Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2771 and EX28DRAFT_2772 are separated by 54 nucleotides EX28DRAFT_2772 and EX28DRAFT_2773 are separated by 72 nucleotides EX28DRAFT_2773 and EX28DRAFT_2774 are separated by 8 nucleotides EX28DRAFT_2774 and EX28DRAFT_2775 are separated by 83 nucleotides EX28DRAFT_2775 and EX28DRAFT_2776 overlap by 4 nucleotides
EX28DRAFT_2771: EX28DRAFT_2771 - AraC-type DNA-binding domain-containing proteins, at 80,405 to 81,169
_2771
EX28DRAFT_2772: EX28DRAFT_2772 - S-ribosylhomocysteine lyase (EC 4.4.1.21)/quorum-sensing autoinducer 2 (AI-2) synthesis protein LuxS, at 81,224 to 81,739
_2772
EX28DRAFT_2773: EX28DRAFT_2773 - RNA, at 81,812 to 81,887
_2773
EX28DRAFT_2774: EX28DRAFT_2774 - glutamate-cysteine ligase (EC 6.3.2.2), at 81,896 to 83,440
_2774
EX28DRAFT_2775: EX28DRAFT_2775 - Predicted membrane protein, at 83,524 to 83,952
_2775
EX28DRAFT_2776: EX28DRAFT_2776 - haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED/beta-phosphoglucomutase family hydrolase, at 83,949 to 84,515
_2776
Position (kb) 81
82
83
84 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 81.225 kb on + strand at 81.225 kb on + strand at 81.226 kb on - strand at 81.226 kb on - strand at 81.226 kb on - strand at 81.348 kb on + strand, within EX28DRAFT_2772 at 81.348 kb on + strand, within EX28DRAFT_2772 at 81.349 kb on - strand, within EX28DRAFT_2772 at 81.352 kb on + strand, within EX28DRAFT_2772 at 81.363 kb on + strand, within EX28DRAFT_2772 at 81.477 kb on + strand, within EX28DRAFT_2772 at 81.477 kb on + strand, within EX28DRAFT_2772 at 81.577 kb on + strand, within EX28DRAFT_2772 at 81.578 kb on - strand, within EX28DRAFT_2772 at 81.683 kb on + strand, within EX28DRAFT_2772 at 81.725 kb on - strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.732 kb on - strand at 81.739 kb on + strand at 81.740 kb on - strand at 81.774 kb on + strand at 81.789 kb on + strand at 81.790 kb on - strand at 81.808 kb on + strand at 81.809 kb on - strand at 81.857 kb on - strand, within EX28DRAFT_2773 at 81.857 kb on - strand, within EX28DRAFT_2773 at 81.859 kb on - strand, within EX28DRAFT_2773 at 81.898 kb on - strand at 82.090 kb on + strand, within EX28DRAFT_2774 at 82.298 kb on - strand, within EX28DRAFT_2774 at 82.467 kb on - strand, within EX28DRAFT_2774 at 82.476 kb on + strand, within EX28DRAFT_2774 at 82.677 kb on + strand, within EX28DRAFT_2774 at 82.700 kb on - strand, within EX28DRAFT_2774 at 82.724 kb on - strand, within EX28DRAFT_2774 at 82.777 kb on - strand, within EX28DRAFT_2774 at 82.914 kb on + strand, within EX28DRAFT_2774 at 82.915 kb on - strand, within EX28DRAFT_2774 at 82.989 kb on + strand, within EX28DRAFT_2774 at 83.121 kb on + strand, within EX28DRAFT_2774 at 83.121 kb on + strand, within EX28DRAFT_2774 at 83.147 kb on - strand, within EX28DRAFT_2774 at 83.206 kb on + strand, within EX28DRAFT_2774 at 83.216 kb on - strand, within EX28DRAFT_2774 at 83.279 kb on - strand, within EX28DRAFT_2774 at 83.329 kb on + strand at 83.426 kb on + strand at 83.463 kb on + strand at 83.464 kb on - strand at 83.464 kb on - strand at 83.469 kb on + strand at 83.470 kb on - strand at 83.479 kb on - strand at 83.525 kb on + strand at 83.525 kb on + strand at 83.541 kb on + strand at 83.541 kb on + strand at 83.542 kb on - strand at 83.549 kb on - strand at 83.549 kb on - strand at 83.653 kb on + strand, within EX28DRAFT_2775 at 83.654 kb on - strand, within EX28DRAFT_2775 at 83.665 kb on + strand, within EX28DRAFT_2775 at 83.665 kb on + strand, within EX28DRAFT_2775 at 83.665 kb on + strand, within EX28DRAFT_2775 at 83.666 kb on - strand, within EX28DRAFT_2775 at 83.681 kb on - strand, within EX28DRAFT_2775 at 83.683 kb on + strand, within EX28DRAFT_2775 at 83.776 kb on - strand, within EX28DRAFT_2775 at 83.802 kb on + strand, within EX28DRAFT_2775 at 83.815 kb on + strand, within EX28DRAFT_2775 at 83.818 kb on + strand, within EX28DRAFT_2775 at 83.821 kb on + strand, within EX28DRAFT_2775 at 83.822 kb on - strand, within EX28DRAFT_2775 at 83.822 kb on - strand, within EX28DRAFT_2775 at 83.822 kb on - strand, within EX28DRAFT_2775 at 84.228 kb on + strand, within EX28DRAFT_2776 at 84.229 kb on - strand, within EX28DRAFT_2776 at 84.229 kb on - strand, within EX28DRAFT_2776 at 84.233 kb on + strand, within EX28DRAFT_2776
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 81,225 + +0.3 81,225 + +0.2 81,226 - +0.0 81,226 - +0.3 81,226 - +0.2 81,348 + EX28DRAFT_2772 0.24 -0.2 81,348 + EX28DRAFT_2772 0.24 +0.1 81,349 - EX28DRAFT_2772 0.24 -0.3 81,352 + EX28DRAFT_2772 0.25 +0.1 81,363 + EX28DRAFT_2772 0.27 -0.2 81,477 + EX28DRAFT_2772 0.49 +0.1 81,477 + EX28DRAFT_2772 0.49 +3.6 81,577 + EX28DRAFT_2772 0.68 +0.2 81,578 - EX28DRAFT_2772 0.69 -0.0 81,683 + EX28DRAFT_2772 0.89 -0.8 81,725 - -0.0 81,728 + -1.2 81,728 + +0.2 81,728 + -0.1 81,728 + -0.6 81,728 + +0.1 81,732 - +0.3 81,739 + +0.1 81,740 - -0.6 81,774 + +2.0 81,789 + -0.2 81,790 - +0.6 81,808 + -1.4 81,809 - -0.6 81,857 - EX28DRAFT_2773 0.59 +2.4 81,857 - EX28DRAFT_2773 0.59 -1.9 81,859 - EX28DRAFT_2773 0.62 +0.2 81,898 - -2.3 82,090 + EX28DRAFT_2774 0.13 -1.6 82,298 - EX28DRAFT_2774 0.26 +0.4 82,467 - EX28DRAFT_2774 0.37 -0.9 82,476 + EX28DRAFT_2774 0.38 -0.8 82,677 + EX28DRAFT_2774 0.51 -0.1 82,700 - EX28DRAFT_2774 0.52 -0.2 82,724 - EX28DRAFT_2774 0.54 -0.3 82,777 - EX28DRAFT_2774 0.57 -1.0 82,914 + EX28DRAFT_2774 0.66 -1.7 82,915 - EX28DRAFT_2774 0.66 -0.9 82,989 + EX28DRAFT_2774 0.71 -0.4 83,121 + EX28DRAFT_2774 0.79 -2.6 83,121 + EX28DRAFT_2774 0.79 +0.4 83,147 - EX28DRAFT_2774 0.81 +0.4 83,206 + EX28DRAFT_2774 0.85 +0.4 83,216 - EX28DRAFT_2774 0.85 +1.0 83,279 - EX28DRAFT_2774 0.90 +0.4 83,329 + -0.3 83,426 + -0.1 83,463 + -1.5 83,464 - -2.9 83,464 - +0.1 83,469 + -0.0 83,470 - +1.0 83,479 - -0.2 83,525 + -0.4 83,525 + -0.3 83,541 + +0.6 83,541 + -0.3 83,542 - +0.5 83,549 - +0.6 83,549 - +0.7 83,653 + EX28DRAFT_2775 0.30 +0.0 83,654 - EX28DRAFT_2775 0.30 +0.5 83,665 + EX28DRAFT_2775 0.33 +0.2 83,665 + EX28DRAFT_2775 0.33 -0.1 83,665 + EX28DRAFT_2775 0.33 +0.5 83,666 - EX28DRAFT_2775 0.33 +0.8 83,681 - EX28DRAFT_2775 0.37 -2.5 83,683 + EX28DRAFT_2775 0.37 -0.4 83,776 - EX28DRAFT_2775 0.59 +1.5 83,802 + EX28DRAFT_2775 0.65 -0.1 83,815 + EX28DRAFT_2775 0.68 +0.3 83,818 + EX28DRAFT_2775 0.69 +0.2 83,821 + EX28DRAFT_2775 0.69 -0.5 83,822 - EX28DRAFT_2775 0.69 -0.1 83,822 - EX28DRAFT_2775 0.69 +0.2 83,822 - EX28DRAFT_2775 0.69 +0.8 84,228 + EX28DRAFT_2776 0.49 -0.6 84,229 - EX28DRAFT_2776 0.49 +0.3 84,229 - EX28DRAFT_2776 0.49 +1.0 84,233 + EX28DRAFT_2776 0.50 +0.4
Or see this region's nucleotide sequence