Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01084 and CCNA_01085 overlap by 4 nucleotides CCNA_01085 and CCNA_01086 are separated by 192 nucleotides
CCNA_01084: CCNA_01084 - hypothetical protein, at 1,188,873 to 1,190,108
_01084
CCNA_01085: CCNA_01085 - DNA-cytosine methyltransferase, at 1,190,105 to 1,191,112
_01085
CCNA_01086: CCNA_01086 - GTP-binding protein lepA, at 1,191,305 to 1,193,113
_01086
Position (kb)
1190
1191
1192 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1189.247 kb on + strand, within CCNA_01084 at 1189.257 kb on - strand, within CCNA_01084 at 1189.324 kb on + strand, within CCNA_01084 at 1189.351 kb on - strand, within CCNA_01084 at 1189.374 kb on - strand, within CCNA_01084 at 1189.502 kb on + strand, within CCNA_01084 at 1189.522 kb on + strand, within CCNA_01084 at 1189.530 kb on - strand, within CCNA_01084 at 1189.533 kb on + strand, within CCNA_01084 at 1189.534 kb on + strand, within CCNA_01084 at 1189.542 kb on - strand, within CCNA_01084 at 1189.542 kb on - strand, within CCNA_01084 at 1189.542 kb on - strand, within CCNA_01084 at 1189.542 kb on - strand, within CCNA_01084 at 1189.601 kb on - strand, within CCNA_01084 at 1189.631 kb on + strand, within CCNA_01084 at 1189.639 kb on - strand, within CCNA_01084 at 1189.683 kb on + strand, within CCNA_01084 at 1189.793 kb on + strand, within CCNA_01084 at 1189.863 kb on + strand, within CCNA_01084 at 1189.871 kb on + strand, within CCNA_01084 at 1189.922 kb on - strand, within CCNA_01084 at 1189.970 kb on + strand, within CCNA_01084 at 1190.035 kb on + strand at 1190.074 kb on - strand at 1190.097 kb on + strand at 1190.272 kb on + strand, within CCNA_01085 at 1190.325 kb on - strand, within CCNA_01085 at 1190.325 kb on - strand, within CCNA_01085 at 1190.325 kb on - strand, within CCNA_01085 at 1190.580 kb on - strand, within CCNA_01085 at 1190.581 kb on + strand, within CCNA_01085 at 1190.657 kb on - strand, within CCNA_01085 at 1190.679 kb on + strand, within CCNA_01085 at 1190.780 kb on + strand, within CCNA_01085 at 1190.808 kb on + strand, within CCNA_01085 at 1190.811 kb on - strand, within CCNA_01085 at 1190.893 kb on + strand, within CCNA_01085 at 1190.901 kb on - strand, within CCNA_01085 at 1190.997 kb on - strand, within CCNA_01085 at 1191.051 kb on - strand at 1191.087 kb on + strand at 1191.139 kb on - strand at 1191.139 kb on - strand at 1191.165 kb on - strand at 1191.210 kb on + strand at 1191.284 kb on + strand at 1191.318 kb on + strand at 1191.351 kb on + strand at 1191.401 kb on + strand at 1191.435 kb on + strand at 1191.458 kb on + strand at 1191.468 kb on + strand at 1191.530 kb on + strand, within CCNA_01086 at 1191.558 kb on - strand, within CCNA_01086 at 1191.606 kb on + strand, within CCNA_01086 at 1191.614 kb on - strand, within CCNA_01086 at 1191.614 kb on - strand, within CCNA_01086 at 1191.614 kb on - strand, within CCNA_01086 at 1191.701 kb on + strand, within CCNA_01086 at 1191.774 kb on - strand, within CCNA_01086 at 1191.812 kb on - strand, within CCNA_01086 at 1191.868 kb on - strand, within CCNA_01086 at 1191.896 kb on - strand, within CCNA_01086 at 1191.929 kb on - strand, within CCNA_01086 at 1191.944 kb on + strand, within CCNA_01086 at 1191.949 kb on + strand, within CCNA_01086 at 1191.973 kb on + strand, within CCNA_01086 at 1191.981 kb on - strand, within CCNA_01086 at 1192.102 kb on - strand, within CCNA_01086 at 1192.112 kb on - strand, within CCNA_01086
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 1,189,247 + CCNA_01084 0.30 +1.2 1,189,257 - CCNA_01084 0.31 +0.5 1,189,324 + CCNA_01084 0.36 +0.5 1,189,351 - CCNA_01084 0.39 -0.2 1,189,374 - CCNA_01084 0.41 +0.1 1,189,502 + CCNA_01084 0.51 +0.3 1,189,522 + CCNA_01084 0.53 -0.7 1,189,530 - CCNA_01084 0.53 -0.6 1,189,533 + CCNA_01084 0.53 +0.0 1,189,534 + CCNA_01084 0.53 +0.9 1,189,542 - CCNA_01084 0.54 +0.1 1,189,542 - CCNA_01084 0.54 +0.5 1,189,542 - CCNA_01084 0.54 +0.3 1,189,542 - CCNA_01084 0.54 -1.2 1,189,601 - CCNA_01084 0.59 +0.2 1,189,631 + CCNA_01084 0.61 +0.1 1,189,639 - CCNA_01084 0.62 +0.6 1,189,683 + CCNA_01084 0.66 +0.7 1,189,793 + CCNA_01084 0.74 +0.7 1,189,863 + CCNA_01084 0.80 -0.3 1,189,871 + CCNA_01084 0.81 +0.1 1,189,922 - CCNA_01084 0.85 -1.1 1,189,970 + CCNA_01084 0.89 +0.7 1,190,035 + -0.1 1,190,074 - +0.0 1,190,097 + -1.6 1,190,272 + CCNA_01085 0.17 -0.4 1,190,325 - CCNA_01085 0.22 -0.6 1,190,325 - CCNA_01085 0.22 -0.2 1,190,325 - CCNA_01085 0.22 -0.2 1,190,580 - CCNA_01085 0.47 -0.6 1,190,581 + CCNA_01085 0.47 +0.3 1,190,657 - CCNA_01085 0.55 -1.8 1,190,679 + CCNA_01085 0.57 +0.4 1,190,780 + CCNA_01085 0.67 +0.2 1,190,808 + CCNA_01085 0.70 -1.0 1,190,811 - CCNA_01085 0.70 -0.4 1,190,893 + CCNA_01085 0.78 +0.3 1,190,901 - CCNA_01085 0.79 -0.0 1,190,997 - CCNA_01085 0.88 -0.2 1,191,051 - -0.9 1,191,087 + -1.7 1,191,139 - +0.6 1,191,139 - +0.4 1,191,165 - -0.0 1,191,210 + -0.4 1,191,284 + -0.7 1,191,318 + +0.2 1,191,351 + -0.7 1,191,401 + +0.3 1,191,435 + -0.4 1,191,458 + +1.0 1,191,468 + -1.2 1,191,530 + CCNA_01086 0.12 -0.4 1,191,558 - CCNA_01086 0.14 +0.0 1,191,606 + CCNA_01086 0.17 +0.4 1,191,614 - CCNA_01086 0.17 -1.1 1,191,614 - CCNA_01086 0.17 -1.5 1,191,614 - CCNA_01086 0.17 +0.8 1,191,701 + CCNA_01086 0.22 -0.4 1,191,774 - CCNA_01086 0.26 -1.6 1,191,812 - CCNA_01086 0.28 -0.4 1,191,868 - CCNA_01086 0.31 +0.4 1,191,896 - CCNA_01086 0.33 -0.2 1,191,929 - CCNA_01086 0.34 -0.3 1,191,944 + CCNA_01086 0.35 -0.1 1,191,949 + CCNA_01086 0.36 -0.2 1,191,973 + CCNA_01086 0.37 -0.9 1,191,981 - CCNA_01086 0.37 +1.8 1,192,102 - CCNA_01086 0.44 -0.6 1,192,112 - CCNA_01086 0.45 +0.4
Or see this region's nucleotide sequence