Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4041 and EX28DRAFT_4042 are separated by 175 nucleotides EX28DRAFT_4042 and EX28DRAFT_4043 are separated by 11 nucleotides EX28DRAFT_4043 and EX28DRAFT_4044 overlap by 4 nucleotides
EX28DRAFT_4041: EX28DRAFT_4041 - cellulose biosynthesis protein BcsE, at 5,322 to 6,884
_4041
EX28DRAFT_4042: EX28DRAFT_4042 - Protein of unknown function (DUF2629), at 7,060 to 7,248
_4042
EX28DRAFT_4043: EX28DRAFT_4043 - cellulose synthase operon protein YhjQ, at 7,260 to 8,012
_4043
EX28DRAFT_4044: EX28DRAFT_4044 - cellulose synthase catalytic subunit (UDP-forming), at 8,009 to 10,624
_4044
Position (kb)
7
8 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6.192 kb on + strand, within EX28DRAFT_4041 at 6.203 kb on + strand, within EX28DRAFT_4041 at 6.204 kb on - strand, within EX28DRAFT_4041 at 6.284 kb on + strand, within EX28DRAFT_4041 at 6.355 kb on + strand, within EX28DRAFT_4041 at 6.355 kb on + strand, within EX28DRAFT_4041 at 6.355 kb on + strand, within EX28DRAFT_4041 at 6.414 kb on + strand, within EX28DRAFT_4041 at 6.415 kb on - strand, within EX28DRAFT_4041 at 6.437 kb on + strand, within EX28DRAFT_4041 at 6.491 kb on + strand, within EX28DRAFT_4041 at 6.517 kb on - strand, within EX28DRAFT_4041 at 6.571 kb on - strand, within EX28DRAFT_4041 at 6.626 kb on + strand, within EX28DRAFT_4041 at 6.693 kb on - strand, within EX28DRAFT_4041 at 6.791 kb on + strand at 6.791 kb on + strand at 6.803 kb on + strand at 6.804 kb on - strand at 6.866 kb on + strand at 6.908 kb on + strand at 6.909 kb on - strand at 6.909 kb on - strand at 6.915 kb on - strand at 6.938 kb on + strand at 6.945 kb on - strand at 6.951 kb on - strand at 6.979 kb on - strand at 6.979 kb on - strand at 6.985 kb on + strand at 6.986 kb on - strand at 6.986 kb on - strand at 6.986 kb on - strand at 7.065 kb on + strand at 7.166 kb on - strand, within EX28DRAFT_4042 at 7.246 kb on + strand at 7.246 kb on + strand at 7.345 kb on + strand, within EX28DRAFT_4043 at 7.346 kb on - strand, within EX28DRAFT_4043 at 7.346 kb on - strand, within EX28DRAFT_4043 at 7.394 kb on + strand, within EX28DRAFT_4043 at 7.394 kb on + strand, within EX28DRAFT_4043 at 7.395 kb on - strand, within EX28DRAFT_4043 at 7.395 kb on - strand, within EX28DRAFT_4043 at 7.395 kb on - strand, within EX28DRAFT_4043 at 7.454 kb on + strand, within EX28DRAFT_4043 at 7.483 kb on - strand, within EX28DRAFT_4043 at 7.484 kb on + strand, within EX28DRAFT_4043 at 7.484 kb on + strand, within EX28DRAFT_4043 at 7.484 kb on + strand, within EX28DRAFT_4043 at 7.611 kb on + strand, within EX28DRAFT_4043 at 7.611 kb on + strand, within EX28DRAFT_4043 at 7.612 kb on - strand, within EX28DRAFT_4043 at 7.685 kb on + strand, within EX28DRAFT_4043 at 7.687 kb on + strand, within EX28DRAFT_4043 at 7.688 kb on - strand, within EX28DRAFT_4043 at 7.688 kb on - strand, within EX28DRAFT_4043 at 7.688 kb on - strand, within EX28DRAFT_4043 at 7.688 kb on - strand, within EX28DRAFT_4043 at 8.008 kb on + strand at 8.008 kb on + strand at 8.008 kb on + strand at 8.009 kb on - strand at 8.182 kb on - strand at 8.182 kb on - strand at 8.202 kb on + strand at 8.202 kb on + strand at 8.202 kb on + strand at 8.202 kb on + strand at 8.203 kb on - strand at 8.203 kb on - strand at 8.203 kb on - strand at 8.203 kb on - strand at 8.203 kb on - strand at 8.216 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 6,192 + EX28DRAFT_4041 0.56 +0.4 6,203 + EX28DRAFT_4041 0.56 +0.6 6,204 - EX28DRAFT_4041 0.56 +0.1 6,284 + EX28DRAFT_4041 0.62 +0.4 6,355 + EX28DRAFT_4041 0.66 -0.3 6,355 + EX28DRAFT_4041 0.66 -0.3 6,355 + EX28DRAFT_4041 0.66 -0.1 6,414 + EX28DRAFT_4041 0.70 -1.7 6,415 - EX28DRAFT_4041 0.70 +0.6 6,437 + EX28DRAFT_4041 0.71 -0.3 6,491 + EX28DRAFT_4041 0.75 -0.1 6,517 - EX28DRAFT_4041 0.76 -0.8 6,571 - EX28DRAFT_4041 0.80 -0.2 6,626 + EX28DRAFT_4041 0.83 -0.4 6,693 - EX28DRAFT_4041 0.88 +1.0 6,791 + +0.4 6,791 + +0.5 6,803 + +0.2 6,804 - +0.4 6,866 + +0.7 6,908 + -0.9 6,909 - +1.4 6,909 - -2.3 6,915 - -0.2 6,938 + -3.4 6,945 - +0.2 6,951 - +0.3 6,979 - -0.8 6,979 - -0.1 6,985 + -0.1 6,986 - +0.3 6,986 - -0.6 6,986 - -0.4 7,065 + -0.6 7,166 - EX28DRAFT_4042 0.56 -1.2 7,246 + +0.1 7,246 + +0.0 7,345 + EX28DRAFT_4043 0.11 -0.4 7,346 - EX28DRAFT_4043 0.11 +0.4 7,346 - EX28DRAFT_4043 0.11 -0.3 7,394 + EX28DRAFT_4043 0.18 -0.2 7,394 + EX28DRAFT_4043 0.18 +0.5 7,395 - EX28DRAFT_4043 0.18 +0.6 7,395 - EX28DRAFT_4043 0.18 +1.6 7,395 - EX28DRAFT_4043 0.18 +0.4 7,454 + EX28DRAFT_4043 0.26 -0.7 7,483 - EX28DRAFT_4043 0.30 +0.1 7,484 + EX28DRAFT_4043 0.30 -0.0 7,484 + EX28DRAFT_4043 0.30 +1.0 7,484 + EX28DRAFT_4043 0.30 +0.4 7,611 + EX28DRAFT_4043 0.47 -0.1 7,611 + EX28DRAFT_4043 0.47 +0.2 7,612 - EX28DRAFT_4043 0.47 +0.8 7,685 + EX28DRAFT_4043 0.56 +0.3 7,687 + EX28DRAFT_4043 0.57 -0.6 7,688 - EX28DRAFT_4043 0.57 +1.9 7,688 - EX28DRAFT_4043 0.57 -0.3 7,688 - EX28DRAFT_4043 0.57 +0.7 7,688 - EX28DRAFT_4043 0.57 +0.2 8,008 + +0.7 8,008 + +0.0 8,008 + -0.4 8,009 - -0.1 8,182 - -0.4 8,182 - -0.2 8,202 + -0.1 8,202 + +0.7 8,202 + -0.2 8,202 + +0.2 8,203 - +0.5 8,203 - +0.1 8,203 - +0.4 8,203 - -0.1 8,203 - -0.3 8,216 - -0.2
Or see this region's nucleotide sequence