Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2994 and EX28DRAFT_2995 are separated by 82 nucleotides EX28DRAFT_2995 and EX28DRAFT_2996 are separated by 56 nucleotides EX28DRAFT_2996 and EX28DRAFT_2997 overlap by 1 nucleotides EX28DRAFT_2997 and EX28DRAFT_2998 are separated by 138 nucleotides
EX28DRAFT_2994: EX28DRAFT_2994 - Uncharacterized protein conserved in bacteria, at 304,190 to 304,528
_2994
EX28DRAFT_2995: EX28DRAFT_2995 - Protein of unknown function (DUF2884), at 304,611 to 305,327
_2995
EX28DRAFT_2996: EX28DRAFT_2996 - Uncharacterized protein conserved in bacteria, at 305,384 to 305,710
_2996
EX28DRAFT_2997: EX28DRAFT_2997 - tRNA (guanine-N(7)-)-methyltransferase, at 305,710 to 306,429
_2997
EX28DRAFT_2998: EX28DRAFT_2998 - A/G-specific DNA-adenine glycosylase (EC 3.2.2.-), at 306,568 to 307,626
_2998
Position (kb) 305
306 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 304.388 kb on + strand, within EX28DRAFT_2994 at 304.437 kb on + strand, within EX28DRAFT_2994 at 304.437 kb on + strand, within EX28DRAFT_2994 at 304.450 kb on + strand, within EX28DRAFT_2994 at 304.479 kb on + strand, within EX28DRAFT_2994 at 304.489 kb on + strand, within EX28DRAFT_2994 at 304.489 kb on + strand, within EX28DRAFT_2994 at 304.526 kb on + strand at 304.556 kb on + strand at 304.556 kb on + strand at 304.613 kb on - strand at 304.617 kb on - strand at 304.617 kb on - strand at 304.894 kb on + strand, within EX28DRAFT_2995 at 304.895 kb on - strand, within EX28DRAFT_2995 at 305.229 kb on + strand, within EX28DRAFT_2995 at 305.331 kb on - strand at 305.331 kb on - strand at 305.360 kb on + strand at 305.360 kb on + strand at 305.383 kb on - strand at 305.383 kb on - strand at 305.385 kb on + strand at 305.386 kb on - strand at 305.386 kb on - strand at 305.452 kb on + strand, within EX28DRAFT_2996 at 305.452 kb on + strand, within EX28DRAFT_2996 at 305.606 kb on + strand, within EX28DRAFT_2996 at 305.606 kb on + strand, within EX28DRAFT_2996 at 305.607 kb on - strand, within EX28DRAFT_2996 at 305.712 kb on - strand at 305.731 kb on + strand at 305.731 kb on + strand at 305.810 kb on + strand, within EX28DRAFT_2997 at 305.811 kb on - strand, within EX28DRAFT_2997 at 305.811 kb on - strand, within EX28DRAFT_2997 at 305.811 kb on - strand, within EX28DRAFT_2997 at 305.868 kb on + strand, within EX28DRAFT_2997 at 305.868 kb on + strand, within EX28DRAFT_2997 at 305.868 kb on + strand, within EX28DRAFT_2997 at 305.868 kb on + strand, within EX28DRAFT_2997 at 305.869 kb on - strand, within EX28DRAFT_2997 at 305.869 kb on - strand, within EX28DRAFT_2997 at 305.879 kb on + strand, within EX28DRAFT_2997 at 305.902 kb on - strand, within EX28DRAFT_2997 at 305.929 kb on - strand, within EX28DRAFT_2997 at 305.973 kb on + strand, within EX28DRAFT_2997 at 305.977 kb on - strand, within EX28DRAFT_2997 at 305.977 kb on - strand, within EX28DRAFT_2997 at 306.048 kb on + strand, within EX28DRAFT_2997 at 306.492 kb on - strand at 306.492 kb on - strand at 306.492 kb on - strand at 306.517 kb on + strand at 306.557 kb on + strand at 306.622 kb on + strand at 306.622 kb on + strand at 306.623 kb on - strand at 306.630 kb on + strand at 306.671 kb on + strand at 306.671 kb on + strand at 306.672 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 304,388 + EX28DRAFT_2994 0.58 -0.2 304,437 + EX28DRAFT_2994 0.73 +0.3 304,437 + EX28DRAFT_2994 0.73 +0.3 304,450 + EX28DRAFT_2994 0.77 +1.5 304,479 + EX28DRAFT_2994 0.85 +0.3 304,489 + EX28DRAFT_2994 0.88 -0.4 304,489 + EX28DRAFT_2994 0.88 +0.5 304,526 + +0.1 304,556 + -0.5 304,556 + -0.1 304,613 - -0.2 304,617 - +0.1 304,617 - +0.0 304,894 + EX28DRAFT_2995 0.39 +0.0 304,895 - EX28DRAFT_2995 0.40 +0.1 305,229 + EX28DRAFT_2995 0.86 +0.2 305,331 - -0.4 305,331 - +0.1 305,360 + -0.1 305,360 + +0.5 305,383 - +0.2 305,383 - -0.3 305,385 + +0.0 305,386 - -0.0 305,386 - +0.4 305,452 + EX28DRAFT_2996 0.21 +0.1 305,452 + EX28DRAFT_2996 0.21 -3.5 305,606 + EX28DRAFT_2996 0.68 -0.4 305,606 + EX28DRAFT_2996 0.68 +2.1 305,607 - EX28DRAFT_2996 0.68 -0.5 305,712 - +1.0 305,731 + -0.1 305,731 + +0.2 305,810 + EX28DRAFT_2997 0.14 -0.4 305,811 - EX28DRAFT_2997 0.14 +0.2 305,811 - EX28DRAFT_2997 0.14 -0.2 305,811 - EX28DRAFT_2997 0.14 -0.1 305,868 + EX28DRAFT_2997 0.22 -0.1 305,868 + EX28DRAFT_2997 0.22 -1.8 305,868 + EX28DRAFT_2997 0.22 -1.1 305,868 + EX28DRAFT_2997 0.22 -0.5 305,869 - EX28DRAFT_2997 0.22 +0.6 305,869 - EX28DRAFT_2997 0.22 -0.0 305,879 + EX28DRAFT_2997 0.23 -1.0 305,902 - EX28DRAFT_2997 0.27 +0.1 305,929 - EX28DRAFT_2997 0.30 -0.0 305,973 + EX28DRAFT_2997 0.37 -0.0 305,977 - EX28DRAFT_2997 0.37 -0.6 305,977 - EX28DRAFT_2997 0.37 +0.8 306,048 + EX28DRAFT_2997 0.47 +0.5 306,492 - -0.1 306,492 - -0.2 306,492 - -0.5 306,517 + +0.4 306,557 + +0.1 306,622 + +0.1 306,622 + +0.2 306,623 - -1.4 306,630 + -0.1 306,671 + +0.2 306,671 + -0.3 306,672 - -2.5
Or see this region's nucleotide sequence