 42,733 to 43,053
_4476
EX28DRAFT_4477: EX28DRAFT_4477 - Predicted small periplasmic lipoprotein, at 43,189 to 43,392
_4477
EX28DRAFT_4478: EX28DRAFT_4478 - diaminopimelate epimerase (EC 5.1.1.7), at 43,428 to 44,252
_4478
Position (kb) 40
41
42
43 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 39.779 kb on - strand at 39.808 kb on - strand at 39.861 kb on + strand at 39.862 kb on - strand at 39.880 kb on - strand at 39.892 kb on + strand at 39.892 kb on + strand at 39.900 kb on + strand at 39.900 kb on + strand at 39.901 kb on - strand at 39.901 kb on - strand at 39.901 kb on - strand at 39.901 kb on - strand at 39.901 kb on - strand at 39.901 kb on - strand at 39.905 kb on - strand at 39.975 kb on + strand at 39.981 kb on + strand at 40.013 kb on + strand at 41.447 kb on - strand, within EX28DRAFT_4475 at 41.450 kb on + strand, within EX28DRAFT_4475 at 41.724 kb on - strand, within EX28DRAFT_4475 at 41.772 kb on - strand, within EX28DRAFT_4475 at 42.274 kb on + strand, within EX28DRAFT_4475 at 42.316 kb on + strand, within EX28DRAFT_4475 at 42.365 kb on + strand, within EX28DRAFT_4475 at 42.366 kb on - strand, within EX28DRAFT_4475 at 42.398 kb on + strand at 42.399 kb on - strand at 42.494 kb on - strand at 42.545 kb on - strand at 42.679 kb on + strand at 42.735 kb on - strand at 43.087 kb on - strand at 43.099 kb on - strand at 43.173 kb on + strand at 43.174 kb on - strand at 43.185 kb on + strand at 43.267 kb on + strand, within EX28DRAFT_4477 at 43.268 kb on - strand, within EX28DRAFT_4477 at 43.268 kb on - strand, within EX28DRAFT_4477 at 43.268 kb on - strand, within EX28DRAFT_4477 at 43.344 kb on + strand, within EX28DRAFT_4477 at 43.344 kb on + strand, within EX28DRAFT_4477 at 43.347 kb on + strand, within EX28DRAFT_4477 at 43.439 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 39,779 - +0.4 39,808 - +0.8 39,861 + -0.0 39,862 - -0.1 39,880 - +0.0 39,892 + -0.1 39,892 + -0.0 39,900 + +0.2 39,900 + -0.7 39,901 - -0.7 39,901 - +0.2 39,901 - +0.2 39,901 - +0.0 39,901 - -0.0 39,901 - -0.2 39,905 - +0.1 39,975 + -0.6 39,981 + +0.6 40,013 + +0.2 41,447 - EX28DRAFT_4475 0.53 -1.2 41,450 + EX28DRAFT_4475 0.53 -1.6 41,724 - EX28DRAFT_4475 0.64 -2.5 41,772 - EX28DRAFT_4475 0.66 -1.2 42,274 + EX28DRAFT_4475 0.86 -2.4 42,316 + EX28DRAFT_4475 0.87 -3.7 42,365 + EX28DRAFT_4475 0.89 -0.9 42,366 - EX28DRAFT_4475 0.89 -1.3 42,398 + -0.3 42,399 - -1.7 42,494 - -2.2 42,545 - +0.7 42,679 + -0.2 42,735 - -0.1 43,087 - +0.3 43,099 - -0.6 43,173 + -0.1 43,174 - +0.3 43,185 + +1.7 43,267 + EX28DRAFT_4477 0.38 +0.8 43,268 - EX28DRAFT_4477 0.39 -1.3 43,268 - EX28DRAFT_4477 0.39 -1.2 43,268 - EX28DRAFT_4477 0.39 +0.8 43,344 + EX28DRAFT_4477 0.76 +0.2 43,344 + EX28DRAFT_4477 0.76 +1.0 43,347 + EX28DRAFT_4477 0.77 -0.5 43,439 + +0.2
Or see this region's nucleotide sequence