Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4120 and EX28DRAFT_4121 are separated by 188 nucleotides EX28DRAFT_4121 and EX28DRAFT_4122 are separated by 47 nucleotides EX28DRAFT_4122 and EX28DRAFT_4123 are separated by 16 nucleotides
EX28DRAFT_4120: EX28DRAFT_4120 - Glycerol-3-phosphate dehydrogenase, at 95,624 to 97,132
_4120
EX28DRAFT_4121: EX28DRAFT_4121 - Rhodanese-related sulfurtransferase, at 97,321 to 97,653
_4121
EX28DRAFT_4122: EX28DRAFT_4122 - rhomboid family protease GlpG, at 97,701 to 98,531
_4122
EX28DRAFT_4123: EX28DRAFT_4123 - transcriptional regulator, DeoR family, at 98,548 to 99,306
_4123
Position (kb) 97
98 Strain fitness (log2 ratio) -2
-1
0
1
2 at 96.814 kb on + strand, within EX28DRAFT_4120 at 96.815 kb on - strand, within EX28DRAFT_4120 at 96.815 kb on - strand, within EX28DRAFT_4120 at 96.957 kb on + strand, within EX28DRAFT_4120 at 97.227 kb on + strand at 97.246 kb on + strand at 97.247 kb on - strand at 97.247 kb on - strand at 97.273 kb on + strand at 97.273 kb on + strand at 97.309 kb on - strand at 97.339 kb on - strand at 97.341 kb on + strand at 97.346 kb on + strand at 97.346 kb on + strand at 97.347 kb on - strand at 97.347 kb on - strand at 97.549 kb on + strand, within EX28DRAFT_4121 at 97.580 kb on - strand, within EX28DRAFT_4121 at 97.580 kb on - strand, within EX28DRAFT_4121 at 97.580 kb on - strand, within EX28DRAFT_4121 at 97.636 kb on + strand at 97.662 kb on + strand at 97.662 kb on + strand at 97.662 kb on + strand at 97.662 kb on + strand at 97.662 kb on + strand at 97.663 kb on - strand at 97.663 kb on - strand at 97.721 kb on + strand at 97.721 kb on + strand at 97.722 kb on - strand at 97.757 kb on + strand at 97.796 kb on + strand, within EX28DRAFT_4122 at 97.796 kb on + strand, within EX28DRAFT_4122 at 98.027 kb on + strand, within EX28DRAFT_4122 at 98.030 kb on + strand, within EX28DRAFT_4122 at 98.179 kb on - strand, within EX28DRAFT_4122 at 98.313 kb on + strand, within EX28DRAFT_4122 at 98.313 kb on + strand, within EX28DRAFT_4122 at 98.313 kb on + strand, within EX28DRAFT_4122 at 98.314 kb on - strand, within EX28DRAFT_4122 at 98.314 kb on - strand, within EX28DRAFT_4122 at 98.328 kb on + strand, within EX28DRAFT_4122 at 98.328 kb on + strand, within EX28DRAFT_4122 at 98.329 kb on - strand, within EX28DRAFT_4122 at 98.370 kb on + strand, within EX28DRAFT_4122 at 98.371 kb on - strand, within EX28DRAFT_4122 at 98.387 kb on - strand, within EX28DRAFT_4122 at 98.387 kb on - strand, within EX28DRAFT_4122 at 98.444 kb on + strand, within EX28DRAFT_4122 at 98.529 kb on + strand at 98.529 kb on + strand at 98.602 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 96,814 + EX28DRAFT_4120 0.79 -0.3 96,815 - EX28DRAFT_4120 0.79 +0.3 96,815 - EX28DRAFT_4120 0.79 -0.8 96,957 + EX28DRAFT_4120 0.88 -0.9 97,227 + -0.1 97,246 + -0.0 97,247 - -2.6 97,247 - +0.7 97,273 + -1.9 97,273 + +0.2 97,309 - -0.4 97,339 - -1.4 97,341 + -0.3 97,346 + -1.0 97,346 + -0.4 97,347 - -0.3 97,347 - -0.6 97,549 + EX28DRAFT_4121 0.68 -0.8 97,580 - EX28DRAFT_4121 0.78 -0.3 97,580 - EX28DRAFT_4121 0.78 -1.0 97,580 - EX28DRAFT_4121 0.78 -0.5 97,636 + +0.4 97,662 + +0.1 97,662 + -0.3 97,662 + +1.4 97,662 + +0.9 97,662 + +0.5 97,663 - +0.2 97,663 - +0.3 97,721 + -1.2 97,721 + +0.0 97,722 - +0.7 97,757 + +0.6 97,796 + EX28DRAFT_4122 0.11 -0.6 97,796 + EX28DRAFT_4122 0.11 +0.3 98,027 + EX28DRAFT_4122 0.39 -0.0 98,030 + EX28DRAFT_4122 0.40 +0.3 98,179 - EX28DRAFT_4122 0.58 +0.1 98,313 + EX28DRAFT_4122 0.74 +0.3 98,313 + EX28DRAFT_4122 0.74 +0.4 98,313 + EX28DRAFT_4122 0.74 -0.4 98,314 - EX28DRAFT_4122 0.74 +1.4 98,314 - EX28DRAFT_4122 0.74 +2.0 98,328 + EX28DRAFT_4122 0.75 +0.2 98,328 + EX28DRAFT_4122 0.75 -0.3 98,329 - EX28DRAFT_4122 0.76 +0.1 98,370 + EX28DRAFT_4122 0.81 -0.6 98,371 - EX28DRAFT_4122 0.81 -0.5 98,387 - EX28DRAFT_4122 0.83 -0.5 98,387 - EX28DRAFT_4122 0.83 +0.1 98,444 + EX28DRAFT_4122 0.89 +0.6 98,529 + -0.3 98,529 + -1.2 98,602 + -0.9
Or see this region's nucleotide sequence