Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2881 and EX28DRAFT_2882 overlap by 8 nucleotides EX28DRAFT_2882 and EX28DRAFT_2883 are separated by 39 nucleotides
EX28DRAFT_2881: EX28DRAFT_2881 - Predicted SAM-dependent methyltransferase, at 188,258 to 189,358
_2881
EX28DRAFT_2882: EX28DRAFT_2882 - Uncharacterized small membrane protein, at 189,351 to 189,746
_2882
EX28DRAFT_2883: EX28DRAFT_2883 - Transcriptional regulator, at 189,786 to 190,703
_2883
Position (kb) 189
190 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 188.358 kb on - strand at 188.358 kb on - strand at 188.394 kb on + strand, within EX28DRAFT_2881 at 188.555 kb on - strand, within EX28DRAFT_2881 at 188.659 kb on + strand, within EX28DRAFT_2881 at 188.659 kb on + strand, within EX28DRAFT_2881 at 188.680 kb on - strand, within EX28DRAFT_2881 at 188.680 kb on - strand, within EX28DRAFT_2881 at 188.719 kb on - strand, within EX28DRAFT_2881 at 188.719 kb on - strand, within EX28DRAFT_2881 at 188.719 kb on - strand, within EX28DRAFT_2881 at 188.843 kb on - strand, within EX28DRAFT_2881 at 188.868 kb on + strand, within EX28DRAFT_2881 at 188.875 kb on - strand, within EX28DRAFT_2881 at 188.881 kb on + strand, within EX28DRAFT_2881 at 188.882 kb on - strand, within EX28DRAFT_2881 at 188.883 kb on + strand, within EX28DRAFT_2881 at 188.884 kb on - strand, within EX28DRAFT_2881 at 188.911 kb on + strand, within EX28DRAFT_2881 at 188.940 kb on + strand, within EX28DRAFT_2881 at 189.020 kb on - strand, within EX28DRAFT_2881 at 189.237 kb on + strand, within EX28DRAFT_2881 at 189.341 kb on + strand at 189.341 kb on + strand at 189.341 kb on + strand at 189.342 kb on - strand at 189.403 kb on - strand, within EX28DRAFT_2882 at 189.405 kb on + strand, within EX28DRAFT_2882 at 189.444 kb on - strand, within EX28DRAFT_2882 at 189.484 kb on + strand, within EX28DRAFT_2882 at 189.509 kb on + strand, within EX28DRAFT_2882 at 189.548 kb on + strand, within EX28DRAFT_2882 at 189.611 kb on - strand, within EX28DRAFT_2882 at 189.663 kb on + strand, within EX28DRAFT_2882 at 189.666 kb on + strand, within EX28DRAFT_2882 at 189.666 kb on + strand, within EX28DRAFT_2882 at 189.666 kb on + strand, within EX28DRAFT_2882 at 189.666 kb on + strand, within EX28DRAFT_2882 at 189.667 kb on - strand, within EX28DRAFT_2882 at 189.667 kb on - strand, within EX28DRAFT_2882 at 189.667 kb on - strand, within EX28DRAFT_2882 at 189.667 kb on - strand, within EX28DRAFT_2882 at 189.701 kb on + strand, within EX28DRAFT_2882 at 189.751 kb on - strand at 189.751 kb on - strand at 189.788 kb on - strand at 189.797 kb on - strand at 189.867 kb on - strand at 189.901 kb on + strand, within EX28DRAFT_2883 at 189.902 kb on - strand, within EX28DRAFT_2883 at 189.924 kb on + strand, within EX28DRAFT_2883 at 189.925 kb on - strand, within EX28DRAFT_2883 at 189.925 kb on - strand, within EX28DRAFT_2883 at 189.933 kb on - strand, within EX28DRAFT_2883 at 190.070 kb on + strand, within EX28DRAFT_2883 at 190.070 kb on + strand, within EX28DRAFT_2883 at 190.083 kb on + strand, within EX28DRAFT_2883 at 190.084 kb on - strand, within EX28DRAFT_2883 at 190.182 kb on + strand, within EX28DRAFT_2883 at 190.222 kb on + strand, within EX28DRAFT_2883 at 190.635 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 188,358 - -0.2 188,358 - +1.1 188,394 + EX28DRAFT_2881 0.12 +1.6 188,555 - EX28DRAFT_2881 0.27 +0.4 188,659 + EX28DRAFT_2881 0.36 -0.1 188,659 + EX28DRAFT_2881 0.36 -0.6 188,680 - EX28DRAFT_2881 0.38 +0.3 188,680 - EX28DRAFT_2881 0.38 +0.5 188,719 - EX28DRAFT_2881 0.42 -0.6 188,719 - EX28DRAFT_2881 0.42 -1.0 188,719 - EX28DRAFT_2881 0.42 +0.1 188,843 - EX28DRAFT_2881 0.53 -0.8 188,868 + EX28DRAFT_2881 0.55 +0.4 188,875 - EX28DRAFT_2881 0.56 +0.4 188,881 + EX28DRAFT_2881 0.57 +1.0 188,882 - EX28DRAFT_2881 0.57 +0.7 188,883 + EX28DRAFT_2881 0.57 +0.2 188,884 - EX28DRAFT_2881 0.57 -0.3 188,911 + EX28DRAFT_2881 0.59 -0.6 188,940 + EX28DRAFT_2881 0.62 -0.4 189,020 - EX28DRAFT_2881 0.69 -0.7 189,237 + EX28DRAFT_2881 0.89 -0.3 189,341 + +0.7 189,341 + +0.0 189,341 + -0.2 189,342 - -0.3 189,403 - EX28DRAFT_2882 0.13 -0.3 189,405 + EX28DRAFT_2882 0.14 +0.2 189,444 - EX28DRAFT_2882 0.23 +0.6 189,484 + EX28DRAFT_2882 0.34 +0.4 189,509 + EX28DRAFT_2882 0.40 -0.6 189,548 + EX28DRAFT_2882 0.50 +0.9 189,611 - EX28DRAFT_2882 0.66 -0.4 189,663 + EX28DRAFT_2882 0.79 -0.2 189,666 + EX28DRAFT_2882 0.80 -1.0 189,666 + EX28DRAFT_2882 0.80 +0.1 189,666 + EX28DRAFT_2882 0.80 -0.0 189,666 + EX28DRAFT_2882 0.80 +0.1 189,667 - EX28DRAFT_2882 0.80 +0.4 189,667 - EX28DRAFT_2882 0.80 -0.6 189,667 - EX28DRAFT_2882 0.80 -0.4 189,667 - EX28DRAFT_2882 0.80 -0.1 189,701 + EX28DRAFT_2882 0.88 +0.9 189,751 - +0.6 189,751 - +0.5 189,788 - -1.6 189,797 - +0.0 189,867 - -1.9 189,901 + EX28DRAFT_2883 0.13 +0.2 189,902 - EX28DRAFT_2883 0.13 -0.7 189,924 + EX28DRAFT_2883 0.15 +0.3 189,925 - EX28DRAFT_2883 0.15 -0.3 189,925 - EX28DRAFT_2883 0.15 -0.7 189,933 - EX28DRAFT_2883 0.16 -0.2 190,070 + EX28DRAFT_2883 0.31 +3.6 190,070 + EX28DRAFT_2883 0.31 -0.6 190,083 + EX28DRAFT_2883 0.32 +0.9 190,084 - EX28DRAFT_2883 0.32 -0.2 190,182 + EX28DRAFT_2883 0.43 -0.3 190,222 + EX28DRAFT_2883 0.47 -1.6 190,635 - -0.9
Or see this region's nucleotide sequence