Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02271 and CCNA_02272 are separated by 68 nucleotides CCNA_02272 and CCNA_02273 are separated by 34 nucleotides CCNA_02273 and CCNA_02274 are separated by 125 nucleotides CCNA_02274 and CCNA_02275 overlap by 4 nucleotides
CCNA_02271: CCNA_02271 - stalk cross-band protein StpD, at 2,423,833 to 2,424,618
_02271
CCNA_02272: CCNA_02272 - hypothetical protein, at 2,424,687 to 2,424,929
_02272
CCNA_02273: CCNA_02273 - glutathione peroxidase, at 2,424,964 to 2,425,212
_02273
CCNA_02274: CCNA_02274 - EF-Hand domain protein, at 2,425,338 to 2,425,823
_02274
CCNA_02275: CCNA_02275 - ABC transporter, periplasmic component, at 2,425,820 to 2,426,605
_02275
Position (kb)
2424
2425
2426 Strain fitness (log2 ratio)
-2
-1
0
1 at 2424.008 kb on + strand, within CCNA_02271 at 2424.041 kb on + strand, within CCNA_02271 at 2424.041 kb on + strand, within CCNA_02271 at 2424.183 kb on - strand, within CCNA_02271 at 2424.229 kb on - strand, within CCNA_02271 at 2424.260 kb on + strand, within CCNA_02271 at 2424.262 kb on + strand, within CCNA_02271 at 2424.268 kb on - strand, within CCNA_02271 at 2424.269 kb on + strand, within CCNA_02271 at 2424.272 kb on + strand, within CCNA_02271 at 2424.280 kb on - strand, within CCNA_02271 at 2424.290 kb on - strand, within CCNA_02271 at 2424.290 kb on - strand, within CCNA_02271 at 2424.313 kb on + strand, within CCNA_02271 at 2424.370 kb on - strand, within CCNA_02271 at 2424.443 kb on + strand, within CCNA_02271 at 2424.577 kb on - strand at 2424.648 kb on + strand at 2424.747 kb on + strand, within CCNA_02272 at 2424.755 kb on - strand, within CCNA_02272 at 2424.805 kb on - strand, within CCNA_02272 at 2424.852 kb on + strand, within CCNA_02272 at 2424.904 kb on - strand, within CCNA_02272 at 2424.962 kb on + strand at 2425.006 kb on - strand, within CCNA_02273 at 2425.248 kb on + strand at 2425.343 kb on - strand at 2425.576 kb on + strand, within CCNA_02274 at 2425.576 kb on + strand, within CCNA_02274 at 2425.584 kb on - strand, within CCNA_02274 at 2425.584 kb on - strand, within CCNA_02274 at 2425.649 kb on - strand, within CCNA_02274 at 2425.740 kb on + strand, within CCNA_02274 at 2425.746 kb on + strand, within CCNA_02274 at 2425.772 kb on - strand, within CCNA_02274 at 2425.782 kb on + strand at 2425.782 kb on + strand at 2425.782 kb on + strand at 2425.790 kb on + strand at 2425.790 kb on - strand at 2425.838 kb on + strand at 2425.856 kb on + strand at 2426.013 kb on - strand, within CCNA_02275 at 2426.015 kb on + strand, within CCNA_02275 at 2426.020 kb on + strand, within CCNA_02275 at 2426.075 kb on + strand, within CCNA_02275 at 2426.120 kb on + strand, within CCNA_02275 at 2426.120 kb on - strand, within CCNA_02275 at 2426.127 kb on + strand, within CCNA_02275 at 2426.128 kb on - strand, within CCNA_02275 at 2426.191 kb on + strand, within CCNA_02275 at 2426.192 kb on + strand, within CCNA_02275 at 2426.193 kb on + strand, within CCNA_02275 at 2426.195 kb on - strand, within CCNA_02275 at 2426.199 kb on - strand, within CCNA_02275 at 2426.199 kb on - strand, within CCNA_02275 at 2426.199 kb on - strand, within CCNA_02275
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 2,424,008 + CCNA_02271 0.22 -1.5 2,424,041 + CCNA_02271 0.26 -0.1 2,424,041 + CCNA_02271 0.26 -0.1 2,424,183 - CCNA_02271 0.45 -0.9 2,424,229 - CCNA_02271 0.50 -0.3 2,424,260 + CCNA_02271 0.54 -1.3 2,424,262 + CCNA_02271 0.55 -0.1 2,424,268 - CCNA_02271 0.55 +0.6 2,424,269 + CCNA_02271 0.55 +0.0 2,424,272 + CCNA_02271 0.56 +0.4 2,424,280 - CCNA_02271 0.57 -0.1 2,424,290 - CCNA_02271 0.58 -1.5 2,424,290 - CCNA_02271 0.58 +0.8 2,424,313 + CCNA_02271 0.61 +1.4 2,424,370 - CCNA_02271 0.68 +0.3 2,424,443 + CCNA_02271 0.78 +0.1 2,424,577 - +1.0 2,424,648 + -1.8 2,424,747 + CCNA_02272 0.25 -0.1 2,424,755 - CCNA_02272 0.28 -0.0 2,424,805 - CCNA_02272 0.49 +0.1 2,424,852 + CCNA_02272 0.68 -1.1 2,424,904 - CCNA_02272 0.89 +1.2 2,424,962 + -1.3 2,425,006 - CCNA_02273 0.17 +0.5 2,425,248 + +0.4 2,425,343 - +0.3 2,425,576 + CCNA_02274 0.49 -1.3 2,425,576 + CCNA_02274 0.49 -0.9 2,425,584 - CCNA_02274 0.51 +0.2 2,425,584 - CCNA_02274 0.51 +0.3 2,425,649 - CCNA_02274 0.64 +0.6 2,425,740 + CCNA_02274 0.83 -1.2 2,425,746 + CCNA_02274 0.84 -1.3 2,425,772 - CCNA_02274 0.89 +1.3 2,425,782 + -0.3 2,425,782 + -2.1 2,425,782 + +0.1 2,425,790 + -1.9 2,425,790 - +0.2 2,425,838 + -1.6 2,425,856 + -2.6 2,426,013 - CCNA_02275 0.25 +0.2 2,426,015 + CCNA_02275 0.25 -1.1 2,426,020 + CCNA_02275 0.25 -1.9 2,426,075 + CCNA_02275 0.32 -1.9 2,426,120 + CCNA_02275 0.38 -1.1 2,426,120 - CCNA_02275 0.38 +0.3 2,426,127 + CCNA_02275 0.39 +0.7 2,426,128 - CCNA_02275 0.39 +0.3 2,426,191 + CCNA_02275 0.47 -1.3 2,426,192 + CCNA_02275 0.47 -0.1 2,426,193 + CCNA_02275 0.47 -1.4 2,426,195 - CCNA_02275 0.48 +0.1 2,426,199 - CCNA_02275 0.48 +0.3 2,426,199 - CCNA_02275 0.48 +0.9 2,426,199 - CCNA_02275 0.48 +1.5
Or see this region's nucleotide sequence