Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0275 and EX28DRAFT_0276 are separated by 2 nucleotides EX28DRAFT_0276 and EX28DRAFT_0277 overlap by 1 nucleotides EX28DRAFT_0277 and EX28DRAFT_0278 overlap by 1 nucleotides EX28DRAFT_0278 and EX28DRAFT_0279 are separated by 13 nucleotides EX28DRAFT_0279 and EX28DRAFT_0280 are separated by 57 nucleotides
EX28DRAFT_0275: EX28DRAFT_0275 - chorismate synthase (EC 4.2.3.5), at 237,184 to 238,269
_0275
EX28DRAFT_0276: EX28DRAFT_0276 - Murein endopeptidase, at 238,272 to 239,096
_0276
EX28DRAFT_0277: EX28DRAFT_0277 - Predicted permeases, at 239,096 to 239,905
_0277
EX28DRAFT_0278: EX28DRAFT_0278 - Uncharacterized protein conserved in bacteria, at 239,905 to 240,450
_0278
EX28DRAFT_0279: EX28DRAFT_0279 - YfcL protein, at 240,464 to 240,742
_0279
EX28DRAFT_0280: EX28DRAFT_0280 - tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain, at 240,800 to 242,797
_0280
Position (kb) 239
240 Strain fitness (log2 ratio) -2
-1
0
1 at 238.403 kb on + strand, within EX28DRAFT_0276 at 238.440 kb on - strand, within EX28DRAFT_0276 at 238.444 kb on - strand, within EX28DRAFT_0276 at 238.474 kb on - strand, within EX28DRAFT_0276 at 238.529 kb on + strand, within EX28DRAFT_0276 at 238.529 kb on + strand, within EX28DRAFT_0276 at 238.781 kb on + strand, within EX28DRAFT_0276 at 239.089 kb on + strand at 239.095 kb on - strand at 239.120 kb on - strand at 239.176 kb on + strand at 239.306 kb on + strand, within EX28DRAFT_0277 at 239.320 kb on + strand, within EX28DRAFT_0277 at 239.462 kb on + strand, within EX28DRAFT_0277 at 239.806 kb on + strand, within EX28DRAFT_0277 at 239.903 kb on + strand at 239.913 kb on + strand at 239.913 kb on + strand at 239.913 kb on + strand at 239.924 kb on - strand at 240.007 kb on + strand, within EX28DRAFT_0278 at 240.007 kb on + strand, within EX28DRAFT_0278 at 240.034 kb on + strand, within EX28DRAFT_0278 at 240.034 kb on + strand, within EX28DRAFT_0278 at 240.034 kb on + strand, within EX28DRAFT_0278 at 240.036 kb on + strand, within EX28DRAFT_0278 at 240.065 kb on - strand, within EX28DRAFT_0278 at 240.394 kb on + strand, within EX28DRAFT_0278 at 240.395 kb on - strand, within EX28DRAFT_0278 at 240.395 kb on - strand, within EX28DRAFT_0278 at 240.398 kb on - strand at 240.448 kb on + strand at 240.740 kb on + strand at 240.741 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 238,403 + EX28DRAFT_0276 0.16 +0.4 238,440 - EX28DRAFT_0276 0.20 -0.6 238,444 - EX28DRAFT_0276 0.21 +0.2 238,474 - EX28DRAFT_0276 0.24 -0.2 238,529 + EX28DRAFT_0276 0.31 -0.2 238,529 + EX28DRAFT_0276 0.31 +0.0 238,781 + EX28DRAFT_0276 0.62 +0.2 239,089 + +0.1 239,095 - +0.3 239,120 - +0.8 239,176 + -0.0 239,306 + EX28DRAFT_0277 0.26 +0.4 239,320 + EX28DRAFT_0277 0.28 -1.5 239,462 + EX28DRAFT_0277 0.45 -2.7 239,806 + EX28DRAFT_0277 0.88 -1.3 239,903 + +0.2 239,913 + -0.4 239,913 + -1.7 239,913 + +0.0 239,924 - -0.1 240,007 + EX28DRAFT_0278 0.19 +0.3 240,007 + EX28DRAFT_0278 0.19 -0.0 240,034 + EX28DRAFT_0278 0.24 -0.2 240,034 + EX28DRAFT_0278 0.24 -0.1 240,034 + EX28DRAFT_0278 0.24 -1.5 240,036 + EX28DRAFT_0278 0.24 +0.7 240,065 - EX28DRAFT_0278 0.29 -0.6 240,394 + EX28DRAFT_0278 0.90 -0.6 240,395 - EX28DRAFT_0278 0.90 +0.0 240,395 - EX28DRAFT_0278 0.90 +0.1 240,398 - +0.1 240,448 + +0.0 240,740 + +0.6 240,741 - -0.8
Or see this region's nucleotide sequence