Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1998 and EX28DRAFT_1999 are separated by 248 nucleotides EX28DRAFT_1999 and EX28DRAFT_2000 overlap by 8 nucleotides EX28DRAFT_2000 and EX28DRAFT_2001 overlap by 4 nucleotides
EX28DRAFT_1998: EX28DRAFT_1998 - dihydroorotate oxidase A (EC 1.3.3.1), at 2,001,635 to 2,002,645
_1998
EX28DRAFT_1999: EX28DRAFT_1999 - FMN reductase, SsuE family, at 2,002,894 to 2,003,469
_1999
EX28DRAFT_2000: EX28DRAFT_2000 - ABC transporter, substrate-binding protein, aliphatic sulfonates family, at 2,003,462 to 2,004,424
_2000
EX28DRAFT_2001: EX28DRAFT_2001 - alkanesulfonate monooxygenase (EC 1.14.14.5), at 2,004,421 to 2,005,566
_2001
Position (kb)
2002
2003
2004 Strain fitness (log2 ratio)
-1
0
1
2 at 2002.893 kb on + strand at 2002.893 kb on + strand at 2002.893 kb on + strand at 2002.893 kb on + strand at 2002.893 kb on + strand at 2002.894 kb on - strand at 2002.894 kb on - strand at 2002.894 kb on - strand at 2002.906 kb on - strand at 2002.958 kb on - strand, within EX28DRAFT_1999 at 2003.013 kb on + strand, within EX28DRAFT_1999 at 2003.119 kb on - strand, within EX28DRAFT_1999 at 2003.122 kb on + strand, within EX28DRAFT_1999 at 2003.122 kb on + strand, within EX28DRAFT_1999 at 2003.122 kb on + strand, within EX28DRAFT_1999 at 2003.123 kb on - strand, within EX28DRAFT_1999 at 2003.123 kb on - strand, within EX28DRAFT_1999 at 2003.123 kb on - strand, within EX28DRAFT_1999 at 2003.234 kb on + strand, within EX28DRAFT_1999 at 2003.340 kb on + strand, within EX28DRAFT_1999 at 2003.340 kb on + strand, within EX28DRAFT_1999 at 2003.340 kb on + strand, within EX28DRAFT_1999 at 2003.341 kb on - strand, within EX28DRAFT_1999 at 2003.341 kb on - strand, within EX28DRAFT_1999 at 2003.467 kb on + strand at 2003.468 kb on - strand at 2003.509 kb on + strand at 2003.509 kb on + strand at 2003.762 kb on + strand, within EX28DRAFT_2000 at 2003.762 kb on + strand, within EX28DRAFT_2000 at 2003.763 kb on - strand, within EX28DRAFT_2000 at 2003.763 kb on - strand, within EX28DRAFT_2000 at 2003.763 kb on - strand, within EX28DRAFT_2000 at 2003.827 kb on + strand, within EX28DRAFT_2000 at 2003.827 kb on + strand, within EX28DRAFT_2000 at 2003.828 kb on - strand, within EX28DRAFT_2000 at 2003.845 kb on + strand, within EX28DRAFT_2000 at 2004.116 kb on + strand, within EX28DRAFT_2000 at 2004.140 kb on - strand, within EX28DRAFT_2000 at 2004.157 kb on + strand, within EX28DRAFT_2000 at 2004.157 kb on + strand, within EX28DRAFT_2000 at 2004.257 kb on + strand, within EX28DRAFT_2000 at 2004.258 kb on - strand, within EX28DRAFT_2000 at 2004.258 kb on - strand, within EX28DRAFT_2000 at 2004.286 kb on + strand, within EX28DRAFT_2000 at 2004.287 kb on - strand, within EX28DRAFT_2000 at 2004.341 kb on + strand at 2004.374 kb on - strand at 2004.416 kb on - strand at 2004.416 kb on - strand at 2004.446 kb on + strand at 2004.469 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,002,893 + +0.1 2,002,893 + +0.3 2,002,893 + -0.2 2,002,893 + -0.5 2,002,893 + -1.0 2,002,894 - -0.5 2,002,894 - +0.9 2,002,894 - -0.8 2,002,906 - +0.5 2,002,958 - EX28DRAFT_1999 0.11 +0.3 2,003,013 + EX28DRAFT_1999 0.21 +1.2 2,003,119 - EX28DRAFT_1999 0.39 -0.2 2,003,122 + EX28DRAFT_1999 0.40 -0.7 2,003,122 + EX28DRAFT_1999 0.40 +0.2 2,003,122 + EX28DRAFT_1999 0.40 +0.2 2,003,123 - EX28DRAFT_1999 0.40 +0.2 2,003,123 - EX28DRAFT_1999 0.40 -0.1 2,003,123 - EX28DRAFT_1999 0.40 +0.6 2,003,234 + EX28DRAFT_1999 0.59 +0.9 2,003,340 + EX28DRAFT_1999 0.77 +0.3 2,003,340 + EX28DRAFT_1999 0.77 -0.5 2,003,340 + EX28DRAFT_1999 0.77 +0.2 2,003,341 - EX28DRAFT_1999 0.78 +0.2 2,003,341 - EX28DRAFT_1999 0.78 +0.4 2,003,467 + -0.3 2,003,468 - -1.1 2,003,509 + +2.5 2,003,509 + -0.3 2,003,762 + EX28DRAFT_2000 0.31 -0.9 2,003,762 + EX28DRAFT_2000 0.31 -0.3 2,003,763 - EX28DRAFT_2000 0.31 -0.3 2,003,763 - EX28DRAFT_2000 0.31 -0.5 2,003,763 - EX28DRAFT_2000 0.31 +0.0 2,003,827 + EX28DRAFT_2000 0.38 +0.2 2,003,827 + EX28DRAFT_2000 0.38 +0.2 2,003,828 - EX28DRAFT_2000 0.38 +0.5 2,003,845 + EX28DRAFT_2000 0.40 -0.4 2,004,116 + EX28DRAFT_2000 0.68 -0.4 2,004,140 - EX28DRAFT_2000 0.70 -0.6 2,004,157 + EX28DRAFT_2000 0.72 +0.2 2,004,157 + EX28DRAFT_2000 0.72 +0.2 2,004,257 + EX28DRAFT_2000 0.83 +0.1 2,004,258 - EX28DRAFT_2000 0.83 +0.3 2,004,258 - EX28DRAFT_2000 0.83 -0.3 2,004,286 + EX28DRAFT_2000 0.86 -0.8 2,004,287 - EX28DRAFT_2000 0.86 +0.8 2,004,341 + +0.5 2,004,374 - +0.9 2,004,416 - +1.2 2,004,416 - -0.3 2,004,446 + -0.0 2,004,469 + -0.1
Or see this region's nucleotide sequence