Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0526 and EX28DRAFT_0527 are separated by 46 nucleotides EX28DRAFT_0527 and EX28DRAFT_0528 are separated by 58 nucleotides EX28DRAFT_0528 and EX28DRAFT_0529 are separated by 146 nucleotides
EX28DRAFT_0526: EX28DRAFT_0526 - hypothetical protein, at 519,418 to 520,428
_0526
EX28DRAFT_0527: EX28DRAFT_0527 - glucose-1-phosphate thymidylyltransferase, short form, at 520,475 to 521,347
_0527
EX28DRAFT_0528: EX28DRAFT_0528 - Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase, at 521,406 to 522,872
_0528
EX28DRAFT_0529: EX28DRAFT_0529 - 6-phosphogluconate dehydrogenase (decarboxylating), at 523,019 to 524,425
_0529
Position (kb) 521
522
523 Strain fitness (log2 ratio) -1
0
1 at 520.460 kb on + strand at 521.319 kb on + strand at 521.593 kb on - strand, within EX28DRAFT_0528 at 522.123 kb on + strand, within EX28DRAFT_0528 at 522.743 kb on + strand at 522.810 kb on + strand at 522.958 kb on + strand at 522.970 kb on - strand at 523.013 kb on + strand at 523.017 kb on + strand at 523.017 kb on + strand at 523.100 kb on - strand at 523.533 kb on - strand, within EX28DRAFT_0529 at 523.560 kb on - strand, within EX28DRAFT_0529
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 520,460 + +0.9 521,319 + +0.8 521,593 - EX28DRAFT_0528 0.13 -0.8 522,123 + EX28DRAFT_0528 0.49 -0.9 522,743 + -0.2 522,810 + +0.5 522,958 + +0.7 522,970 - +0.1 523,013 + -0.0 523,017 + +0.3 523,017 + -0.2 523,100 - +0.2 523,533 - EX28DRAFT_0529 0.37 +0.8 523,560 - EX28DRAFT_0529 0.38 +0.7
Or see this region's nucleotide sequence