Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1437 and EX28DRAFT_1438 are separated by 72 nucleotides EX28DRAFT_1438 and EX28DRAFT_1439 are separated by 67 nucleotides EX28DRAFT_1439 and EX28DRAFT_1440 are separated by 125 nucleotides
EX28DRAFT_1437: EX28DRAFT_1437 - Uncharacterized membrane protein, at 1,449,072 to 1,449,743
_1437
EX28DRAFT_1438: EX28DRAFT_1438 - Transcriptional regulators, at 1,449,816 to 1,450,271
_1438
EX28DRAFT_1439: EX28DRAFT_1439 - Uncharacterized conserved protein, at 1,450,339 to 1,450,629
_1439
EX28DRAFT_1440: EX28DRAFT_1440 - Universal stress protein UspA and related nucleotide-binding proteins, at 1,450,755 to 1,451,189
_1440
Position (kb)
1449
1450
1451 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1448.836 kb on - strand at 1448.891 kb on - strand at 1448.900 kb on + strand at 1448.913 kb on + strand at 1448.993 kb on + strand at 1449.000 kb on + strand at 1449.000 kb on + strand at 1449.043 kb on - strand at 1449.046 kb on + strand at 1449.082 kb on - strand at 1449.083 kb on + strand at 1449.083 kb on + strand at 1449.122 kb on + strand at 1449.153 kb on - strand, within EX28DRAFT_1437 at 1449.412 kb on + strand, within EX28DRAFT_1437 at 1449.434 kb on - strand, within EX28DRAFT_1437 at 1449.499 kb on - strand, within EX28DRAFT_1437 at 1449.556 kb on + strand, within EX28DRAFT_1437 at 1449.556 kb on + strand, within EX28DRAFT_1437 at 1449.598 kb on - strand, within EX28DRAFT_1437 at 1449.605 kb on + strand, within EX28DRAFT_1437 at 1449.742 kb on - strand at 1449.813 kb on + strand at 1449.836 kb on + strand at 1450.174 kb on + strand, within EX28DRAFT_1438 at 1450.174 kb on + strand, within EX28DRAFT_1438 at 1450.175 kb on - strand, within EX28DRAFT_1438 at 1450.175 kb on - strand, within EX28DRAFT_1438 at 1450.293 kb on - strand at 1450.306 kb on + strand at 1450.364 kb on + strand at 1450.365 kb on - strand at 1450.365 kb on - strand at 1450.421 kb on + strand, within EX28DRAFT_1439 at 1450.546 kb on + strand, within EX28DRAFT_1439 at 1450.547 kb on - strand, within EX28DRAFT_1439 at 1450.547 kb on - strand, within EX28DRAFT_1439 at 1450.547 kb on - strand, within EX28DRAFT_1439 at 1450.646 kb on + strand at 1450.646 kb on + strand at 1450.646 kb on + strand at 1450.647 kb on - strand at 1450.653 kb on - strand at 1450.653 kb on - strand at 1450.660 kb on - strand at 1450.758 kb on + strand at 1450.758 kb on + strand at 1450.758 kb on + strand at 1450.759 kb on - strand at 1450.759 kb on - strand at 1450.761 kb on - strand at 1450.761 kb on - strand at 1450.835 kb on + strand, within EX28DRAFT_1440 at 1450.835 kb on + strand, within EX28DRAFT_1440 at 1450.836 kb on - strand, within EX28DRAFT_1440 at 1450.836 kb on - strand, within EX28DRAFT_1440 at 1450.836 kb on - strand, within EX28DRAFT_1440 at 1450.844 kb on + strand, within EX28DRAFT_1440 at 1450.844 kb on + strand, within EX28DRAFT_1440 at 1450.853 kb on + strand, within EX28DRAFT_1440 at 1450.854 kb on - strand, within EX28DRAFT_1440 at 1450.890 kb on + strand, within EX28DRAFT_1440 at 1450.914 kb on + strand, within EX28DRAFT_1440 at 1451.011 kb on + strand, within EX28DRAFT_1440 at 1451.012 kb on - strand, within EX28DRAFT_1440 at 1451.054 kb on + strand, within EX28DRAFT_1440 at 1451.121 kb on + strand, within EX28DRAFT_1440 at 1451.121 kb on + strand, within EX28DRAFT_1440 at 1451.121 kb on + strand, within EX28DRAFT_1440 at 1451.121 kb on + strand, within EX28DRAFT_1440 at 1451.121 kb on + strand, within EX28DRAFT_1440 at 1451.121 kb on + strand, within EX28DRAFT_1440 at 1451.122 kb on - strand, within EX28DRAFT_1440 at 1451.122 kb on - strand, within EX28DRAFT_1440 at 1451.202 kb on - strand at 1451.213 kb on + strand at 1451.266 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,448,836 - +1.5 1,448,891 - -1.1 1,448,900 + +1.6 1,448,913 + +0.8 1,448,993 + -0.0 1,449,000 + +0.2 1,449,000 + -0.1 1,449,043 - -0.2 1,449,046 + +0.3 1,449,082 - -1.0 1,449,083 + +0.1 1,449,083 + +0.0 1,449,122 + -1.4 1,449,153 - EX28DRAFT_1437 0.12 +0.4 1,449,412 + EX28DRAFT_1437 0.51 +0.6 1,449,434 - EX28DRAFT_1437 0.54 -2.2 1,449,499 - EX28DRAFT_1437 0.64 -1.0 1,449,556 + EX28DRAFT_1437 0.72 -1.8 1,449,556 + EX28DRAFT_1437 0.72 +0.3 1,449,598 - EX28DRAFT_1437 0.78 -1.2 1,449,605 + EX28DRAFT_1437 0.79 +2.7 1,449,742 - -0.3 1,449,813 + +0.9 1,449,836 + -0.9 1,450,174 + EX28DRAFT_1438 0.79 +0.5 1,450,174 + EX28DRAFT_1438 0.79 -2.5 1,450,175 - EX28DRAFT_1438 0.79 -0.1 1,450,175 - EX28DRAFT_1438 0.79 -1.9 1,450,293 - +0.2 1,450,306 + -0.4 1,450,364 + +2.1 1,450,365 - +0.8 1,450,365 - +0.3 1,450,421 + EX28DRAFT_1439 0.28 -1.2 1,450,546 + EX28DRAFT_1439 0.71 +1.6 1,450,547 - EX28DRAFT_1439 0.71 +0.2 1,450,547 - EX28DRAFT_1439 0.71 +1.7 1,450,547 - EX28DRAFT_1439 0.71 +0.0 1,450,646 + -0.5 1,450,646 + -0.0 1,450,646 + -0.4 1,450,647 - +0.7 1,450,653 - -0.9 1,450,653 - +0.3 1,450,660 - +0.4 1,450,758 + -1.1 1,450,758 + -0.2 1,450,758 + -0.4 1,450,759 - -0.0 1,450,759 - +0.4 1,450,761 - +0.1 1,450,761 - -0.2 1,450,835 + EX28DRAFT_1440 0.18 +0.3 1,450,835 + EX28DRAFT_1440 0.18 -0.2 1,450,836 - EX28DRAFT_1440 0.19 +0.9 1,450,836 - EX28DRAFT_1440 0.19 +0.0 1,450,836 - EX28DRAFT_1440 0.19 -0.2 1,450,844 + EX28DRAFT_1440 0.20 -0.7 1,450,844 + EX28DRAFT_1440 0.20 -1.8 1,450,853 + EX28DRAFT_1440 0.23 -0.2 1,450,854 - EX28DRAFT_1440 0.23 +0.9 1,450,890 + EX28DRAFT_1440 0.31 +0.6 1,450,914 + EX28DRAFT_1440 0.37 -0.7 1,451,011 + EX28DRAFT_1440 0.59 -0.2 1,451,012 - EX28DRAFT_1440 0.59 +0.5 1,451,054 + EX28DRAFT_1440 0.69 +0.2 1,451,121 + EX28DRAFT_1440 0.84 +0.0 1,451,121 + EX28DRAFT_1440 0.84 +0.6 1,451,121 + EX28DRAFT_1440 0.84 +0.9 1,451,121 + EX28DRAFT_1440 0.84 -0.5 1,451,121 + EX28DRAFT_1440 0.84 +0.1 1,451,121 + EX28DRAFT_1440 0.84 -0.3 1,451,122 - EX28DRAFT_1440 0.84 +0.2 1,451,122 - EX28DRAFT_1440 0.84 -0.2 1,451,202 - +0.3 1,451,213 + -0.3 1,451,266 - +0.8
Or see this region's nucleotide sequence