Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3620 and EX28DRAFT_3621 are separated by 110 nucleotides EX28DRAFT_3621 and EX28DRAFT_3622 are separated by 122 nucleotides EX28DRAFT_3622 and EX28DRAFT_3623 overlap by 24 nucleotides EX28DRAFT_3623 and EX28DRAFT_3624 are separated by 26 nucleotides EX28DRAFT_3624 and EX28DRAFT_3625 are separated by 117 nucleotides
EX28DRAFT_3620: EX28DRAFT_3620 - transaldolase, at 280,176 to 281,129
_3620
EX28DRAFT_3621: EX28DRAFT_3621 - molybdenum cofactor synthesis domain, at 281,240 to 281,827
_3621
EX28DRAFT_3622: EX28DRAFT_3622 - Arabinose efflux permease, at 281,950 to 283,272
_3622
EX28DRAFT_3623: EX28DRAFT_3623 - Predicted membrane protein, at 283,249 to 283,815
_3623
EX28DRAFT_3624: EX28DRAFT_3624 - hypothetical protein, at 283,842 to 283,994
_3624
EX28DRAFT_3625: EX28DRAFT_3625 - chaperone protein DnaK, at 284,112 to 286,025
_3625
Position (kb)
281
282
283
284 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 280.959 kb on - strand, within EX28DRAFT_3620 at 280.969 kb on - strand, within EX28DRAFT_3620 at 281.050 kb on + strand at 281.128 kb on - strand at 281.165 kb on + strand at 281.200 kb on + strand at 281.240 kb on - strand at 281.245 kb on + strand at 281.245 kb on + strand at 281.245 kb on + strand at 281.250 kb on + strand at 281.250 kb on + strand at 281.262 kb on + strand at 281.262 kb on + strand at 281.556 kb on + strand, within EX28DRAFT_3621 at 281.556 kb on + strand, within EX28DRAFT_3621 at 281.556 kb on + strand, within EX28DRAFT_3621 at 281.563 kb on - strand, within EX28DRAFT_3621 at 281.724 kb on + strand, within EX28DRAFT_3621 at 281.735 kb on - strand, within EX28DRAFT_3621 at 281.756 kb on + strand, within EX28DRAFT_3621 at 281.756 kb on + strand, within EX28DRAFT_3621 at 281.757 kb on - strand, within EX28DRAFT_3621 at 281.818 kb on - strand at 281.838 kb on + strand at 281.838 kb on + strand at 281.838 kb on + strand at 281.839 kb on - strand at 281.849 kb on - strand at 281.881 kb on - strand at 281.950 kb on - strand at 281.958 kb on + strand at 281.958 kb on + strand at 281.959 kb on - strand at 281.959 kb on - strand at 281.986 kb on + strand at 282.069 kb on - strand at 282.205 kb on - strand, within EX28DRAFT_3622 at 282.274 kb on + strand, within EX28DRAFT_3622 at 282.274 kb on + strand, within EX28DRAFT_3622 at 282.274 kb on + strand, within EX28DRAFT_3622 at 282.274 kb on + strand, within EX28DRAFT_3622 at 282.274 kb on + strand, within EX28DRAFT_3622 at 282.274 kb on + strand, within EX28DRAFT_3622 at 282.275 kb on - strand, within EX28DRAFT_3622 at 282.275 kb on - strand, within EX28DRAFT_3622 at 282.275 kb on - strand, within EX28DRAFT_3622 at 282.477 kb on - strand, within EX28DRAFT_3622 at 282.565 kb on + strand, within EX28DRAFT_3622 at 282.659 kb on + strand, within EX28DRAFT_3622 at 282.923 kb on - strand, within EX28DRAFT_3622 at 282.964 kb on + strand, within EX28DRAFT_3622 at 283.040 kb on + strand, within EX28DRAFT_3622 at 283.066 kb on + strand, within EX28DRAFT_3622 at 283.067 kb on - strand, within EX28DRAFT_3622 at 283.079 kb on - strand, within EX28DRAFT_3622 at 283.153 kb on + strand at 283.154 kb on - strand at 283.154 kb on - strand at 283.157 kb on + strand at 283.157 kb on + strand at 283.184 kb on + strand at 283.185 kb on - strand at 283.319 kb on + strand, within EX28DRAFT_3623 at 283.324 kb on - strand, within EX28DRAFT_3623 at 283.642 kb on - strand, within EX28DRAFT_3623 at 283.643 kb on + strand, within EX28DRAFT_3623 at 283.644 kb on - strand, within EX28DRAFT_3623 at 283.644 kb on - strand, within EX28DRAFT_3623 at 283.803 kb on + strand at 283.803 kb on + strand at 283.803 kb on + strand at 283.804 kb on - strand at 283.804 kb on - strand at 283.815 kb on + strand at 283.815 kb on + strand at 283.816 kb on - strand at 283.838 kb on + strand at 283.839 kb on - strand at 283.841 kb on + strand at 283.841 kb on + strand at 283.869 kb on + strand, within EX28DRAFT_3624 at 283.894 kb on + strand, within EX28DRAFT_3624 at 283.917 kb on + strand, within EX28DRAFT_3624 at 283.992 kb on + strand at 284.060 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 280,959 - EX28DRAFT_3620 0.82 -0.2 280,969 - EX28DRAFT_3620 0.83 -0.7 281,050 + -0.1 281,128 - -0.3 281,165 + -1.7 281,200 + -1.8 281,240 - -1.7 281,245 + +0.0 281,245 + -0.2 281,245 + -0.3 281,250 + +0.3 281,250 + -0.5 281,262 + +0.4 281,262 + +1.2 281,556 + EX28DRAFT_3621 0.54 +2.1 281,556 + EX28DRAFT_3621 0.54 +0.8 281,556 + EX28DRAFT_3621 0.54 -0.5 281,563 - EX28DRAFT_3621 0.55 -0.1 281,724 + EX28DRAFT_3621 0.82 -0.4 281,735 - EX28DRAFT_3621 0.84 -0.4 281,756 + EX28DRAFT_3621 0.88 +0.0 281,756 + EX28DRAFT_3621 0.88 +0.4 281,757 - EX28DRAFT_3621 0.88 -0.6 281,818 - -0.1 281,838 + +1.1 281,838 + -0.3 281,838 + +0.6 281,839 - +1.3 281,849 - +0.0 281,881 - -0.5 281,950 - -0.9 281,958 + -0.8 281,958 + +1.2 281,959 - -0.1 281,959 - +1.4 281,986 + -0.4 282,069 - +0.2 282,205 - EX28DRAFT_3622 0.19 -0.9 282,274 + EX28DRAFT_3622 0.24 +0.1 282,274 + EX28DRAFT_3622 0.24 +0.3 282,274 + EX28DRAFT_3622 0.24 -0.7 282,274 + EX28DRAFT_3622 0.24 -0.2 282,274 + EX28DRAFT_3622 0.24 -0.3 282,274 + EX28DRAFT_3622 0.24 +0.3 282,275 - EX28DRAFT_3622 0.25 -1.4 282,275 - EX28DRAFT_3622 0.25 -1.0 282,275 - EX28DRAFT_3622 0.25 +1.5 282,477 - EX28DRAFT_3622 0.40 +0.1 282,565 + EX28DRAFT_3622 0.46 -0.8 282,659 + EX28DRAFT_3622 0.54 -0.1 282,923 - EX28DRAFT_3622 0.74 -0.4 282,964 + EX28DRAFT_3622 0.77 +0.1 283,040 + EX28DRAFT_3622 0.82 -0.3 283,066 + EX28DRAFT_3622 0.84 -0.8 283,067 - EX28DRAFT_3622 0.84 -0.7 283,079 - EX28DRAFT_3622 0.85 -0.5 283,153 + +0.1 283,154 - +0.5 283,154 - +0.1 283,157 + -0.5 283,157 + +0.0 283,184 + +0.4 283,185 - +0.3 283,319 + EX28DRAFT_3623 0.12 +0.2 283,324 - EX28DRAFT_3623 0.13 -0.1 283,642 - EX28DRAFT_3623 0.69 +1.1 283,643 + EX28DRAFT_3623 0.69 -0.2 283,644 - EX28DRAFT_3623 0.70 +0.5 283,644 - EX28DRAFT_3623 0.70 +0.1 283,803 + -3.2 283,803 + -0.2 283,803 + -0.1 283,804 - -0.2 283,804 - -0.8 283,815 + -0.4 283,815 + -0.5 283,816 - -0.3 283,838 + -2.7 283,839 - -0.1 283,841 + +0.0 283,841 + +1.1 283,869 + EX28DRAFT_3624 0.18 +0.1 283,894 + EX28DRAFT_3624 0.34 +0.7 283,917 + EX28DRAFT_3624 0.49 +0.5 283,992 + +0.4 284,060 + -0.7
Or see this region's nucleotide sequence