Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_3400

Experiment: M9 with casaminos and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_3398 and EX28DRAFT_3399 are separated by 84 nucleotidesEX28DRAFT_3399 and EX28DRAFT_3400 are separated by 98 nucleotidesEX28DRAFT_3400 and EX28DRAFT_3401 are separated by 190 nucleotidesEX28DRAFT_3401 and EX28DRAFT_3402 overlap by 7 nucleotides EX28DRAFT_3398: EX28DRAFT_3398 - Predicted nucleoside-diphosphate-sugar epimerases, at 67,405 to 68,253 _3398 EX28DRAFT_3399: EX28DRAFT_3399 - transcriptional regulator, HxlR family, at 68,338 to 68,724 _3399 EX28DRAFT_3400: EX28DRAFT_3400 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase, at 68,823 to 70,766 _3400 EX28DRAFT_3401: EX28DRAFT_3401 - 3'(2'),5'-bisphosphate nucleotidase, bacterial, at 70,957 to 71,697 _3401 EX28DRAFT_3402: EX28DRAFT_3402 - conserved hypothetical protein YtfJ-family, TIGR01626, at 71,691 to 72,248 _3402 Position (kb) 68 69 70 71Strain fitness (log2 ratio) -2 -1 0 1 2at 67.835 kb on + strand, within EX28DRAFT_3398at 67.835 kb on + strand, within EX28DRAFT_3398at 67.835 kb on + strand, within EX28DRAFT_3398at 68.158 kb on + strand, within EX28DRAFT_3398at 68.159 kb on - strand, within EX28DRAFT_3398at 68.293 kb on + strandat 68.293 kb on + strandat 68.293 kb on + strandat 68.294 kb on - strandat 68.294 kb on - strandat 68.294 kb on - strandat 68.607 kb on - strand, within EX28DRAFT_3399at 68.611 kb on + strand, within EX28DRAFT_3399at 68.612 kb on - strand, within EX28DRAFT_3399at 68.612 kb on - strand, within EX28DRAFT_3399at 68.612 kb on - strand, within EX28DRAFT_3399at 68.612 kb on - strand, within EX28DRAFT_3399at 68.612 kb on - strand, within EX28DRAFT_3399at 68.682 kb on + strand, within EX28DRAFT_3399at 68.683 kb on - strand, within EX28DRAFT_3399at 68.741 kb on + strandat 68.742 kb on - strandat 68.824 kb on + strandat 69.119 kb on + strand, within EX28DRAFT_3400at 69.137 kb on - strand, within EX28DRAFT_3400at 69.141 kb on - strand, within EX28DRAFT_3400at 69.248 kb on - strand, within EX28DRAFT_3400at 69.305 kb on + strand, within EX28DRAFT_3400at 69.307 kb on + strand, within EX28DRAFT_3400at 69.307 kb on + strand, within EX28DRAFT_3400at 69.348 kb on - strand, within EX28DRAFT_3400at 69.348 kb on - strand, within EX28DRAFT_3400at 69.426 kb on + strand, within EX28DRAFT_3400at 69.427 kb on - strand, within EX28DRAFT_3400at 69.454 kb on + strand, within EX28DRAFT_3400at 69.505 kb on + strand, within EX28DRAFT_3400at 69.505 kb on + strand, within EX28DRAFT_3400at 69.506 kb on - strand, within EX28DRAFT_3400at 69.506 kb on - strand, within EX28DRAFT_3400at 69.506 kb on - strand, within EX28DRAFT_3400at 69.506 kb on - strand, within EX28DRAFT_3400at 69.611 kb on - strand, within EX28DRAFT_3400at 69.676 kb on + strand, within EX28DRAFT_3400at 69.676 kb on + strand, within EX28DRAFT_3400at 69.677 kb on - strand, within EX28DRAFT_3400at 70.039 kb on + strand, within EX28DRAFT_3400at 70.040 kb on - strand, within EX28DRAFT_3400at 70.144 kb on + strand, within EX28DRAFT_3400at 70.145 kb on - strand, within EX28DRAFT_3400at 70.394 kb on - strand, within EX28DRAFT_3400at 70.394 kb on - strand, within EX28DRAFT_3400at 70.439 kb on + strand, within EX28DRAFT_3400at 70.440 kb on - strand, within EX28DRAFT_3400at 70.465 kb on + strand, within EX28DRAFT_3400at 70.466 kb on - strand, within EX28DRAFT_3400at 70.510 kb on + strand, within EX28DRAFT_3400at 70.510 kb on + strand, within EX28DRAFT_3400at 70.608 kb on + strandat 70.643 kb on - strandat 70.646 kb on - strandat 70.692 kb on - strandat 70.701 kb on + strandat 70.754 kb on + strandat 70.804 kb on + strandat 70.804 kb on + strandat 70.808 kb on + strandat 70.809 kb on - strandat 70.903 kb on - strandat 70.908 kb on - strandat 71.452 kb on + strand, within EX28DRAFT_3401at 71.452 kb on + strand, within EX28DRAFT_3401at 71.644 kb on + strandat 71.646 kb on + strandat 71.646 kb on + strandat 71.692 kb on + strandat 71.693 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM
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67,835 + EX28DRAFT_3398 0.51 -0.5
67,835 + EX28DRAFT_3398 0.51 +1.6
67,835 + EX28DRAFT_3398 0.51 -0.3
68,158 + EX28DRAFT_3398 0.89 +0.6
68,159 - EX28DRAFT_3398 0.89 +0.1
68,293 + +1.9
68,293 + +0.5
68,293 + +0.6
68,294 - -0.3
68,294 - +0.5
68,294 - +0.2
68,607 - EX28DRAFT_3399 0.70 -0.1
68,611 + EX28DRAFT_3399 0.71 -0.4
68,612 - EX28DRAFT_3399 0.71 +0.2
68,612 - EX28DRAFT_3399 0.71 +0.3
68,612 - EX28DRAFT_3399 0.71 +0.1
68,612 - EX28DRAFT_3399 0.71 -0.4
68,612 - EX28DRAFT_3399 0.71 -1.1
68,682 + EX28DRAFT_3399 0.89 -0.0
68,683 - EX28DRAFT_3399 0.89 -1.3
68,741 + +0.2
68,742 - -0.5
68,824 + -0.2
69,119 + EX28DRAFT_3400 0.15 -0.5
69,137 - EX28DRAFT_3400 0.16 -0.4
69,141 - EX28DRAFT_3400 0.16 -1.0
69,248 - EX28DRAFT_3400 0.22 -0.9
69,305 + EX28DRAFT_3400 0.25 -0.9
69,307 + EX28DRAFT_3400 0.25 +0.0
69,307 + EX28DRAFT_3400 0.25 -0.4
69,348 - EX28DRAFT_3400 0.27 -0.2
69,348 - EX28DRAFT_3400 0.27 -0.2
69,426 + EX28DRAFT_3400 0.31 -0.4
69,427 - EX28DRAFT_3400 0.31 +0.1
69,454 + EX28DRAFT_3400 0.32 -0.1
69,505 + EX28DRAFT_3400 0.35 +0.3
69,505 + EX28DRAFT_3400 0.35 -0.8
69,506 - EX28DRAFT_3400 0.35 -1.2
69,506 - EX28DRAFT_3400 0.35 +0.2
69,506 - EX28DRAFT_3400 0.35 -0.1
69,506 - EX28DRAFT_3400 0.35 +1.1
69,611 - EX28DRAFT_3400 0.41 -0.7
69,676 + EX28DRAFT_3400 0.44 -0.3
69,676 + EX28DRAFT_3400 0.44 -0.3
69,677 - EX28DRAFT_3400 0.44 +0.2
70,039 + EX28DRAFT_3400 0.63 -0.2
70,040 - EX28DRAFT_3400 0.63 -0.3
70,144 + EX28DRAFT_3400 0.68 -0.1
70,145 - EX28DRAFT_3400 0.68 +0.3
70,394 - EX28DRAFT_3400 0.81 -1.8
70,394 - EX28DRAFT_3400 0.81 +0.1
70,439 + EX28DRAFT_3400 0.83 +0.1
70,440 - EX28DRAFT_3400 0.83 -0.2
70,465 + EX28DRAFT_3400 0.84 -1.0
70,466 - EX28DRAFT_3400 0.85 -0.5
70,510 + EX28DRAFT_3400 0.87 -0.1
70,510 + EX28DRAFT_3400 0.87 -0.7
70,608 + +0.0
70,643 - -0.3
70,646 - -0.4
70,692 - +1.7
70,701 + -0.3
70,754 + +0.5
70,804 + -0.0
70,804 + +0.0
70,808 + +1.1
70,809 - +0.2
70,903 - +0.9
70,908 - -0.4
71,452 + EX28DRAFT_3401 0.67 +0.3
71,452 + EX28DRAFT_3401 0.67 -0.7
71,644 + -1.4
71,646 + +0.4
71,646 + -0.5
71,692 + -1.8
71,693 - +0.4

Or see this region's nucleotide sequence