Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00718 and CCNA_03930 are separated by 6 nucleotides CCNA_03930 and CCNA_03931 are separated by 542 nucleotides CCNA_03931 and CCNA_03932 are separated by 2 nucleotides CCNA_03932 and CCNA_03933 are separated by 30 nucleotides CCNA_03933 and CCNA_00719 are separated by 115 nucleotides
CCNA_00718: CCNA_00718 - abortive infection protein, at 771,887 to 772,783
_00718
CCNA_03930: CCNA_03930 - hypothetical protein, at 772,790 to 772,894
_03930
CCNA_03931: CCNA_03931 - hypothetical protein, at 773,437 to 773,730
_03931
CCNA_03932: CCNA_03932 - hypothetical protein, at 773,733 to 773,987
_03932
CCNA_03933: CCNA_03933 - hypothetical protein, at 774,018 to 774,278
_03933
CCNA_00719: CCNA_00719 - type I secretion adaptor protein hlyD, at 774,394 to 775,635
_00719
Position (kb) 773
774 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 772.455 kb on - strand, within CCNA_00718 at 772.455 kb on - strand, within CCNA_00718 at 772.468 kb on + strand, within CCNA_00718 at 772.468 kb on + strand, within CCNA_00718 at 772.468 kb on + strand, within CCNA_00718 at 772.476 kb on - strand, within CCNA_00718 at 772.476 kb on - strand, within CCNA_00718 at 772.476 kb on - strand, within CCNA_00718 at 772.476 kb on - strand, within CCNA_00718 at 772.476 kb on - strand, within CCNA_00718 at 772.488 kb on - strand, within CCNA_00718 at 772.523 kb on - strand, within CCNA_00718 at 772.582 kb on - strand, within CCNA_00718 at 772.596 kb on + strand, within CCNA_00718 at 772.698 kb on - strand at 772.698 kb on - strand at 772.724 kb on + strand at 772.745 kb on - strand at 772.756 kb on - strand at 772.787 kb on + strand at 772.787 kb on + strand at 772.802 kb on + strand, within CCNA_03930 at 772.803 kb on - strand, within CCNA_03930 at 772.805 kb on - strand, within CCNA_03930 at 772.820 kb on + strand, within CCNA_03930 at 772.828 kb on - strand, within CCNA_03930 at 772.847 kb on + strand, within CCNA_03930 at 772.852 kb on + strand, within CCNA_03930 at 772.860 kb on - strand, within CCNA_03930 at 772.866 kb on + strand, within CCNA_03930 at 772.869 kb on - strand, within CCNA_03930 at 772.897 kb on - strand at 772.945 kb on + strand at 772.968 kb on + strand at 772.975 kb on + strand at 772.985 kb on + strand at 772.986 kb on + strand at 772.991 kb on + strand at 773.027 kb on + strand at 773.030 kb on - strand at 773.060 kb on - strand at 773.080 kb on + strand at 773.094 kb on + strand at 773.101 kb on - strand at 773.102 kb on - strand at 773.120 kb on + strand at 773.151 kb on + strand at 773.151 kb on - strand at 773.161 kb on + strand at 773.161 kb on + strand at 773.169 kb on - strand at 773.179 kb on - strand at 773.184 kb on - strand at 773.184 kb on - strand at 773.189 kb on - strand at 773.194 kb on + strand at 773.194 kb on + strand at 773.213 kb on + strand at 773.218 kb on + strand at 773.221 kb on - strand at 773.221 kb on - strand at 773.248 kb on + strand at 773.248 kb on + strand at 773.249 kb on + strand at 773.249 kb on + strand at 773.249 kb on + strand at 773.256 kb on - strand at 773.256 kb on - strand at 773.256 kb on - strand at 773.257 kb on - strand at 773.257 kb on - strand at 773.257 kb on - strand at 773.257 kb on - strand at 773.271 kb on + strand at 773.278 kb on - strand at 773.334 kb on - strand at 773.367 kb on + strand at 773.373 kb on + strand at 773.640 kb on + strand, within CCNA_03931 at 773.732 kb on + strand at 773.732 kb on + strand at 773.732 kb on + strand at 773.742 kb on + strand at 773.750 kb on - strand at 773.757 kb on - strand at 773.757 kb on - strand at 773.778 kb on + strand, within CCNA_03932 at 773.821 kb on - strand, within CCNA_03932 at 773.823 kb on + strand, within CCNA_03932 at 773.829 kb on + strand, within CCNA_03932 at 773.837 kb on - strand, within CCNA_03932 at 773.865 kb on - strand, within CCNA_03932 at 773.890 kb on - strand, within CCNA_03932 at 773.909 kb on - strand, within CCNA_03932 at 773.909 kb on - strand, within CCNA_03932 at 773.924 kb on + strand, within CCNA_03932 at 773.936 kb on + strand, within CCNA_03932 at 773.944 kb on - strand, within CCNA_03932 at 773.954 kb on - strand, within CCNA_03932 at 773.964 kb on - strand at 774.038 kb on - strand at 774.038 kb on - strand at 774.060 kb on + strand, within CCNA_03933 at 774.065 kb on - strand, within CCNA_03933 at 774.065 kb on - strand, within CCNA_03933 at 774.090 kb on - strand, within CCNA_03933 at 774.109 kb on - strand, within CCNA_03933 at 774.115 kb on - strand, within CCNA_03933 at 774.119 kb on + strand, within CCNA_03933 at 774.123 kb on - strand, within CCNA_03933 at 774.181 kb on + strand, within CCNA_03933 at 774.188 kb on + strand, within CCNA_03933 at 774.192 kb on + strand, within CCNA_03933 at 774.200 kb on - strand, within CCNA_03933 at 774.204 kb on + strand, within CCNA_03933 at 774.209 kb on + strand, within CCNA_03933 at 774.280 kb on + strand at 774.285 kb on + strand at 774.288 kb on - strand at 774.299 kb on - strand at 774.301 kb on - strand at 774.309 kb on - strand at 774.337 kb on - strand at 774.360 kb on - strand at 774.424 kb on + strand at 774.427 kb on - strand at 774.446 kb on - strand at 774.467 kb on + strand at 774.552 kb on - strand, within CCNA_00719 at 774.553 kb on + strand, within CCNA_00719 at 774.575 kb on + strand, within CCNA_00719 at 774.583 kb on + strand, within CCNA_00719 at 774.618 kb on + strand, within CCNA_00719 at 774.618 kb on + strand, within CCNA_00719 at 774.619 kb on + strand, within CCNA_00719 at 774.635 kb on + strand, within CCNA_00719 at 774.643 kb on - strand, within CCNA_00719 at 774.643 kb on - strand, within CCNA_00719 at 774.665 kb on + strand, within CCNA_00719 at 774.685 kb on + strand, within CCNA_00719 at 774.706 kb on + strand, within CCNA_00719 at 774.714 kb on - strand, within CCNA_00719
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 772,455 - CCNA_00718 0.63 +1.2 772,455 - CCNA_00718 0.63 +0.2 772,468 + CCNA_00718 0.65 +1.1 772,468 + CCNA_00718 0.65 +0.7 772,468 + CCNA_00718 0.65 +0.2 772,476 - CCNA_00718 0.66 -0.5 772,476 - CCNA_00718 0.66 +0.1 772,476 - CCNA_00718 0.66 +0.2 772,476 - CCNA_00718 0.66 +0.0 772,476 - CCNA_00718 0.66 +1.0 772,488 - CCNA_00718 0.67 +0.2 772,523 - CCNA_00718 0.71 +0.4 772,582 - CCNA_00718 0.77 +0.1 772,596 + CCNA_00718 0.79 -3.7 772,698 - -0.0 772,698 - +0.4 772,724 + -0.8 772,745 - +0.8 772,756 - +0.4 772,787 + +0.2 772,787 + -1.8 772,802 + CCNA_03930 0.11 -1.2 772,803 - CCNA_03930 0.12 -0.1 772,805 - CCNA_03930 0.14 +1.3 772,820 + CCNA_03930 0.29 +1.6 772,828 - CCNA_03930 0.36 -0.2 772,847 + CCNA_03930 0.54 +1.1 772,852 + CCNA_03930 0.59 +0.4 772,860 - CCNA_03930 0.67 +0.2 772,866 + CCNA_03930 0.72 -0.7 772,869 - CCNA_03930 0.75 +0.1 772,897 - +1.0 772,945 + -0.1 772,968 + +0.5 772,975 + +0.4 772,985 + +0.4 772,986 + -0.3 772,991 + +1.3 773,027 + -0.8 773,030 - +0.1 773,060 - -0.4 773,080 + +0.2 773,094 + +0.7 773,101 - -0.2 773,102 - +0.6 773,120 + +0.8 773,151 + +1.3 773,151 - +0.2 773,161 + +1.0 773,161 + +0.3 773,169 - +0.8 773,179 - +0.3 773,184 - +0.0 773,184 - +0.8 773,189 - +0.3 773,194 + +1.6 773,194 + +0.2 773,213 + +1.9 773,218 + +0.0 773,221 - +0.2 773,221 - +0.0 773,248 + +0.4 773,248 + -0.9 773,249 + -0.8 773,249 + +0.5 773,249 + +0.3 773,256 - +0.2 773,256 - +0.3 773,256 - +0.3 773,257 - +0.6 773,257 - -0.1 773,257 - +0.4 773,257 - +0.4 773,271 + -0.4 773,278 - +0.2 773,334 - +0.5 773,367 + +1.4 773,373 + -0.8 773,640 + CCNA_03931 0.69 -0.8 773,732 + +0.0 773,732 + +1.2 773,732 + -0.3 773,742 + -1.9 773,750 - -0.7 773,757 - +0.5 773,757 - -0.0 773,778 + CCNA_03932 0.18 -1.5 773,821 - CCNA_03932 0.35 +0.6 773,823 + CCNA_03932 0.35 -1.1 773,829 + CCNA_03932 0.38 -1.3 773,837 - CCNA_03932 0.41 +0.5 773,865 - CCNA_03932 0.52 +0.3 773,890 - CCNA_03932 0.62 +0.4 773,909 - CCNA_03932 0.69 -0.2 773,909 - CCNA_03932 0.69 -0.2 773,924 + CCNA_03932 0.75 -1.0 773,936 + CCNA_03932 0.80 +0.5 773,944 - CCNA_03932 0.83 +0.6 773,954 - CCNA_03932 0.87 +0.3 773,964 - +0.5 774,038 - -1.4 774,038 - +1.1 774,060 + CCNA_03933 0.16 -0.4 774,065 - CCNA_03933 0.18 +0.6 774,065 - CCNA_03933 0.18 +0.3 774,090 - CCNA_03933 0.28 +0.3 774,109 - CCNA_03933 0.35 +1.3 774,115 - CCNA_03933 0.37 +0.7 774,119 + CCNA_03933 0.39 +0.7 774,123 - CCNA_03933 0.40 +0.6 774,181 + CCNA_03933 0.62 -0.8 774,188 + CCNA_03933 0.65 -0.3 774,192 + CCNA_03933 0.67 -0.1 774,200 - CCNA_03933 0.70 +0.9 774,204 + CCNA_03933 0.71 -0.9 774,209 + CCNA_03933 0.73 +0.1 774,280 + +0.2 774,285 + +1.9 774,288 - +0.7 774,299 - -0.5 774,301 - +0.2 774,309 - +0.2 774,337 - -0.1 774,360 - -0.7 774,424 + +0.2 774,427 - -0.4 774,446 - +1.6 774,467 + +0.8 774,552 - CCNA_00719 0.13 +0.3 774,553 + CCNA_00719 0.13 -0.1 774,575 + CCNA_00719 0.15 -0.1 774,583 + CCNA_00719 0.15 +0.0 774,618 + CCNA_00719 0.18 +1.2 774,618 + CCNA_00719 0.18 +0.0 774,619 + CCNA_00719 0.18 +1.0 774,635 + CCNA_00719 0.19 +0.2 774,643 - CCNA_00719 0.20 +1.3 774,643 - CCNA_00719 0.20 -1.7 774,665 + CCNA_00719 0.22 -0.4 774,685 + CCNA_00719 0.23 +0.3 774,706 + CCNA_00719 0.25 +0.8 774,714 - CCNA_00719 0.26 -0.6
Or see this region's nucleotide sequence