Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3102 and EX28DRAFT_3103 are separated by 102 nucleotides EX28DRAFT_3103 and EX28DRAFT_3104 are separated by 125 nucleotides EX28DRAFT_3104 and EX28DRAFT_3105 are separated by 81 nucleotides EX28DRAFT_3105 and EX28DRAFT_3106 are separated by 67 nucleotides
EX28DRAFT_3102: EX28DRAFT_3102 - RNA polymerase, sigma 70 subunit, RpoD, at 411,885 to 413,868
_3102
EX28DRAFT_3103: EX28DRAFT_3103 - G/U mismatch-specific uracil-DNA glycosylase (EC 3.2.2.-), at 413,971 to 414,477
_3103
EX28DRAFT_3104: EX28DRAFT_3104 - tRNA, at 414,603 to 414,678
_3104
EX28DRAFT_3105: EX28DRAFT_3105 - Ogr/Delta-like zinc finger, at 414,760 to 414,960
_3105
EX28DRAFT_3106: EX28DRAFT_3106 - Phage protein D, at 415,028 to 416,182
_3106
Position (kb) 413
414
415 Strain fitness (log2 ratio) -1
0
1 at 413.917 kb on + strand at 414.144 kb on - strand, within EX28DRAFT_3103 at 414.191 kb on - strand, within EX28DRAFT_3103 at 414.329 kb on - strand, within EX28DRAFT_3103 at 414.354 kb on - strand, within EX28DRAFT_3103 at 414.511 kb on - strand at 414.849 kb on + strand, within EX28DRAFT_3105 at 414.894 kb on + strand, within EX28DRAFT_3105 at 414.905 kb on - strand, within EX28DRAFT_3105 at 414.905 kb on - strand, within EX28DRAFT_3105 at 414.918 kb on + strand, within EX28DRAFT_3105 at 415.018 kb on - strand at 415.046 kb on - strand at 415.079 kb on - strand at 415.090 kb on + strand at 415.090 kb on + strand at 415.232 kb on + strand, within EX28DRAFT_3106 at 415.320 kb on - strand, within EX28DRAFT_3106
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 413,917 + +0.3 414,144 - EX28DRAFT_3103 0.34 -0.2 414,191 - EX28DRAFT_3103 0.43 -0.8 414,329 - EX28DRAFT_3103 0.71 -0.8 414,354 - EX28DRAFT_3103 0.76 -0.4 414,511 - +0.1 414,849 + EX28DRAFT_3105 0.44 -0.7 414,894 + EX28DRAFT_3105 0.67 -0.0 414,905 - EX28DRAFT_3105 0.72 +0.3 414,905 - EX28DRAFT_3105 0.72 +1.1 414,918 + EX28DRAFT_3105 0.79 -1.0 415,018 - -0.2 415,046 - +0.4 415,079 - -0.1 415,090 + -0.1 415,090 + -0.0 415,232 + EX28DRAFT_3106 0.18 +0.0 415,320 - EX28DRAFT_3106 0.25 +0.0
Or see this region's nucleotide sequence