Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1515 and EX28DRAFT_1516 are separated by 29 nucleotides EX28DRAFT_1516 and EX28DRAFT_1517 are separated by 124 nucleotides EX28DRAFT_1517 and EX28DRAFT_1518 overlap by 4 nucleotides
EX28DRAFT_1515: EX28DRAFT_1515 - HNH endonuclease, at 1,529,184 to 1,529,531
_1515
EX28DRAFT_1516: EX28DRAFT_1516 - Outer membrane protein V, at 1,529,561 to 1,530,346
_1516
EX28DRAFT_1517: EX28DRAFT_1517 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 1,530,471 to 1,531,166
_1517
EX28DRAFT_1518: EX28DRAFT_1518 - Signal transduction histidine kinase, at 1,531,163 to 1,532,359
_1518
Position (kb)
1530
1531
1532 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1529.503 kb on + strand at 1529.529 kb on + strand at 1529.563 kb on - strand at 1529.651 kb on - strand, within EX28DRAFT_1516 at 1529.653 kb on - strand, within EX28DRAFT_1516 at 1529.724 kb on + strand, within EX28DRAFT_1516 at 1529.724 kb on + strand, within EX28DRAFT_1516 at 1529.725 kb on - strand, within EX28DRAFT_1516 at 1529.858 kb on - strand, within EX28DRAFT_1516 at 1529.893 kb on - strand, within EX28DRAFT_1516 at 1530.094 kb on - strand, within EX28DRAFT_1516 at 1530.320 kb on - strand at 1530.320 kb on - strand at 1530.320 kb on - strand at 1530.321 kb on + strand at 1530.321 kb on + strand at 1530.321 kb on + strand at 1530.321 kb on + strand at 1530.321 kb on + strand at 1530.322 kb on - strand at 1530.322 kb on - strand at 1530.322 kb on - strand at 1530.322 kb on - strand at 1530.322 kb on - strand at 1530.322 kb on - strand at 1530.322 kb on - strand at 1530.322 kb on - strand at 1530.346 kb on + strand at 1530.346 kb on + strand at 1530.346 kb on + strand at 1530.347 kb on - strand at 1530.347 kb on - strand at 1530.411 kb on - strand at 1530.535 kb on - strand at 1530.622 kb on + strand, within EX28DRAFT_1517 at 1530.622 kb on + strand, within EX28DRAFT_1517 at 1530.684 kb on - strand, within EX28DRAFT_1517 at 1530.684 kb on - strand, within EX28DRAFT_1517 at 1530.768 kb on + strand, within EX28DRAFT_1517 at 1530.768 kb on + strand, within EX28DRAFT_1517 at 1530.768 kb on + strand, within EX28DRAFT_1517 at 1530.769 kb on - strand, within EX28DRAFT_1517 at 1530.947 kb on + strand, within EX28DRAFT_1517 at 1531.015 kb on - strand, within EX28DRAFT_1517 at 1531.018 kb on + strand, within EX28DRAFT_1517 at 1531.018 kb on + strand, within EX28DRAFT_1517 at 1531.077 kb on + strand, within EX28DRAFT_1517 at 1531.078 kb on - strand, within EX28DRAFT_1517 at 1531.079 kb on + strand, within EX28DRAFT_1517 at 1531.169 kb on - strand at 1531.630 kb on + strand, within EX28DRAFT_1518 at 1531.631 kb on - strand, within EX28DRAFT_1518 at 1531.631 kb on - strand, within EX28DRAFT_1518 at 1531.750 kb on - strand, within EX28DRAFT_1518 at 1532.090 kb on + strand, within EX28DRAFT_1518
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,529,503 + +1.0 1,529,529 + -0.2 1,529,563 - +0.1 1,529,651 - EX28DRAFT_1516 0.11 +0.2 1,529,653 - EX28DRAFT_1516 0.12 -0.0 1,529,724 + EX28DRAFT_1516 0.21 -0.9 1,529,724 + EX28DRAFT_1516 0.21 -1.3 1,529,725 - EX28DRAFT_1516 0.21 -0.1 1,529,858 - EX28DRAFT_1516 0.38 -0.5 1,529,893 - EX28DRAFT_1516 0.42 -0.3 1,530,094 - EX28DRAFT_1516 0.68 -0.4 1,530,320 - -0.1 1,530,320 - -0.1 1,530,320 - -0.6 1,530,321 + +0.2 1,530,321 + -0.4 1,530,321 + -0.7 1,530,321 + -1.2 1,530,321 + -1.0 1,530,322 - +0.0 1,530,322 - +0.3 1,530,322 - +0.1 1,530,322 - -0.7 1,530,322 - -0.4 1,530,322 - +0.2 1,530,322 - +0.2 1,530,322 - -0.5 1,530,346 + +0.0 1,530,346 + +0.1 1,530,346 + +0.2 1,530,347 - +0.3 1,530,347 - +0.5 1,530,411 - -0.0 1,530,535 - -0.8 1,530,622 + EX28DRAFT_1517 0.22 -0.3 1,530,622 + EX28DRAFT_1517 0.22 +2.6 1,530,684 - EX28DRAFT_1517 0.31 +1.0 1,530,684 - EX28DRAFT_1517 0.31 -0.5 1,530,768 + EX28DRAFT_1517 0.43 -0.5 1,530,768 + EX28DRAFT_1517 0.43 +0.5 1,530,768 + EX28DRAFT_1517 0.43 -0.2 1,530,769 - EX28DRAFT_1517 0.43 +0.0 1,530,947 + EX28DRAFT_1517 0.68 +0.0 1,531,015 - EX28DRAFT_1517 0.78 -2.5 1,531,018 + EX28DRAFT_1517 0.79 -2.0 1,531,018 + EX28DRAFT_1517 0.79 -1.5 1,531,077 + EX28DRAFT_1517 0.87 +1.2 1,531,078 - EX28DRAFT_1517 0.87 -0.7 1,531,079 + EX28DRAFT_1517 0.87 -1.1 1,531,169 - +0.4 1,531,630 + EX28DRAFT_1518 0.39 -0.2 1,531,631 - EX28DRAFT_1518 0.39 -0.5 1,531,631 - EX28DRAFT_1518 0.39 -1.2 1,531,750 - EX28DRAFT_1518 0.49 -0.6 1,532,090 + EX28DRAFT_1518 0.77 +0.2
Or see this region's nucleotide sequence