Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0297 and EX28DRAFT_0298 are separated by 8 nucleotides EX28DRAFT_0298 and EX28DRAFT_0299 are separated by 48 nucleotides EX28DRAFT_0299 and EX28DRAFT_0300 are separated by 44 nucleotides EX28DRAFT_0300 and EX28DRAFT_0301 are separated by 13 nucleotides
EX28DRAFT_0297: EX28DRAFT_0297 - amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family, at 259,344 to 260,060
_0297
EX28DRAFT_0298: EX28DRAFT_0298 - ABC-type histidine transport system, ATPase component, at 260,069 to 260,842
_0298
EX28DRAFT_0299: EX28DRAFT_0299 - Acetyltransferases, including N-acetylases of ribosomal proteins, at 260,891 to 261,400
_0299
EX28DRAFT_0300: EX28DRAFT_0300 - TIGR01777 family protein, at 261,445 to 262,338
_0300
EX28DRAFT_0301: EX28DRAFT_0301 - FolB domain, at 262,352 to 262,720
_0301
Position (kb) 260
261
262 Strain fitness (log2 ratio) -2
-1
0
1
2 at 259.919 kb on + strand, within EX28DRAFT_0297 at 259.919 kb on + strand, within EX28DRAFT_0297 at 259.920 kb on - strand, within EX28DRAFT_0297 at 259.920 kb on - strand, within EX28DRAFT_0297 at 259.920 kb on - strand, within EX28DRAFT_0297 at 259.932 kb on + strand, within EX28DRAFT_0297 at 259.933 kb on - strand, within EX28DRAFT_0297 at 259.972 kb on - strand, within EX28DRAFT_0297 at 259.975 kb on + strand, within EX28DRAFT_0297 at 259.975 kb on + strand, within EX28DRAFT_0297 at 259.976 kb on - strand, within EX28DRAFT_0297 at 259.976 kb on - strand, within EX28DRAFT_0297 at 259.986 kb on + strand, within EX28DRAFT_0297 at 259.986 kb on + strand, within EX28DRAFT_0297 at 259.986 kb on + strand, within EX28DRAFT_0297 at 259.987 kb on - strand, within EX28DRAFT_0297 at 259.987 kb on - strand, within EX28DRAFT_0297 at 259.987 kb on - strand, within EX28DRAFT_0297 at 260.010 kb on - strand at 260.036 kb on + strand at 260.038 kb on + strand at 260.039 kb on - strand at 260.086 kb on - strand at 260.173 kb on + strand, within EX28DRAFT_0298 at 260.222 kb on - strand, within EX28DRAFT_0298 at 260.273 kb on - strand, within EX28DRAFT_0298 at 260.275 kb on + strand, within EX28DRAFT_0298 at 260.472 kb on - strand, within EX28DRAFT_0298 at 260.840 kb on + strand at 260.841 kb on - strand at 261.136 kb on + strand, within EX28DRAFT_0299 at 261.137 kb on - strand, within EX28DRAFT_0299 at 261.137 kb on - strand, within EX28DRAFT_0299 at 261.137 kb on - strand, within EX28DRAFT_0299 at 261.137 kb on - strand, within EX28DRAFT_0299 at 261.438 kb on - strand at 261.443 kb on - strand at 261.443 kb on - strand at 261.454 kb on + strand at 261.454 kb on + strand at 261.476 kb on - strand at 261.476 kb on - strand at 261.545 kb on - strand, within EX28DRAFT_0300 at 261.652 kb on - strand, within EX28DRAFT_0300 at 261.729 kb on + strand, within EX28DRAFT_0300 at 262.088 kb on - strand, within EX28DRAFT_0300 at 262.147 kb on - strand, within EX28DRAFT_0300 at 262.339 kb on - strand at 262.339 kb on - strand at 262.353 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 259,919 + EX28DRAFT_0297 0.80 +0.3 259,919 + EX28DRAFT_0297 0.80 -0.7 259,920 - EX28DRAFT_0297 0.80 -1.5 259,920 - EX28DRAFT_0297 0.80 -0.1 259,920 - EX28DRAFT_0297 0.80 +0.0 259,932 + EX28DRAFT_0297 0.82 -0.2 259,933 - EX28DRAFT_0297 0.82 -0.4 259,972 - EX28DRAFT_0297 0.88 +0.3 259,975 + EX28DRAFT_0297 0.88 -0.1 259,975 + EX28DRAFT_0297 0.88 +0.0 259,976 - EX28DRAFT_0297 0.88 +1.9 259,976 - EX28DRAFT_0297 0.88 -0.2 259,986 + EX28DRAFT_0297 0.90 +0.5 259,986 + EX28DRAFT_0297 0.90 +0.3 259,986 + EX28DRAFT_0297 0.90 +0.2 259,987 - EX28DRAFT_0297 0.90 -0.1 259,987 - EX28DRAFT_0297 0.90 +0.5 259,987 - EX28DRAFT_0297 0.90 -0.6 260,010 - +0.3 260,036 + -0.4 260,038 + +0.4 260,039 - +0.2 260,086 - +0.2 260,173 + EX28DRAFT_0298 0.13 +0.7 260,222 - EX28DRAFT_0298 0.20 -0.1 260,273 - EX28DRAFT_0298 0.26 -0.1 260,275 + EX28DRAFT_0298 0.27 -0.3 260,472 - EX28DRAFT_0298 0.52 -2.5 260,840 + -0.4 260,841 - +0.8 261,136 + EX28DRAFT_0299 0.48 +0.3 261,137 - EX28DRAFT_0299 0.48 -0.7 261,137 - EX28DRAFT_0299 0.48 +0.9 261,137 - EX28DRAFT_0299 0.48 -0.0 261,137 - EX28DRAFT_0299 0.48 +0.3 261,438 - -0.8 261,443 - +1.5 261,443 - +0.4 261,454 + +1.0 261,454 + -0.5 261,476 - +0.7 261,476 - -0.1 261,545 - EX28DRAFT_0300 0.11 -0.7 261,652 - EX28DRAFT_0300 0.23 +0.0 261,729 + EX28DRAFT_0300 0.32 -0.2 262,088 - EX28DRAFT_0300 0.72 -0.1 262,147 - EX28DRAFT_0300 0.79 +0.1 262,339 - +2.0 262,339 - -0.8 262,353 + -0.2
Or see this region's nucleotide sequence