Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1764 and EX28DRAFT_1765 are separated by 4 nucleotides EX28DRAFT_1765 and EX28DRAFT_1766 are separated by 116 nucleotides EX28DRAFT_1766 and EX28DRAFT_1767 are separated by 167 nucleotides
EX28DRAFT_1764: EX28DRAFT_1764 - DNA topoisomerase III, bacteria and conjugative plasmid, at 1,778,453 to 1,780,360
_1764
EX28DRAFT_1765: EX28DRAFT_1765 - selenophosphate synthase (EC 2.7.9.3), at 1,780,365 to 1,781,408
_1765
EX28DRAFT_1766: EX28DRAFT_1766 - Nitroreductase, at 1,781,525 to 1,782,076
_1766
EX28DRAFT_1767: EX28DRAFT_1767 - signal peptide peptidase SppA, 67K type, at 1,782,244 to 1,784,100
_1767
Position (kb)
1780
1781
1782 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1779.379 kb on - strand, within EX28DRAFT_1764 at 1779.379 kb on - strand, within EX28DRAFT_1764 at 1779.379 kb on - strand, within EX28DRAFT_1764 at 1779.379 kb on - strand, within EX28DRAFT_1764 at 1779.379 kb on - strand, within EX28DRAFT_1764 at 1779.392 kb on - strand, within EX28DRAFT_1764 at 1779.403 kb on - strand, within EX28DRAFT_1764 at 1779.525 kb on + strand, within EX28DRAFT_1764 at 1779.526 kb on - strand, within EX28DRAFT_1764 at 1779.724 kb on + strand, within EX28DRAFT_1764 at 1779.724 kb on + strand, within EX28DRAFT_1764 at 1779.725 kb on - strand, within EX28DRAFT_1764 at 1780.201 kb on - strand at 1780.237 kb on + strand at 1780.237 kb on + strand at 1780.237 kb on + strand at 1780.237 kb on + strand at 1780.238 kb on - strand at 1780.238 kb on - strand at 1780.300 kb on + strand at 1780.568 kb on - strand, within EX28DRAFT_1765 at 1780.616 kb on + strand, within EX28DRAFT_1765 at 1780.617 kb on - strand, within EX28DRAFT_1765 at 1780.618 kb on + strand, within EX28DRAFT_1765 at 1780.619 kb on - strand, within EX28DRAFT_1765 at 1780.619 kb on - strand, within EX28DRAFT_1765 at 1780.619 kb on - strand, within EX28DRAFT_1765 at 1780.619 kb on - strand, within EX28DRAFT_1765 at 1780.619 kb on - strand, within EX28DRAFT_1765 at 1780.850 kb on + strand, within EX28DRAFT_1765 at 1780.985 kb on + strand, within EX28DRAFT_1765 at 1780.986 kb on - strand, within EX28DRAFT_1765 at 1780.986 kb on - strand, within EX28DRAFT_1765 at 1780.986 kb on - strand, within EX28DRAFT_1765 at 1780.986 kb on - strand, within EX28DRAFT_1765 at 1781.198 kb on + strand, within EX28DRAFT_1765 at 1781.199 kb on - strand, within EX28DRAFT_1765 at 1781.238 kb on + strand, within EX28DRAFT_1765 at 1781.238 kb on + strand, within EX28DRAFT_1765 at 1781.238 kb on + strand, within EX28DRAFT_1765 at 1781.239 kb on - strand, within EX28DRAFT_1765 at 1781.239 kb on - strand, within EX28DRAFT_1765 at 1781.239 kb on - strand, within EX28DRAFT_1765 at 1781.377 kb on + strand at 1781.441 kb on + strand at 1781.463 kb on + strand at 1781.464 kb on - strand at 1781.464 kb on - strand at 1781.467 kb on + strand at 1781.468 kb on - strand at 1781.468 kb on - strand at 1781.468 kb on - strand at 1781.468 kb on - strand at 1781.582 kb on - strand, within EX28DRAFT_1766 at 1781.620 kb on + strand, within EX28DRAFT_1766 at 1781.930 kb on - strand, within EX28DRAFT_1766 at 1782.180 kb on - strand at 1782.196 kb on - strand at 1782.241 kb on + strand at 1782.241 kb on + strand at 1782.241 kb on + strand at 1782.242 kb on - strand at 1782.279 kb on + strand at 1782.376 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,779,379 - EX28DRAFT_1764 0.49 +0.0 1,779,379 - EX28DRAFT_1764 0.49 +1.4 1,779,379 - EX28DRAFT_1764 0.49 -0.2 1,779,379 - EX28DRAFT_1764 0.49 -0.0 1,779,379 - EX28DRAFT_1764 0.49 -0.9 1,779,392 - EX28DRAFT_1764 0.49 -0.5 1,779,403 - EX28DRAFT_1764 0.50 -0.0 1,779,525 + EX28DRAFT_1764 0.56 +1.3 1,779,526 - EX28DRAFT_1764 0.56 -1.1 1,779,724 + EX28DRAFT_1764 0.67 +0.7 1,779,724 + EX28DRAFT_1764 0.67 -0.8 1,779,725 - EX28DRAFT_1764 0.67 -0.4 1,780,201 - -0.0 1,780,237 + +0.1 1,780,237 + -0.3 1,780,237 + -1.6 1,780,237 + -0.3 1,780,238 - +0.1 1,780,238 - -0.7 1,780,300 + +0.5 1,780,568 - EX28DRAFT_1765 0.19 +0.2 1,780,616 + EX28DRAFT_1765 0.24 -2.8 1,780,617 - EX28DRAFT_1765 0.24 -1.5 1,780,618 + EX28DRAFT_1765 0.24 -2.6 1,780,619 - EX28DRAFT_1765 0.24 +0.5 1,780,619 - EX28DRAFT_1765 0.24 +0.3 1,780,619 - EX28DRAFT_1765 0.24 +1.7 1,780,619 - EX28DRAFT_1765 0.24 -0.7 1,780,619 - EX28DRAFT_1765 0.24 -0.4 1,780,850 + EX28DRAFT_1765 0.46 -1.6 1,780,985 + EX28DRAFT_1765 0.59 -0.3 1,780,986 - EX28DRAFT_1765 0.59 -1.5 1,780,986 - EX28DRAFT_1765 0.59 -1.0 1,780,986 - EX28DRAFT_1765 0.59 -2.5 1,780,986 - EX28DRAFT_1765 0.59 +0.2 1,781,198 + EX28DRAFT_1765 0.80 -0.5 1,781,199 - EX28DRAFT_1765 0.80 -0.2 1,781,238 + EX28DRAFT_1765 0.84 -0.5 1,781,238 + EX28DRAFT_1765 0.84 -1.0 1,781,238 + EX28DRAFT_1765 0.84 -0.4 1,781,239 - EX28DRAFT_1765 0.84 -0.7 1,781,239 - EX28DRAFT_1765 0.84 +0.4 1,781,239 - EX28DRAFT_1765 0.84 -0.3 1,781,377 + -0.8 1,781,441 + +0.5 1,781,463 + +1.1 1,781,464 - -1.5 1,781,464 - +1.0 1,781,467 + -0.0 1,781,468 - -0.3 1,781,468 - +1.8 1,781,468 - -0.2 1,781,468 - -0.1 1,781,582 - EX28DRAFT_1766 0.10 +0.4 1,781,620 + EX28DRAFT_1766 0.17 -2.4 1,781,930 - EX28DRAFT_1766 0.73 -0.4 1,782,180 - +1.1 1,782,196 - +1.1 1,782,241 + +2.6 1,782,241 + +0.0 1,782,241 + +0.5 1,782,242 - +0.2 1,782,279 + +1.5 1,782,376 - -1.1
Or see this region's nucleotide sequence