Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3433 and EX28DRAFT_3434 are separated by 77 nucleotides EX28DRAFT_3434 and EX28DRAFT_3435 overlap by 11 nucleotides EX28DRAFT_3435 and EX28DRAFT_3436 are separated by 3 nucleotides EX28DRAFT_3436 and EX28DRAFT_3437 are separated by 97 nucleotides
EX28DRAFT_3433: EX28DRAFT_3433 - Transcriptional antiterminator, at 102,489 to 104,399
_3433
EX28DRAFT_3434: EX28DRAFT_3434 - addiction module antitoxin, RelB/DinJ family, at 104,477 to 104,719
_3434
EX28DRAFT_3435: EX28DRAFT_3435 - addiction module toxin, RelE/StbE family, at 104,709 to 104,993
_3435
EX28DRAFT_3436: EX28DRAFT_3436 - anaerobic ribonucleoside-triphosphate reductase activating protein, at 104,997 to 105,461
_3436
EX28DRAFT_3437: EX28DRAFT_3437 - ribonucleoside-triphosphate reductase class III catalytic subunit (EC 1.17.4.2), at 105,559 to 107,697
_3437
Position (kb) 104
105 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 103.523 kb on + strand, within EX28DRAFT_3433 at 103.557 kb on - strand, within EX28DRAFT_3433 at 103.590 kb on + strand, within EX28DRAFT_3433 at 103.599 kb on + strand, within EX28DRAFT_3433 at 103.600 kb on - strand, within EX28DRAFT_3433 at 103.600 kb on - strand, within EX28DRAFT_3433 at 103.640 kb on + strand, within EX28DRAFT_3433 at 103.641 kb on - strand, within EX28DRAFT_3433 at 103.665 kb on + strand, within EX28DRAFT_3433 at 103.906 kb on - strand, within EX28DRAFT_3433 at 103.967 kb on + strand, within EX28DRAFT_3433 at 103.967 kb on + strand, within EX28DRAFT_3433 at 103.971 kb on + strand, within EX28DRAFT_3433 at 103.971 kb on + strand, within EX28DRAFT_3433 at 104.179 kb on + strand, within EX28DRAFT_3433 at 104.184 kb on + strand, within EX28DRAFT_3433 at 104.184 kb on + strand, within EX28DRAFT_3433 at 104.187 kb on - strand, within EX28DRAFT_3433 at 104.339 kb on + strand at 104.465 kb on - strand at 104.476 kb on - strand at 104.586 kb on - strand, within EX28DRAFT_3434 at 104.630 kb on - strand, within EX28DRAFT_3434 at 104.696 kb on - strand at 104.696 kb on - strand at 104.701 kb on + strand at 104.715 kb on + strand at 104.716 kb on - strand at 104.773 kb on + strand, within EX28DRAFT_3435 at 104.774 kb on - strand, within EX28DRAFT_3435 at 104.785 kb on + strand, within EX28DRAFT_3435 at 104.786 kb on - strand, within EX28DRAFT_3435 at 104.786 kb on - strand, within EX28DRAFT_3435 at 104.926 kb on + strand, within EX28DRAFT_3435 at 104.946 kb on - strand, within EX28DRAFT_3435 at 104.991 kb on + strand at 104.992 kb on - strand at 104.995 kb on + strand at 105.016 kb on - strand at 105.058 kb on - strand, within EX28DRAFT_3436 at 105.140 kb on + strand, within EX28DRAFT_3436 at 105.140 kb on + strand, within EX28DRAFT_3436 at 105.142 kb on + strand, within EX28DRAFT_3436 at 105.142 kb on + strand, within EX28DRAFT_3436 at 105.143 kb on - strand, within EX28DRAFT_3436 at 105.162 kb on - strand, within EX28DRAFT_3436 at 105.162 kb on - strand, within EX28DRAFT_3436 at 105.162 kb on - strand, within EX28DRAFT_3436 at 105.442 kb on + strand at 105.442 kb on + strand at 105.445 kb on + strand at 105.446 kb on - strand at 105.446 kb on - strand at 105.446 kb on - strand at 105.462 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 103,523 + EX28DRAFT_3433 0.54 -3.3 103,557 - EX28DRAFT_3433 0.56 +1.4 103,590 + EX28DRAFT_3433 0.58 +0.0 103,599 + EX28DRAFT_3433 0.58 -1.6 103,600 - EX28DRAFT_3433 0.58 -0.1 103,600 - EX28DRAFT_3433 0.58 -0.0 103,640 + EX28DRAFT_3433 0.60 -0.5 103,641 - EX28DRAFT_3433 0.60 +0.6 103,665 + EX28DRAFT_3433 0.62 +0.3 103,906 - EX28DRAFT_3433 0.74 +0.4 103,967 + EX28DRAFT_3433 0.77 -0.2 103,967 + EX28DRAFT_3433 0.77 -0.1 103,971 + EX28DRAFT_3433 0.78 +0.4 103,971 + EX28DRAFT_3433 0.78 +0.1 104,179 + EX28DRAFT_3433 0.88 -0.4 104,184 + EX28DRAFT_3433 0.89 -2.0 104,184 + EX28DRAFT_3433 0.89 +1.4 104,187 - EX28DRAFT_3433 0.89 +0.5 104,339 + -0.1 104,465 - +1.0 104,476 - -0.5 104,586 - EX28DRAFT_3434 0.45 -0.1 104,630 - EX28DRAFT_3434 0.63 -1.1 104,696 - +0.1 104,696 - +0.4 104,701 + -0.2 104,715 + -0.0 104,716 - -0.2 104,773 + EX28DRAFT_3435 0.22 +0.9 104,774 - EX28DRAFT_3435 0.23 -0.2 104,785 + EX28DRAFT_3435 0.27 -0.2 104,786 - EX28DRAFT_3435 0.27 -1.0 104,786 - EX28DRAFT_3435 0.27 +0.0 104,926 + EX28DRAFT_3435 0.76 +0.2 104,946 - EX28DRAFT_3435 0.83 -0.6 104,991 + +0.2 104,992 - -0.0 104,995 + -0.6 105,016 - +0.7 105,058 - EX28DRAFT_3436 0.13 -0.1 105,140 + EX28DRAFT_3436 0.31 -0.2 105,140 + EX28DRAFT_3436 0.31 +0.3 105,142 + EX28DRAFT_3436 0.31 -0.1 105,142 + EX28DRAFT_3436 0.31 -1.1 105,143 - EX28DRAFT_3436 0.31 -0.8 105,162 - EX28DRAFT_3436 0.35 +1.1 105,162 - EX28DRAFT_3436 0.35 +0.8 105,162 - EX28DRAFT_3436 0.35 +0.8 105,442 + -1.3 105,442 + -0.4 105,445 + -1.2 105,446 - +0.0 105,446 - -1.0 105,446 - -0.2 105,462 - +0.6
Or see this region's nucleotide sequence