Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3542 and EX28DRAFT_3543 are separated by 9 nucleotides EX28DRAFT_3543 and EX28DRAFT_3544 are separated by 65 nucleotides EX28DRAFT_3544 and EX28DRAFT_3545 are separated by 9 nucleotides EX28DRAFT_3545 and EX28DRAFT_3546 are separated by 2 nucleotides
EX28DRAFT_3542: EX28DRAFT_3542 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase, at 206,612 to 207,409
_3542
EX28DRAFT_3543: EX28DRAFT_3543 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase, at 207,419 to 208,222
_3543
EX28DRAFT_3544: EX28DRAFT_3544 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 208,288 to 208,668
_3544
EX28DRAFT_3545: EX28DRAFT_3545 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 208,678 to 209,529
_3545
EX28DRAFT_3546: EX28DRAFT_3546 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 209,532 to 210,998
_3546
Position (kb) 208
209 Strain fitness (log2 ratio) -1
0
1 at 207.333 kb on + strand at 207.333 kb on + strand at 207.334 kb on - strand at 207.334 kb on - strand at 207.334 kb on - strand at 207.543 kb on - strand, within EX28DRAFT_3543 at 207.598 kb on + strand, within EX28DRAFT_3543 at 207.689 kb on - strand, within EX28DRAFT_3543 at 207.825 kb on + strand, within EX28DRAFT_3543 at 207.826 kb on - strand, within EX28DRAFT_3543 at 207.826 kb on - strand, within EX28DRAFT_3543 at 207.984 kb on + strand, within EX28DRAFT_3543 at 208.238 kb on + strand at 208.293 kb on + strand at 208.293 kb on + strand at 208.322 kb on + strand at 208.323 kb on - strand at 208.370 kb on + strand, within EX28DRAFT_3544 at 208.371 kb on - strand, within EX28DRAFT_3544 at 208.479 kb on + strand, within EX28DRAFT_3544 at 208.480 kb on - strand, within EX28DRAFT_3544 at 208.645 kb on - strand at 208.657 kb on - strand at 208.705 kb on - strand at 208.764 kb on - strand, within EX28DRAFT_3545 at 208.829 kb on + strand, within EX28DRAFT_3545 at 208.829 kb on + strand, within EX28DRAFT_3545 at 208.932 kb on - strand, within EX28DRAFT_3545 at 209.018 kb on + strand, within EX28DRAFT_3545 at 209.105 kb on - strand, within EX28DRAFT_3545 at 209.132 kb on + strand, within EX28DRAFT_3545 at 209.157 kb on - strand, within EX28DRAFT_3545 at 209.157 kb on - strand, within EX28DRAFT_3545 at 209.255 kb on + strand, within EX28DRAFT_3545 at 209.261 kb on + strand, within EX28DRAFT_3545 at 209.304 kb on - strand, within EX28DRAFT_3545 at 209.523 kb on + strand at 209.524 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 207,333 + -0.1 207,333 + -0.2 207,334 - +0.2 207,334 - -0.4 207,334 - -0.3 207,543 - EX28DRAFT_3543 0.15 -0.7 207,598 + EX28DRAFT_3543 0.22 +0.6 207,689 - EX28DRAFT_3543 0.34 +0.1 207,825 + EX28DRAFT_3543 0.50 +0.4 207,826 - EX28DRAFT_3543 0.51 +0.1 207,826 - EX28DRAFT_3543 0.51 +0.0 207,984 + EX28DRAFT_3543 0.70 +0.4 208,238 + -0.4 208,293 + +0.5 208,293 + -0.0 208,322 + +0.4 208,323 - +0.1 208,370 + EX28DRAFT_3544 0.22 -1.2 208,371 - EX28DRAFT_3544 0.22 -0.9 208,479 + EX28DRAFT_3544 0.50 -0.3 208,480 - EX28DRAFT_3544 0.50 -0.2 208,645 - -0.1 208,657 - +0.1 208,705 - +0.1 208,764 - EX28DRAFT_3545 0.10 -0.2 208,829 + EX28DRAFT_3545 0.18 +0.1 208,829 + EX28DRAFT_3545 0.18 -0.1 208,932 - EX28DRAFT_3545 0.30 +0.8 209,018 + EX28DRAFT_3545 0.40 -0.3 209,105 - EX28DRAFT_3545 0.50 +0.2 209,132 + EX28DRAFT_3545 0.53 -0.6 209,157 - EX28DRAFT_3545 0.56 -0.2 209,157 - EX28DRAFT_3545 0.56 -0.3 209,255 + EX28DRAFT_3545 0.68 -1.3 209,261 + EX28DRAFT_3545 0.68 -0.3 209,304 - EX28DRAFT_3545 0.73 +0.4 209,523 + -1.0 209,524 - +1.2
Or see this region's nucleotide sequence