Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2183 and EX28DRAFT_2184 are separated by 243 nucleotides EX28DRAFT_2184 and EX28DRAFT_2185 are separated by 122 nucleotides EX28DRAFT_2185 and EX28DRAFT_2186 are separated by 168 nucleotides EX28DRAFT_2186 and EX28DRAFT_2187 are separated by 116 nucleotides
EX28DRAFT_2183: EX28DRAFT_2183 - Predicted SAM-dependent methyltransferase, at 2,206,184 to 2,207,095
_2183
EX28DRAFT_2184: EX28DRAFT_2184 - Protein of unknown function (DUF1471), at 2,207,339 to 2,207,605
_2184
EX28DRAFT_2185: EX28DRAFT_2185 - transcriptional regulator, TraR/DksA family, at 2,207,728 to 2,207,994
_2185
EX28DRAFT_2186: EX28DRAFT_2186 - Protein of unknown function (DUF1471), at 2,208,163 to 2,208,540
_2186
EX28DRAFT_2187: EX28DRAFT_2187 - Anthranilate phosphoribosyltransferase, at 2,208,657 to 2,209,619
_2187
Position (kb)
2207
2208 Strain fitness (log2 ratio)
-1
0
1
2 at 2206.741 kb on - strand, within EX28DRAFT_2183 at 2206.840 kb on - strand, within EX28DRAFT_2183 at 2206.999 kb on - strand, within EX28DRAFT_2183 at 2206.999 kb on - strand, within EX28DRAFT_2183 at 2207.195 kb on + strand at 2207.195 kb on + strand at 2207.196 kb on - strand at 2207.262 kb on + strand at 2207.271 kb on + strand at 2207.348 kb on + strand at 2207.349 kb on - strand at 2207.446 kb on - strand, within EX28DRAFT_2184 at 2207.469 kb on + strand, within EX28DRAFT_2184 at 2207.569 kb on + strand, within EX28DRAFT_2184 at 2207.569 kb on + strand, within EX28DRAFT_2184 at 2207.575 kb on + strand, within EX28DRAFT_2184 at 2207.575 kb on + strand, within EX28DRAFT_2184 at 2207.575 kb on + strand, within EX28DRAFT_2184 at 2207.575 kb on + strand, within EX28DRAFT_2184 at 2207.575 kb on + strand, within EX28DRAFT_2184 at 2207.575 kb on + strand, within EX28DRAFT_2184 at 2207.576 kb on - strand, within EX28DRAFT_2184 at 2207.576 kb on - strand, within EX28DRAFT_2184 at 2207.576 kb on - strand, within EX28DRAFT_2184 at 2207.576 kb on - strand, within EX28DRAFT_2184 at 2207.580 kb on - strand at 2207.599 kb on + strand at 2207.599 kb on + strand at 2207.604 kb on - strand at 2207.604 kb on - strand at 2207.959 kb on - strand, within EX28DRAFT_2185 at 2207.987 kb on + strand at 2208.113 kb on + strand at 2208.146 kb on - strand at 2208.221 kb on + strand, within EX28DRAFT_2186 at 2208.275 kb on + strand, within EX28DRAFT_2186 at 2208.296 kb on - strand, within EX28DRAFT_2186 at 2208.296 kb on - strand, within EX28DRAFT_2186 at 2208.363 kb on + strand, within EX28DRAFT_2186 at 2208.399 kb on - strand, within EX28DRAFT_2186 at 2208.487 kb on - strand, within EX28DRAFT_2186 at 2208.533 kb on - strand at 2208.538 kb on + strand at 2208.538 kb on + strand at 2208.801 kb on - strand, within EX28DRAFT_2187 at 2208.801 kb on - strand, within EX28DRAFT_2187 at 2208.801 kb on - strand, within EX28DRAFT_2187 at 2208.804 kb on - strand, within EX28DRAFT_2187 at 2208.969 kb on - strand, within EX28DRAFT_2187
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,206,741 - EX28DRAFT_2183 0.61 +0.2 2,206,840 - EX28DRAFT_2183 0.72 +2.1 2,206,999 - EX28DRAFT_2183 0.89 +0.2 2,206,999 - EX28DRAFT_2183 0.89 -0.5 2,207,195 + +0.6 2,207,195 + -0.3 2,207,196 - -0.5 2,207,262 + +0.4 2,207,271 + -0.6 2,207,348 + -0.2 2,207,349 - +0.3 2,207,446 - EX28DRAFT_2184 0.40 +0.5 2,207,469 + EX28DRAFT_2184 0.49 -0.3 2,207,569 + EX28DRAFT_2184 0.86 -0.1 2,207,569 + EX28DRAFT_2184 0.86 +0.4 2,207,575 + EX28DRAFT_2184 0.88 -0.3 2,207,575 + EX28DRAFT_2184 0.88 +0.7 2,207,575 + EX28DRAFT_2184 0.88 -0.2 2,207,575 + EX28DRAFT_2184 0.88 -0.1 2,207,575 + EX28DRAFT_2184 0.88 -0.2 2,207,575 + EX28DRAFT_2184 0.88 +0.0 2,207,576 - EX28DRAFT_2184 0.89 +0.5 2,207,576 - EX28DRAFT_2184 0.89 +0.0 2,207,576 - EX28DRAFT_2184 0.89 -1.3 2,207,576 - EX28DRAFT_2184 0.89 +0.0 2,207,580 - -0.3 2,207,599 + -0.4 2,207,599 + -0.7 2,207,604 - +0.7 2,207,604 - -0.1 2,207,959 - EX28DRAFT_2185 0.87 -0.8 2,207,987 + -0.5 2,208,113 + -0.5 2,208,146 - -1.2 2,208,221 + EX28DRAFT_2186 0.15 +1.9 2,208,275 + EX28DRAFT_2186 0.30 -0.2 2,208,296 - EX28DRAFT_2186 0.35 -0.1 2,208,296 - EX28DRAFT_2186 0.35 -0.7 2,208,363 + EX28DRAFT_2186 0.53 +0.0 2,208,399 - EX28DRAFT_2186 0.62 +0.3 2,208,487 - EX28DRAFT_2186 0.86 -1.1 2,208,533 - +0.1 2,208,538 + -0.1 2,208,538 + -1.0 2,208,801 - EX28DRAFT_2187 0.15 -0.7 2,208,801 - EX28DRAFT_2187 0.15 -0.4 2,208,801 - EX28DRAFT_2187 0.15 +1.3 2,208,804 - EX28DRAFT_2187 0.15 -0.3 2,208,969 - EX28DRAFT_2187 0.32 -0.7
Or see this region's nucleotide sequence