Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1491 and EX28DRAFT_1492 overlap by 66 nucleotides EX28DRAFT_1492 and EX28DRAFT_1493 are separated by 130 nucleotides EX28DRAFT_1493 and EX28DRAFT_1494 are separated by 94 nucleotides
EX28DRAFT_1491: EX28DRAFT_1491 - Chloride channel protein EriC, at 1,505,054 to 1,506,364
_1491
EX28DRAFT_1492: EX28DRAFT_1492 - dethiobiotin synthase, at 1,506,299 to 1,506,994
_1492
EX28DRAFT_1493: EX28DRAFT_1493 - Transcriptional regulator/sugar kinase, at 1,507,125 to 1,508,345
_1493
EX28DRAFT_1494: EX28DRAFT_1494 - Transcriptional regulator, at 1,508,440 to 1,509,372
_1494
Position (kb)
1507
1508
1509 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1506.174 kb on + strand, within EX28DRAFT_1491 at 1506.202 kb on + strand, within EX28DRAFT_1491 at 1506.202 kb on + strand, within EX28DRAFT_1491 at 1506.202 kb on + strand, within EX28DRAFT_1491 at 1506.203 kb on - strand, within EX28DRAFT_1491 at 1506.354 kb on + strand at 1506.355 kb on - strand at 1506.724 kb on + strand, within EX28DRAFT_1492 at 1506.725 kb on - strand, within EX28DRAFT_1492 at 1506.886 kb on - strand, within EX28DRAFT_1492 at 1506.961 kb on + strand at 1506.961 kb on + strand at 1506.961 kb on + strand at 1506.961 kb on + strand at 1506.962 kb on - strand at 1506.962 kb on - strand at 1506.973 kb on + strand at 1507.028 kb on + strand at 1507.043 kb on + strand at 1507.043 kb on + strand at 1507.043 kb on + strand at 1507.093 kb on - strand at 1507.162 kb on + strand at 1507.162 kb on + strand at 1507.163 kb on - strand at 1507.163 kb on - strand at 1507.163 kb on - strand at 1507.163 kb on - strand at 1507.236 kb on - strand at 1507.361 kb on + strand, within EX28DRAFT_1493 at 1507.403 kb on + strand, within EX28DRAFT_1493 at 1507.403 kb on + strand, within EX28DRAFT_1493 at 1507.404 kb on - strand, within EX28DRAFT_1493 at 1507.404 kb on - strand, within EX28DRAFT_1493 at 1507.573 kb on + strand, within EX28DRAFT_1493 at 1507.573 kb on + strand, within EX28DRAFT_1493 at 1507.773 kb on - strand, within EX28DRAFT_1493 at 1507.774 kb on + strand, within EX28DRAFT_1493 at 1507.775 kb on - strand, within EX28DRAFT_1493 at 1507.877 kb on + strand, within EX28DRAFT_1493 at 1508.307 kb on - strand at 1508.307 kb on - strand at 1508.334 kb on - strand at 1508.359 kb on - strand at 1508.388 kb on + strand at 1508.420 kb on + strand at 1508.420 kb on + strand at 1508.497 kb on + strand at 1508.498 kb on - strand at 1508.674 kb on + strand, within EX28DRAFT_1494 at 1508.675 kb on - strand, within EX28DRAFT_1494 at 1508.675 kb on - strand, within EX28DRAFT_1494 at 1508.675 kb on - strand, within EX28DRAFT_1494 at 1508.675 kb on - strand, within EX28DRAFT_1494 at 1508.675 kb on - strand, within EX28DRAFT_1494 at 1508.757 kb on - strand, within EX28DRAFT_1494 at 1508.757 kb on - strand, within EX28DRAFT_1494 at 1509.110 kb on + strand, within EX28DRAFT_1494 at 1509.110 kb on + strand, within EX28DRAFT_1494 at 1509.111 kb on - strand, within EX28DRAFT_1494 at 1509.111 kb on - strand, within EX28DRAFT_1494 at 1509.345 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,506,174 + EX28DRAFT_1491 0.85 -0.5 1,506,202 + EX28DRAFT_1491 0.88 -0.4 1,506,202 + EX28DRAFT_1491 0.88 -0.0 1,506,202 + EX28DRAFT_1491 0.88 -0.2 1,506,203 - EX28DRAFT_1491 0.88 -0.6 1,506,354 + +2.0 1,506,355 - +1.8 1,506,724 + EX28DRAFT_1492 0.61 -0.5 1,506,725 - EX28DRAFT_1492 0.61 +0.1 1,506,886 - EX28DRAFT_1492 0.84 -0.2 1,506,961 + -0.4 1,506,961 + +0.1 1,506,961 + +0.1 1,506,961 + +0.6 1,506,962 - -1.8 1,506,962 - -0.5 1,506,973 + -1.2 1,507,028 + -1.2 1,507,043 + -0.3 1,507,043 + -0.4 1,507,043 + +0.2 1,507,093 - +0.1 1,507,162 + -0.8 1,507,162 + -0.4 1,507,163 - -0.5 1,507,163 - -1.0 1,507,163 - +0.5 1,507,163 - +0.4 1,507,236 - -1.2 1,507,361 + EX28DRAFT_1493 0.19 -1.0 1,507,403 + EX28DRAFT_1493 0.23 -1.6 1,507,403 + EX28DRAFT_1493 0.23 -1.6 1,507,404 - EX28DRAFT_1493 0.23 -0.0 1,507,404 - EX28DRAFT_1493 0.23 +0.5 1,507,573 + EX28DRAFT_1493 0.37 -0.7 1,507,573 + EX28DRAFT_1493 0.37 -1.4 1,507,773 - EX28DRAFT_1493 0.53 +0.3 1,507,774 + EX28DRAFT_1493 0.53 -2.0 1,507,775 - EX28DRAFT_1493 0.53 -0.1 1,507,877 + EX28DRAFT_1493 0.62 +0.7 1,508,307 - +0.0 1,508,307 - +0.0 1,508,334 - -0.5 1,508,359 - +0.1 1,508,388 + -0.4 1,508,420 + +1.0 1,508,420 + -1.1 1,508,497 + -0.2 1,508,498 - -1.4 1,508,674 + EX28DRAFT_1494 0.25 -1.8 1,508,675 - EX28DRAFT_1494 0.25 +1.1 1,508,675 - EX28DRAFT_1494 0.25 -0.3 1,508,675 - EX28DRAFT_1494 0.25 +0.1 1,508,675 - EX28DRAFT_1494 0.25 +0.9 1,508,675 - EX28DRAFT_1494 0.25 -1.3 1,508,757 - EX28DRAFT_1494 0.34 -1.3 1,508,757 - EX28DRAFT_1494 0.34 -0.4 1,509,110 + EX28DRAFT_1494 0.72 -0.4 1,509,110 + EX28DRAFT_1494 0.72 -0.2 1,509,111 - EX28DRAFT_1494 0.72 -0.2 1,509,111 - EX28DRAFT_1494 0.72 -1.0 1,509,345 - -0.2
Or see this region's nucleotide sequence