Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0284 and EX28DRAFT_0285 are separated by 67 nucleotides EX28DRAFT_0285 and EX28DRAFT_0286 overlap by 1 nucleotides EX28DRAFT_0286 and EX28DRAFT_0287 are separated by 28 nucleotides
EX28DRAFT_0284: EX28DRAFT_0284 - Phosphoglycerate dehydrogenase and related dehydrogenases, at 246,763 to 247,899
_0284
EX28DRAFT_0285: EX28DRAFT_0285 - Aspartate-semialdehyde dehydrogenase, at 247,967 to 248,980
_0285
EX28DRAFT_0286: EX28DRAFT_0286 - tRNA pseudouridine(38-40) synthase, at 248,980 to 249,792
_0286
EX28DRAFT_0287: EX28DRAFT_0287 - Uncharacterized membrane-associated protein, at 249,821 to 250,480
_0287
Position (kb) 247
248
249 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 247.054 kb on + strand, within EX28DRAFT_0284 at 247.309 kb on + strand, within EX28DRAFT_0284 at 247.309 kb on + strand, within EX28DRAFT_0284 at 247.311 kb on + strand, within EX28DRAFT_0284 at 247.312 kb on - strand, within EX28DRAFT_0284 at 247.312 kb on - strand, within EX28DRAFT_0284 at 247.312 kb on - strand, within EX28DRAFT_0284 at 247.366 kb on - strand, within EX28DRAFT_0284 at 247.497 kb on + strand, within EX28DRAFT_0284 at 247.498 kb on - strand, within EX28DRAFT_0284 at 247.560 kb on + strand, within EX28DRAFT_0284 at 247.614 kb on - strand, within EX28DRAFT_0284 at 247.628 kb on + strand, within EX28DRAFT_0284 at 247.711 kb on + strand, within EX28DRAFT_0284 at 247.712 kb on - strand, within EX28DRAFT_0284 at 247.789 kb on + strand at 247.789 kb on + strand at 247.789 kb on + strand at 247.789 kb on + strand at 247.860 kb on + strand at 247.897 kb on + strand at 247.908 kb on + strand at 247.908 kb on + strand at 247.958 kb on + strand at 247.995 kb on + strand at 248.065 kb on + strand at 248.069 kb on + strand, within EX28DRAFT_0285 at 248.069 kb on + strand, within EX28DRAFT_0285 at 248.262 kb on + strand, within EX28DRAFT_0285 at 248.611 kb on + strand, within EX28DRAFT_0285 at 248.975 kb on + strand at 249.264 kb on - strand, within EX28DRAFT_0286 at 249.521 kb on + strand, within EX28DRAFT_0286 at 249.545 kb on - strand, within EX28DRAFT_0286 at 249.777 kb on - strand at 249.806 kb on + strand at 249.828 kb on + strand at 249.828 kb on + strand at 249.828 kb on + strand at 249.860 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 247,054 + EX28DRAFT_0284 0.26 -2.6 247,309 + EX28DRAFT_0284 0.48 -1.8 247,309 + EX28DRAFT_0284 0.48 +1.3 247,311 + EX28DRAFT_0284 0.48 -1.9 247,312 - EX28DRAFT_0284 0.48 -2.9 247,312 - EX28DRAFT_0284 0.48 -1.9 247,312 - EX28DRAFT_0284 0.48 -2.5 247,366 - EX28DRAFT_0284 0.53 -2.5 247,497 + EX28DRAFT_0284 0.65 +0.3 247,498 - EX28DRAFT_0284 0.65 -1.8 247,560 + EX28DRAFT_0284 0.70 -3.1 247,614 - EX28DRAFT_0284 0.75 -2.5 247,628 + EX28DRAFT_0284 0.76 -1.8 247,711 + EX28DRAFT_0284 0.83 -3.0 247,712 - EX28DRAFT_0284 0.83 -4.2 247,789 + -2.9 247,789 + -2.7 247,789 + -2.7 247,789 + -2.5 247,860 + -1.3 247,897 + -0.5 247,908 + +0.4 247,908 + +0.6 247,958 + +1.2 247,995 + -1.7 248,065 + -0.4 248,069 + EX28DRAFT_0285 0.10 -1.4 248,069 + EX28DRAFT_0285 0.10 -1.0 248,262 + EX28DRAFT_0285 0.29 -0.4 248,611 + EX28DRAFT_0285 0.64 -0.4 248,975 + +0.6 249,264 - EX28DRAFT_0286 0.35 -1.7 249,521 + EX28DRAFT_0286 0.67 -1.0 249,545 - EX28DRAFT_0286 0.69 -1.2 249,777 - -1.1 249,806 + -0.2 249,828 + +0.2 249,828 + -0.3 249,828 + -0.1 249,860 - -1.7
Or see this region's nucleotide sequence