Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3480 and EX28DRAFT_3481 are separated by 684 nucleotides EX28DRAFT_3481 and EX28DRAFT_3482 overlap by 4 nucleotides EX28DRAFT_3482 and EX28DRAFT_3483 are separated by 21 nucleotides
EX28DRAFT_3480: EX28DRAFT_3480 - hypothetical protein, at 145,288 to 145,623
_3480
EX28DRAFT_3481: EX28DRAFT_3481 - hypothetical protein, at 146,308 to 146,532
_3481
EX28DRAFT_3482: EX28DRAFT_3482 - hypothetical protein, at 146,529 to 146,897
_3482
EX28DRAFT_3483: EX28DRAFT_3483 - Phage major capsid protein E, at 146,919 to 147,950
_3483
Position (kb) 146
147 Strain fitness (log2 ratio) -1
0
1
2 at 145.318 kb on - strand at 145.365 kb on - strand, within EX28DRAFT_3480 at 145.382 kb on + strand, within EX28DRAFT_3480 at 145.528 kb on - strand, within EX28DRAFT_3480 at 145.561 kb on + strand, within EX28DRAFT_3480 at 145.561 kb on + strand, within EX28DRAFT_3480 at 145.561 kb on + strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.562 kb on - strand, within EX28DRAFT_3480 at 145.673 kb on + strand at 145.673 kb on + strand at 145.675 kb on + strand at 145.675 kb on + strand at 145.676 kb on - strand at 145.680 kb on + strand at 145.706 kb on + strand at 145.714 kb on + strand at 145.743 kb on - strand at 145.761 kb on + strand at 145.764 kb on + strand at 145.764 kb on + strand at 145.765 kb on - strand at 145.843 kb on - strand at 145.865 kb on + strand at 145.866 kb on - strand at 145.952 kb on - strand at 145.998 kb on + strand at 146.010 kb on - strand at 146.130 kb on - strand at 146.399 kb on - strand, within EX28DRAFT_3481 at 146.554 kb on - strand at 146.642 kb on + strand, within EX28DRAFT_3482 at 146.666 kb on + strand, within EX28DRAFT_3482 at 146.669 kb on + strand, within EX28DRAFT_3482 at 146.669 kb on + strand, within EX28DRAFT_3482 at 146.670 kb on - strand, within EX28DRAFT_3482 at 146.670 kb on - strand, within EX28DRAFT_3482 at 146.670 kb on - strand, within EX28DRAFT_3482 at 146.728 kb on - strand, within EX28DRAFT_3482 at 146.747 kb on + strand, within EX28DRAFT_3482 at 146.747 kb on + strand, within EX28DRAFT_3482 at 146.748 kb on - strand, within EX28DRAFT_3482 at 146.765 kb on - strand, within EX28DRAFT_3482 at 146.845 kb on + strand, within EX28DRAFT_3482 at 146.915 kb on + strand at 146.929 kb on + strand at 146.930 kb on - strand at 146.959 kb on + strand at 146.981 kb on + strand at 146.982 kb on - strand at 147.078 kb on + strand, within EX28DRAFT_3483 at 147.078 kb on + strand, within EX28DRAFT_3483 at 147.079 kb on - strand, within EX28DRAFT_3483 at 147.079 kb on - strand, within EX28DRAFT_3483 at 147.144 kb on + strand, within EX28DRAFT_3483 at 147.145 kb on - strand, within EX28DRAFT_3483 at 147.162 kb on + strand, within EX28DRAFT_3483 at 147.169 kb on + strand, within EX28DRAFT_3483 at 147.212 kb on + strand, within EX28DRAFT_3483 at 147.229 kb on + strand, within EX28DRAFT_3483 at 147.242 kb on + strand, within EX28DRAFT_3483 at 147.250 kb on + strand, within EX28DRAFT_3483 at 147.250 kb on + strand, within EX28DRAFT_3483 at 147.250 kb on + strand, within EX28DRAFT_3483 at 147.250 kb on + strand, within EX28DRAFT_3483 at 147.251 kb on - strand, within EX28DRAFT_3483 at 147.251 kb on - strand, within EX28DRAFT_3483 at 147.251 kb on - strand, within EX28DRAFT_3483 at 147.251 kb on - strand, within EX28DRAFT_3483 at 147.251 kb on - strand, within EX28DRAFT_3483 at 147.252 kb on + strand, within EX28DRAFT_3483 at 147.252 kb on + strand, within EX28DRAFT_3483 at 147.253 kb on - strand, within EX28DRAFT_3483 at 147.253 kb on - strand, within EX28DRAFT_3483 at 147.253 kb on - strand, within EX28DRAFT_3483 at 147.294 kb on - strand, within EX28DRAFT_3483 at 147.322 kb on + strand, within EX28DRAFT_3483 at 147.391 kb on + strand, within EX28DRAFT_3483 at 147.392 kb on - strand, within EX28DRAFT_3483 at 147.414 kb on - strand, within EX28DRAFT_3483 at 147.414 kb on - strand, within EX28DRAFT_3483 at 147.426 kb on - strand, within EX28DRAFT_3483 at 147.442 kb on + strand, within EX28DRAFT_3483 at 147.442 kb on + strand, within EX28DRAFT_3483 at 147.443 kb on - strand, within EX28DRAFT_3483 at 147.443 kb on - strand, within EX28DRAFT_3483
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 145,318 - +1.1 145,365 - EX28DRAFT_3480 0.23 -0.1 145,382 + EX28DRAFT_3480 0.28 -0.0 145,528 - EX28DRAFT_3480 0.71 +0.3 145,561 + EX28DRAFT_3480 0.81 +0.2 145,561 + EX28DRAFT_3480 0.81 -0.3 145,561 + EX28DRAFT_3480 0.81 -0.6 145,562 - EX28DRAFT_3480 0.82 +0.2 145,562 - EX28DRAFT_3480 0.82 -0.0 145,562 - EX28DRAFT_3480 0.82 -0.2 145,562 - EX28DRAFT_3480 0.82 -0.3 145,562 - EX28DRAFT_3480 0.82 +0.2 145,562 - EX28DRAFT_3480 0.82 +0.6 145,673 + +0.1 145,673 + +0.3 145,675 + +1.2 145,675 + +0.3 145,676 - +0.5 145,680 + -1.0 145,706 + -0.4 145,714 + -0.2 145,743 - +0.1 145,761 + +0.4 145,764 + +0.4 145,764 + +0.9 145,765 - +0.3 145,843 - +0.0 145,865 + -0.3 145,866 - +0.3 145,952 - -0.1 145,998 + -0.4 146,010 - +0.5 146,130 - +1.4 146,399 - EX28DRAFT_3481 0.40 -0.5 146,554 - -0.5 146,642 + EX28DRAFT_3482 0.31 -0.0 146,666 + EX28DRAFT_3482 0.37 -0.3 146,669 + EX28DRAFT_3482 0.38 -0.0 146,669 + EX28DRAFT_3482 0.38 +0.2 146,670 - EX28DRAFT_3482 0.38 +0.1 146,670 - EX28DRAFT_3482 0.38 +0.3 146,670 - EX28DRAFT_3482 0.38 -0.4 146,728 - EX28DRAFT_3482 0.54 -0.2 146,747 + EX28DRAFT_3482 0.59 +0.8 146,747 + EX28DRAFT_3482 0.59 +0.0 146,748 - EX28DRAFT_3482 0.59 +0.4 146,765 - EX28DRAFT_3482 0.64 +0.3 146,845 + EX28DRAFT_3482 0.86 +0.1 146,915 + -0.8 146,929 + -0.8 146,930 - -0.0 146,959 + -0.1 146,981 + +1.8 146,982 - -0.2 147,078 + EX28DRAFT_3483 0.15 -0.6 147,078 + EX28DRAFT_3483 0.15 -0.1 147,079 - EX28DRAFT_3483 0.16 +1.1 147,079 - EX28DRAFT_3483 0.16 +0.3 147,144 + EX28DRAFT_3483 0.22 +0.2 147,145 - EX28DRAFT_3483 0.22 -0.1 147,162 + EX28DRAFT_3483 0.24 -0.4 147,169 + EX28DRAFT_3483 0.24 +0.3 147,212 + EX28DRAFT_3483 0.28 -1.6 147,229 + EX28DRAFT_3483 0.30 +0.0 147,242 + EX28DRAFT_3483 0.31 +0.3 147,250 + EX28DRAFT_3483 0.32 +0.6 147,250 + EX28DRAFT_3483 0.32 +0.6 147,250 + EX28DRAFT_3483 0.32 -0.3 147,250 + EX28DRAFT_3483 0.32 -0.4 147,251 - EX28DRAFT_3483 0.32 -0.2 147,251 - EX28DRAFT_3483 0.32 -0.5 147,251 - EX28DRAFT_3483 0.32 -0.3 147,251 - EX28DRAFT_3483 0.32 -0.4 147,251 - EX28DRAFT_3483 0.32 +0.3 147,252 + EX28DRAFT_3483 0.32 +0.8 147,252 + EX28DRAFT_3483 0.32 -0.9 147,253 - EX28DRAFT_3483 0.32 +0.3 147,253 - EX28DRAFT_3483 0.32 +0.6 147,253 - EX28DRAFT_3483 0.32 +0.4 147,294 - EX28DRAFT_3483 0.36 +1.3 147,322 + EX28DRAFT_3483 0.39 +0.8 147,391 + EX28DRAFT_3483 0.46 +0.6 147,392 - EX28DRAFT_3483 0.46 -0.4 147,414 - EX28DRAFT_3483 0.48 +1.7 147,414 - EX28DRAFT_3483 0.48 -0.1 147,426 - EX28DRAFT_3483 0.49 +0.2 147,442 + EX28DRAFT_3483 0.51 +0.3 147,442 + EX28DRAFT_3483 0.51 -0.4 147,443 - EX28DRAFT_3483 0.51 +0.6 147,443 - EX28DRAFT_3483 0.51 +1.8
Or see this region's nucleotide sequence