Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3965 and EX28DRAFT_3966 are separated by 17 nucleotides EX28DRAFT_3966 and EX28DRAFT_3967 are separated by 82 nucleotides EX28DRAFT_3967 and EX28DRAFT_3968 overlap by 11 nucleotides EX28DRAFT_3968 and EX28DRAFT_3969 are separated by 92 nucleotides
EX28DRAFT_3965: EX28DRAFT_3965 - gluconate transporter, at 173,198 to 174,535
_3965
EX28DRAFT_3966: EX28DRAFT_3966 - D-serine ammonia-lyase (EC 4.3.1.18), at 174,553 to 175,839
_3966
EX28DRAFT_3967: EX28DRAFT_3967 - Domain of unknown function (DUF202), at 175,922 to 176,269
_3967
EX28DRAFT_3968: EX28DRAFT_3968 - Predicted membrane protein, at 176,259 to 176,606
_3968
EX28DRAFT_3969: EX28DRAFT_3969 - Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases, at 176,699 to 178,021
_3969
Position (kb) 174
175
176 Strain fitness (log2 ratio) -2
-1
0
1
2 at 173.704 kb on + strand, within EX28DRAFT_3965 at 173.704 kb on + strand, within EX28DRAFT_3965 at 173.709 kb on + strand, within EX28DRAFT_3965 at 173.751 kb on + strand, within EX28DRAFT_3965 at 173.752 kb on - strand, within EX28DRAFT_3965 at 173.973 kb on - strand, within EX28DRAFT_3965 at 173.975 kb on - strand, within EX28DRAFT_3965 at 174.038 kb on + strand, within EX28DRAFT_3965 at 174.039 kb on - strand, within EX28DRAFT_3965 at 174.061 kb on + strand, within EX28DRAFT_3965 at 174.224 kb on - strand, within EX28DRAFT_3965 at 174.462 kb on - strand at 174.462 kb on - strand at 174.464 kb on + strand at 174.465 kb on - strand at 174.466 kb on + strand at 174.529 kb on + strand at 174.530 kb on - strand at 174.549 kb on + strand at 174.549 kb on + strand at 174.550 kb on - strand at 174.553 kb on - strand at 174.553 kb on - strand at 174.568 kb on - strand at 174.568 kb on - strand at 174.568 kb on - strand at 174.573 kb on + strand at 174.574 kb on - strand at 174.578 kb on + strand at 174.673 kb on + strand at 174.736 kb on + strand, within EX28DRAFT_3966 at 174.903 kb on + strand, within EX28DRAFT_3966 at 174.922 kb on + strand, within EX28DRAFT_3966 at 174.922 kb on + strand, within EX28DRAFT_3966 at 174.922 kb on + strand, within EX28DRAFT_3966 at 174.923 kb on - strand, within EX28DRAFT_3966 at 174.923 kb on - strand, within EX28DRAFT_3966 at 174.923 kb on - strand, within EX28DRAFT_3966 at 174.989 kb on - strand, within EX28DRAFT_3966 at 175.014 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.120 kb on - strand, within EX28DRAFT_3966 at 175.120 kb on - strand, within EX28DRAFT_3966 at 175.370 kb on - strand, within EX28DRAFT_3966 at 175.376 kb on - strand, within EX28DRAFT_3966 at 175.502 kb on - strand, within EX28DRAFT_3966 at 175.633 kb on + strand, within EX28DRAFT_3966 at 175.658 kb on - strand, within EX28DRAFT_3966 at 175.658 kb on - strand, within EX28DRAFT_3966 at 175.820 kb on - strand at 175.820 kb on - strand at 175.838 kb on - strand at 175.838 kb on - strand at 175.841 kb on + strand at 175.842 kb on - strand at 175.873 kb on + strand at 175.891 kb on + strand at 175.891 kb on + strand at 175.891 kb on + strand at 176.003 kb on - strand, within EX28DRAFT_3967 at 176.078 kb on + strand, within EX28DRAFT_3967 at 176.270 kb on - strand at 176.339 kb on + strand, within EX28DRAFT_3968 at 176.341 kb on + strand, within EX28DRAFT_3968 at 176.648 kb on + strand at 176.648 kb on + strand at 176.649 kb on - strand at 176.718 kb on + strand at 176.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 173,704 + EX28DRAFT_3965 0.38 +0.2 173,704 + EX28DRAFT_3965 0.38 -1.2 173,709 + EX28DRAFT_3965 0.38 +0.8 173,751 + EX28DRAFT_3965 0.41 -1.4 173,752 - EX28DRAFT_3965 0.41 +0.8 173,973 - EX28DRAFT_3965 0.58 -0.0 173,975 - EX28DRAFT_3965 0.58 +0.4 174,038 + EX28DRAFT_3965 0.63 +1.1 174,039 - EX28DRAFT_3965 0.63 +0.1 174,061 + EX28DRAFT_3965 0.64 +0.1 174,224 - EX28DRAFT_3965 0.77 -0.4 174,462 - -0.7 174,462 - +2.1 174,464 + -0.2 174,465 - +0.5 174,466 + -0.2 174,529 + +0.2 174,530 - -0.2 174,549 + -1.6 174,549 + -1.4 174,550 - -0.2 174,553 - -0.6 174,553 - -0.4 174,568 - -0.2 174,568 - -1.0 174,568 - -0.7 174,573 + +0.2 174,574 - -0.4 174,578 + -2.0 174,673 + +1.0 174,736 + EX28DRAFT_3966 0.14 +0.6 174,903 + EX28DRAFT_3966 0.27 -0.5 174,922 + EX28DRAFT_3966 0.29 -1.1 174,922 + EX28DRAFT_3966 0.29 -0.6 174,922 + EX28DRAFT_3966 0.29 -0.5 174,923 - EX28DRAFT_3966 0.29 -0.0 174,923 - EX28DRAFT_3966 0.29 -1.5 174,923 - EX28DRAFT_3966 0.29 +0.3 174,989 - EX28DRAFT_3966 0.34 -1.0 175,014 + EX28DRAFT_3966 0.36 +1.4 175,119 + EX28DRAFT_3966 0.44 -0.4 175,119 + EX28DRAFT_3966 0.44 +0.8 175,119 + EX28DRAFT_3966 0.44 -0.7 175,119 + EX28DRAFT_3966 0.44 -0.6 175,119 + EX28DRAFT_3966 0.44 -0.8 175,120 - EX28DRAFT_3966 0.44 -1.2 175,120 - EX28DRAFT_3966 0.44 +0.2 175,370 - EX28DRAFT_3966 0.63 -0.6 175,376 - EX28DRAFT_3966 0.64 -0.1 175,502 - EX28DRAFT_3966 0.74 -0.3 175,633 + EX28DRAFT_3966 0.84 -0.5 175,658 - EX28DRAFT_3966 0.86 -2.0 175,658 - EX28DRAFT_3966 0.86 -0.6 175,820 - -0.4 175,820 - -0.2 175,838 - +1.1 175,838 - -0.2 175,841 + +1.0 175,842 - +0.8 175,873 + -0.7 175,891 + +0.0 175,891 + +0.6 175,891 + +0.2 176,003 - EX28DRAFT_3967 0.23 +0.4 176,078 + EX28DRAFT_3967 0.45 +0.9 176,270 - +0.1 176,339 + EX28DRAFT_3968 0.23 -0.2 176,341 + EX28DRAFT_3968 0.24 +0.5 176,648 + -0.4 176,648 + +0.7 176,649 - +0.3 176,718 + +0.1 176,719 - -0.1
Or see this region's nucleotide sequence