Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3564 and EX28DRAFT_3565 are separated by 41 nucleotides EX28DRAFT_3565 and EX28DRAFT_3566 are separated by 67 nucleotides EX28DRAFT_3566 and EX28DRAFT_3567 overlap by 10 nucleotides
EX28DRAFT_3564: EX28DRAFT_3564 - Transcriptional regulators, at 227,590 to 228,042
_3564
EX28DRAFT_3565: EX28DRAFT_3565 - transcriptional regulator, TetR family, at 228,084 to 228,725
_3565
EX28DRAFT_3566: EX28DRAFT_3566 - Uncharacterized conserved protein, at 228,793 to 229,266
_3566
EX28DRAFT_3567: EX28DRAFT_3567 - Uncharacterized conserved protein, at 229,257 to 230,033
_3567
Position (kb) 228
229
230 Strain fitness (log2 ratio) -2
-1
0
1
2 at 227.904 kb on - strand, within EX28DRAFT_3564 at 227.976 kb on - strand, within EX28DRAFT_3564 at 228.187 kb on + strand, within EX28DRAFT_3565 at 228.203 kb on - strand, within EX28DRAFT_3565 at 228.203 kb on - strand, within EX28DRAFT_3565 at 228.222 kb on + strand, within EX28DRAFT_3565 at 228.222 kb on + strand, within EX28DRAFT_3565 at 228.222 kb on + strand, within EX28DRAFT_3565 at 228.353 kb on + strand, within EX28DRAFT_3565 at 228.397 kb on + strand, within EX28DRAFT_3565 at 228.398 kb on - strand, within EX28DRAFT_3565 at 228.398 kb on - strand, within EX28DRAFT_3565 at 228.549 kb on - strand, within EX28DRAFT_3565 at 228.554 kb on + strand, within EX28DRAFT_3565 at 228.631 kb on + strand, within EX28DRAFT_3565 at 228.783 kb on - strand at 228.783 kb on - strand at 228.791 kb on + strand at 228.792 kb on - strand at 228.792 kb on - strand at 229.074 kb on + strand, within EX28DRAFT_3566 at 229.095 kb on + strand, within EX28DRAFT_3566 at 229.296 kb on + strand at 229.299 kb on + strand at 229.299 kb on + strand at 229.373 kb on + strand, within EX28DRAFT_3567 at 229.374 kb on - strand, within EX28DRAFT_3567 at 229.527 kb on - strand, within EX28DRAFT_3567 at 229.570 kb on + strand, within EX28DRAFT_3567 at 229.570 kb on + strand, within EX28DRAFT_3567 at 229.571 kb on - strand, within EX28DRAFT_3567 at 229.571 kb on - strand, within EX28DRAFT_3567 at 229.571 kb on - strand, within EX28DRAFT_3567 at 229.571 kb on - strand, within EX28DRAFT_3567 at 229.571 kb on - strand, within EX28DRAFT_3567 at 229.571 kb on - strand, within EX28DRAFT_3567 at 229.626 kb on - strand, within EX28DRAFT_3567 at 229.634 kb on + strand, within EX28DRAFT_3567 at 229.660 kb on - strand, within EX28DRAFT_3567 at 229.660 kb on - strand, within EX28DRAFT_3567 at 229.692 kb on + strand, within EX28DRAFT_3567 at 229.693 kb on - strand, within EX28DRAFT_3567 at 229.745 kb on + strand, within EX28DRAFT_3567 at 229.746 kb on - strand, within EX28DRAFT_3567 at 229.746 kb on - strand, within EX28DRAFT_3567 at 229.746 kb on - strand, within EX28DRAFT_3567 at 229.881 kb on - strand, within EX28DRAFT_3567 at 229.988 kb on + strand at 229.989 kb on - strand at 229.992 kb on + strand at 229.993 kb on - strand at 229.993 kb on - strand at 230.003 kb on + strand at 230.035 kb on + strand at 230.035 kb on + strand at 230.035 kb on + strand at 230.035 kb on + strand at 230.035 kb on + strand at 230.036 kb on - strand at 230.036 kb on - strand at 230.081 kb on + strand at 230.152 kb on + strand at 230.153 kb on - strand at 230.198 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 227,904 - EX28DRAFT_3564 0.69 -0.0 227,976 - EX28DRAFT_3564 0.85 -0.2 228,187 + EX28DRAFT_3565 0.16 +1.0 228,203 - EX28DRAFT_3565 0.19 -0.0 228,203 - EX28DRAFT_3565 0.19 -0.9 228,222 + EX28DRAFT_3565 0.21 -0.5 228,222 + EX28DRAFT_3565 0.21 -0.8 228,222 + EX28DRAFT_3565 0.21 -0.4 228,353 + EX28DRAFT_3565 0.42 +0.4 228,397 + EX28DRAFT_3565 0.49 -0.1 228,398 - EX28DRAFT_3565 0.49 +0.8 228,398 - EX28DRAFT_3565 0.49 +1.1 228,549 - EX28DRAFT_3565 0.72 -2.0 228,554 + EX28DRAFT_3565 0.73 -0.1 228,631 + EX28DRAFT_3565 0.85 +0.5 228,783 - +2.5 228,783 - +0.3 228,791 + -0.4 228,792 - -0.5 228,792 - +0.5 229,074 + EX28DRAFT_3566 0.59 +0.2 229,095 + EX28DRAFT_3566 0.64 -1.4 229,296 + -0.7 229,299 + +0.4 229,299 + +0.2 229,373 + EX28DRAFT_3567 0.15 -0.0 229,374 - EX28DRAFT_3567 0.15 -0.0 229,527 - EX28DRAFT_3567 0.35 +0.3 229,570 + EX28DRAFT_3567 0.40 -0.9 229,570 + EX28DRAFT_3567 0.40 -0.5 229,571 - EX28DRAFT_3567 0.40 -0.3 229,571 - EX28DRAFT_3567 0.40 +0.5 229,571 - EX28DRAFT_3567 0.40 +0.1 229,571 - EX28DRAFT_3567 0.40 +0.5 229,571 - EX28DRAFT_3567 0.40 -0.1 229,571 - EX28DRAFT_3567 0.40 +0.5 229,626 - EX28DRAFT_3567 0.47 -0.6 229,634 + EX28DRAFT_3567 0.49 -0.5 229,660 - EX28DRAFT_3567 0.52 -0.3 229,660 - EX28DRAFT_3567 0.52 +0.4 229,692 + EX28DRAFT_3567 0.56 +0.7 229,693 - EX28DRAFT_3567 0.56 +0.1 229,745 + EX28DRAFT_3567 0.63 -0.0 229,746 - EX28DRAFT_3567 0.63 +1.0 229,746 - EX28DRAFT_3567 0.63 +0.3 229,746 - EX28DRAFT_3567 0.63 -1.1 229,881 - EX28DRAFT_3567 0.80 -0.2 229,988 + -0.2 229,989 - -0.5 229,992 + -0.0 229,993 - -1.2 229,993 - -0.5 230,003 + -0.1 230,035 + -0.9 230,035 + +0.4 230,035 + +1.8 230,035 + -0.1 230,035 + +1.1 230,036 - -1.0 230,036 - -1.3 230,081 + -0.0 230,152 + +0.2 230,153 - +0.1 230,198 - -0.6
Or see this region's nucleotide sequence