Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2254 and EX28DRAFT_2255 overlap by 4 nucleotides EX28DRAFT_2255 and EX28DRAFT_2256 are separated by 148 nucleotides EX28DRAFT_2256 and EX28DRAFT_2257 overlap by 1 nucleotides EX28DRAFT_2257 and EX28DRAFT_2258 are separated by 14 nucleotides EX28DRAFT_2258 and EX28DRAFT_2259 are separated by 15 nucleotides
EX28DRAFT_2254: EX28DRAFT_2254 - TolQ protein, at 2,274,723 to 2,275,415
_2254
EX28DRAFT_2255: EX28DRAFT_2255 - tol-pal system-associated acyl-CoA thioesterase, at 2,275,412 to 2,275,816
_2255
EX28DRAFT_2256: EX28DRAFT_2256 - cyd operon protein YbgE, at 2,275,965 to 2,276,255
_2256
EX28DRAFT_2257: EX28DRAFT_2257 - cyd operon protein YbgT, at 2,276,255 to 2,276,368
_2257
EX28DRAFT_2258: EX28DRAFT_2258 - cytochrome d oxidase, subunit II (cydB), at 2,276,383 to 2,277,522
_2258
EX28DRAFT_2259: EX28DRAFT_2259 - Cytochrome bd-type quinol oxidase, subunit 1, at 2,277,538 to 2,279,106
_2259
Position (kb)
2276
2277
2278 Strain fitness (log2 ratio)
-2
-1
0
1 at 2275.464 kb on - strand, within EX28DRAFT_2255 at 2275.682 kb on + strand, within EX28DRAFT_2255 at 2275.735 kb on - strand, within EX28DRAFT_2255 at 2275.735 kb on - strand, within EX28DRAFT_2255 at 2275.780 kb on - strand at 2275.780 kb on - strand at 2275.820 kb on - strand at 2275.867 kb on - strand at 2275.867 kb on - strand at 2275.867 kb on - strand at 2275.867 kb on - strand at 2275.868 kb on + strand at 2275.869 kb on - strand at 2275.963 kb on - strand at 2276.096 kb on + strand, within EX28DRAFT_2256 at 2276.097 kb on - strand, within EX28DRAFT_2256 at 2276.165 kb on + strand, within EX28DRAFT_2256 at 2276.166 kb on - strand, within EX28DRAFT_2256 at 2276.166 kb on - strand, within EX28DRAFT_2256 at 2276.166 kb on - strand, within EX28DRAFT_2256 at 2276.182 kb on - strand, within EX28DRAFT_2256 at 2276.233 kb on + strand at 2276.234 kb on - strand at 2276.381 kb on - strand at 2276.381 kb on - strand at 2276.385 kb on - strand at 2276.388 kb on - strand at 2276.391 kb on + strand at 2276.498 kb on + strand, within EX28DRAFT_2258 at 2276.521 kb on - strand, within EX28DRAFT_2258 at 2276.745 kb on + strand, within EX28DRAFT_2258 at 2278.102 kb on - strand, within EX28DRAFT_2259
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,275,464 - EX28DRAFT_2255 0.13 +0.5 2,275,682 + EX28DRAFT_2255 0.67 -0.8 2,275,735 - EX28DRAFT_2255 0.80 +0.0 2,275,735 - EX28DRAFT_2255 0.80 -0.5 2,275,780 - -1.9 2,275,780 - +0.2 2,275,820 - -0.4 2,275,867 - -0.1 2,275,867 - -0.9 2,275,867 - +0.1 2,275,867 - +0.0 2,275,868 + -0.4 2,275,869 - +0.0 2,275,963 - +0.7 2,276,096 + EX28DRAFT_2256 0.45 +0.2 2,276,097 - EX28DRAFT_2256 0.45 -0.8 2,276,165 + EX28DRAFT_2256 0.69 +1.0 2,276,166 - EX28DRAFT_2256 0.69 +0.2 2,276,166 - EX28DRAFT_2256 0.69 -0.5 2,276,166 - EX28DRAFT_2256 0.69 -0.2 2,276,182 - EX28DRAFT_2256 0.75 -0.7 2,276,233 + +0.7 2,276,234 - -0.4 2,276,381 - +0.2 2,276,381 - -1.1 2,276,385 - -0.1 2,276,388 - -1.8 2,276,391 + -1.5 2,276,498 + EX28DRAFT_2258 0.10 -0.4 2,276,521 - EX28DRAFT_2258 0.12 -1.6 2,276,745 + EX28DRAFT_2258 0.32 -1.0 2,278,102 - EX28DRAFT_2259 0.36 -2.7
Or see this region's nucleotide sequence