Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02378 and CCNA_02379 are separated by 69 nucleotides CCNA_02379 and CCNA_02380 are separated by 3 nucleotides CCNA_02380 and CCNA_02381 are separated by 177 nucleotides CCNA_02381 and CCNA_02382 are separated by 1 nucleotides
CCNA_02378: CCNA_02378 - SIMPL family protein, at 2,521,693 to 2,522,439
_02378
CCNA_02379: CCNA_02379 - hypothetical protein, at 2,522,509 to 2,522,988
_02379
CCNA_02380: CCNA_02380 - aspartate 1-decarboxylase, at 2,522,992 to 2,523,348
_02380
CCNA_02381: CCNA_02381 - hypothetical protein, at 2,523,526 to 2,524,176
_02381
CCNA_02382: CCNA_02382 - membrane peptidase, M23 family, at 2,524,178 to 2,525,074
_02382
Position (kb)
2522
2523
2524 Strain fitness (log2 ratio)
-1
0
1
2 at 2522.024 kb on + strand, within CCNA_02378 at 2522.024 kb on + strand, within CCNA_02378 at 2522.024 kb on + strand, within CCNA_02378 at 2522.050 kb on - strand, within CCNA_02378 at 2522.065 kb on + strand, within CCNA_02378 at 2522.066 kb on + strand, within CCNA_02378 at 2522.081 kb on + strand, within CCNA_02378 at 2522.123 kb on + strand, within CCNA_02378 at 2522.142 kb on + strand, within CCNA_02378 at 2522.165 kb on + strand, within CCNA_02378 at 2522.165 kb on + strand, within CCNA_02378 at 2522.165 kb on + strand, within CCNA_02378 at 2522.165 kb on + strand, within CCNA_02378 at 2522.208 kb on + strand, within CCNA_02378 at 2522.208 kb on + strand, within CCNA_02378 at 2522.216 kb on - strand, within CCNA_02378 at 2522.232 kb on + strand, within CCNA_02378 at 2522.232 kb on + strand, within CCNA_02378 at 2522.232 kb on + strand, within CCNA_02378 at 2522.240 kb on - strand, within CCNA_02378 at 2522.240 kb on - strand, within CCNA_02378 at 2522.245 kb on + strand, within CCNA_02378 at 2522.245 kb on + strand, within CCNA_02378 at 2522.245 kb on + strand, within CCNA_02378 at 2522.263 kb on + strand, within CCNA_02378 at 2522.263 kb on + strand, within CCNA_02378 at 2522.346 kb on + strand, within CCNA_02378 at 2522.532 kb on - strand at 2522.543 kb on - strand at 2522.615 kb on + strand, within CCNA_02379 at 2522.739 kb on - strand, within CCNA_02379 at 2522.789 kb on - strand, within CCNA_02379 at 2522.800 kb on + strand, within CCNA_02379 at 2522.838 kb on - strand, within CCNA_02379 at 2522.845 kb on + strand, within CCNA_02379 at 2522.845 kb on + strand, within CCNA_02379 at 2522.853 kb on - strand, within CCNA_02379 at 2522.853 kb on - strand, within CCNA_02379 at 2522.876 kb on + strand, within CCNA_02379 at 2522.878 kb on - strand, within CCNA_02379 at 2522.889 kb on - strand, within CCNA_02379 at 2522.894 kb on - strand, within CCNA_02379 at 2522.895 kb on + strand, within CCNA_02379 at 2522.903 kb on - strand, within CCNA_02379 at 2522.928 kb on + strand, within CCNA_02379 at 2522.978 kb on - strand at 2523.007 kb on - strand at 2523.180 kb on - strand, within CCNA_02380 at 2523.220 kb on - strand, within CCNA_02380 at 2523.334 kb on - strand at 2523.358 kb on - strand at 2523.384 kb on + strand at 2523.421 kb on + strand at 2523.447 kb on + strand at 2523.568 kb on + strand at 2523.590 kb on - strand at 2523.767 kb on + strand, within CCNA_02381 at 2523.827 kb on + strand, within CCNA_02381 at 2523.829 kb on + strand, within CCNA_02381 at 2523.852 kb on + strand, within CCNA_02381 at 2523.894 kb on - strand, within CCNA_02381 at 2523.965 kb on - strand, within CCNA_02381 at 2523.965 kb on - strand, within CCNA_02381 at 2523.993 kb on - strand, within CCNA_02381 at 2524.035 kb on + strand, within CCNA_02381 at 2524.035 kb on + strand, within CCNA_02381 at 2524.035 kb on + strand, within CCNA_02381 at 2524.035 kb on + strand, within CCNA_02381 at 2524.035 kb on + strand, within CCNA_02381 at 2524.035 kb on + strand, within CCNA_02381 at 2524.035 kb on + strand, within CCNA_02381 at 2524.043 kb on - strand, within CCNA_02381 at 2524.043 kb on - strand, within CCNA_02381 at 2524.043 kb on - strand, within CCNA_02381 at 2524.043 kb on - strand, within CCNA_02381 at 2524.094 kb on + strand, within CCNA_02381 at 2524.094 kb on + strand, within CCNA_02381 at 2524.102 kb on - strand, within CCNA_02381 at 2524.127 kb on - strand at 2524.137 kb on + strand at 2524.145 kb on - strand at 2524.145 kb on - strand at 2524.145 kb on - strand at 2524.187 kb on + strand at 2524.193 kb on - strand at 2524.204 kb on - strand at 2524.209 kb on + strand at 2524.342 kb on + strand, within CCNA_02382
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 2,522,024 + CCNA_02378 0.44 +0.9 2,522,024 + CCNA_02378 0.44 -0.2 2,522,024 + CCNA_02378 0.44 +1.0 2,522,050 - CCNA_02378 0.48 +0.0 2,522,065 + CCNA_02378 0.50 -0.5 2,522,066 + CCNA_02378 0.50 +0.4 2,522,081 + CCNA_02378 0.52 +0.4 2,522,123 + CCNA_02378 0.58 +0.5 2,522,142 + CCNA_02378 0.60 -1.1 2,522,165 + CCNA_02378 0.63 +0.8 2,522,165 + CCNA_02378 0.63 -0.1 2,522,165 + CCNA_02378 0.63 +0.3 2,522,165 + CCNA_02378 0.63 -1.5 2,522,208 + CCNA_02378 0.69 -0.6 2,522,208 + CCNA_02378 0.69 -0.7 2,522,216 - CCNA_02378 0.70 +0.3 2,522,232 + CCNA_02378 0.72 +1.1 2,522,232 + CCNA_02378 0.72 -0.1 2,522,232 + CCNA_02378 0.72 +0.2 2,522,240 - CCNA_02378 0.73 +2.5 2,522,240 - CCNA_02378 0.73 -0.1 2,522,245 + CCNA_02378 0.74 -0.0 2,522,245 + CCNA_02378 0.74 +0.8 2,522,245 + CCNA_02378 0.74 -0.1 2,522,263 + CCNA_02378 0.76 -1.2 2,522,263 + CCNA_02378 0.76 -0.1 2,522,346 + CCNA_02378 0.87 +0.2 2,522,532 - +0.6 2,522,543 - +0.1 2,522,615 + CCNA_02379 0.22 -0.4 2,522,739 - CCNA_02379 0.48 +0.4 2,522,789 - CCNA_02379 0.58 +0.6 2,522,800 + CCNA_02379 0.61 -0.6 2,522,838 - CCNA_02379 0.69 -0.2 2,522,845 + CCNA_02379 0.70 +1.5 2,522,845 + CCNA_02379 0.70 +0.9 2,522,853 - CCNA_02379 0.72 +1.0 2,522,853 - CCNA_02379 0.72 -0.1 2,522,876 + CCNA_02379 0.76 -0.7 2,522,878 - CCNA_02379 0.77 -0.5 2,522,889 - CCNA_02379 0.79 +0.4 2,522,894 - CCNA_02379 0.80 +1.4 2,522,895 + CCNA_02379 0.80 -0.3 2,522,903 - CCNA_02379 0.82 -0.6 2,522,928 + CCNA_02379 0.87 -0.5 2,522,978 - +0.9 2,523,007 - +0.5 2,523,180 - CCNA_02380 0.53 -0.9 2,523,220 - CCNA_02380 0.64 -0.7 2,523,334 - -0.4 2,523,358 - +0.8 2,523,384 + +1.5 2,523,421 + -0.7 2,523,447 + -1.3 2,523,568 + -1.5 2,523,590 - -0.3 2,523,767 + CCNA_02381 0.37 +0.3 2,523,827 + CCNA_02381 0.46 -0.4 2,523,829 + CCNA_02381 0.47 +0.4 2,523,852 + CCNA_02381 0.50 -1.5 2,523,894 - CCNA_02381 0.57 -0.9 2,523,965 - CCNA_02381 0.67 +0.2 2,523,965 - CCNA_02381 0.67 +0.1 2,523,993 - CCNA_02381 0.72 -0.7 2,524,035 + CCNA_02381 0.78 -0.4 2,524,035 + CCNA_02381 0.78 -0.5 2,524,035 + CCNA_02381 0.78 +0.1 2,524,035 + CCNA_02381 0.78 +1.1 2,524,035 + CCNA_02381 0.78 +0.3 2,524,035 + CCNA_02381 0.78 +0.3 2,524,035 + CCNA_02381 0.78 -0.1 2,524,043 - CCNA_02381 0.79 +0.2 2,524,043 - CCNA_02381 0.79 -1.0 2,524,043 - CCNA_02381 0.79 +0.9 2,524,043 - CCNA_02381 0.79 +0.5 2,524,094 + CCNA_02381 0.87 -1.2 2,524,094 + CCNA_02381 0.87 -0.1 2,524,102 - CCNA_02381 0.88 +1.1 2,524,127 - -0.4 2,524,137 + +0.1 2,524,145 - +0.2 2,524,145 - -0.1 2,524,145 - -0.7 2,524,187 + -1.6 2,524,193 - -0.3 2,524,204 - -0.7 2,524,209 + -0.8 2,524,342 + CCNA_02382 0.18 -0.5
Or see this region's nucleotide sequence