Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3581 and EX28DRAFT_3582 are separated by 20 nucleotides EX28DRAFT_3582 and EX28DRAFT_3583 are separated by 91 nucleotides EX28DRAFT_3583 and EX28DRAFT_3584 are separated by 299 nucleotides
EX28DRAFT_3581: EX28DRAFT_3581 - ribosomal-protein-alanine acetyltransferase, at 238,680 to 239,123
_3581
EX28DRAFT_3582: EX28DRAFT_3582 - noncanonical pyrimidine nucleotidase, YjjG family, at 239,144 to 239,821
_3582
EX28DRAFT_3583: EX28DRAFT_3583 - peptide chain release factor 3, at 239,913 to 241,502
_3583
EX28DRAFT_3584: EX28DRAFT_3584 - Predicted periplasmic or secreted lipoprotein, at 241,802 to 242,419
_3584
Position (kb) 239
240
241
242 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 239.060 kb on + strand, within EX28DRAFT_3581 at 239.065 kb on - strand, within EX28DRAFT_3581 at 239.065 kb on - strand, within EX28DRAFT_3581 at 239.131 kb on + strand at 239.132 kb on - strand at 239.188 kb on + strand at 239.189 kb on - strand at 239.189 kb on - strand at 239.367 kb on + strand, within EX28DRAFT_3582 at 239.586 kb on + strand, within EX28DRAFT_3582 at 239.587 kb on - strand, within EX28DRAFT_3582 at 239.587 kb on - strand, within EX28DRAFT_3582 at 239.590 kb on + strand, within EX28DRAFT_3582 at 239.636 kb on - strand, within EX28DRAFT_3582 at 239.844 kb on + strand at 239.895 kb on - strand at 239.928 kb on + strand at 239.929 kb on - strand at 240.206 kb on + strand, within EX28DRAFT_3583 at 240.459 kb on + strand, within EX28DRAFT_3583 at 240.460 kb on - strand, within EX28DRAFT_3583 at 240.460 kb on - strand, within EX28DRAFT_3583 at 240.468 kb on + strand, within EX28DRAFT_3583 at 240.484 kb on - strand, within EX28DRAFT_3583 at 240.881 kb on + strand, within EX28DRAFT_3583 at 241.367 kb on + strand at 241.419 kb on + strand at 241.419 kb on + strand at 241.419 kb on + strand at 241.420 kb on - strand at 241.500 kb on + strand at 241.576 kb on + strand at 241.647 kb on + strand at 241.647 kb on + strand at 241.648 kb on - strand at 241.653 kb on + strand at 241.686 kb on + strand at 241.746 kb on + strand at 241.764 kb on - strand at 241.764 kb on - strand at 241.764 kb on - strand at 241.767 kb on + strand at 241.951 kb on + strand, within EX28DRAFT_3584 at 241.957 kb on + strand, within EX28DRAFT_3584 at 241.957 kb on + strand, within EX28DRAFT_3584 at 241.958 kb on - strand, within EX28DRAFT_3584 at 241.958 kb on - strand, within EX28DRAFT_3584 at 241.958 kb on - strand, within EX28DRAFT_3584 at 242.175 kb on + strand, within EX28DRAFT_3584 at 242.175 kb on + strand, within EX28DRAFT_3584 at 242.176 kb on - strand, within EX28DRAFT_3584 at 242.176 kb on - strand, within EX28DRAFT_3584 at 242.207 kb on + strand, within EX28DRAFT_3584 at 242.227 kb on + strand, within EX28DRAFT_3584 at 242.227 kb on + strand, within EX28DRAFT_3584 at 242.289 kb on + strand, within EX28DRAFT_3584 at 242.289 kb on + strand, within EX28DRAFT_3584 at 242.289 kb on + strand, within EX28DRAFT_3584 at 242.289 kb on + strand, within EX28DRAFT_3584 at 242.418 kb on - strand at 242.462 kb on + strand at 242.463 kb on - strand at 242.463 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 239,060 + EX28DRAFT_3581 0.86 -0.2 239,065 - EX28DRAFT_3581 0.87 +1.0 239,065 - EX28DRAFT_3581 0.87 -0.3 239,131 + -0.0 239,132 - +0.4 239,188 + +0.2 239,189 - +0.3 239,189 - +0.2 239,367 + EX28DRAFT_3582 0.33 -0.0 239,586 + EX28DRAFT_3582 0.65 -0.3 239,587 - EX28DRAFT_3582 0.65 -0.9 239,587 - EX28DRAFT_3582 0.65 +0.5 239,590 + EX28DRAFT_3582 0.66 -0.4 239,636 - EX28DRAFT_3582 0.73 -0.5 239,844 + -1.1 239,895 - +0.1 239,928 + -2.1 239,929 - -0.2 240,206 + EX28DRAFT_3583 0.18 +0.2 240,459 + EX28DRAFT_3583 0.34 -2.2 240,460 - EX28DRAFT_3583 0.34 -1.8 240,460 - EX28DRAFT_3583 0.34 -0.8 240,468 + EX28DRAFT_3583 0.35 -0.7 240,484 - EX28DRAFT_3583 0.36 +0.1 240,881 + EX28DRAFT_3583 0.61 -0.5 241,367 + +0.4 241,419 + +0.5 241,419 + -0.2 241,419 + -0.3 241,420 - +1.0 241,500 + +0.5 241,576 + +0.0 241,647 + +0.1 241,647 + +0.2 241,648 - +0.0 241,653 + -0.1 241,686 + -0.5 241,746 + -0.5 241,764 - -0.2 241,764 - +0.1 241,764 - +0.2 241,767 + +1.1 241,951 + EX28DRAFT_3584 0.24 -0.6 241,957 + EX28DRAFT_3584 0.25 -0.2 241,957 + EX28DRAFT_3584 0.25 -1.0 241,958 - EX28DRAFT_3584 0.25 +0.5 241,958 - EX28DRAFT_3584 0.25 -0.9 241,958 - EX28DRAFT_3584 0.25 +0.1 242,175 + EX28DRAFT_3584 0.60 -3.1 242,175 + EX28DRAFT_3584 0.60 +1.5 242,176 - EX28DRAFT_3584 0.61 +0.9 242,176 - EX28DRAFT_3584 0.61 +0.2 242,207 + EX28DRAFT_3584 0.66 +1.1 242,227 + EX28DRAFT_3584 0.69 +0.7 242,227 + EX28DRAFT_3584 0.69 +0.7 242,289 + EX28DRAFT_3584 0.79 -0.1 242,289 + EX28DRAFT_3584 0.79 +1.6 242,289 + EX28DRAFT_3584 0.79 -1.1 242,289 + EX28DRAFT_3584 0.79 -0.1 242,418 - -0.2 242,462 + -0.3 242,463 - -0.7 242,463 - +1.0
Or see this region's nucleotide sequence