Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00466 and CCNA_00467 are separated by 3 nucleotides CCNA_00467 and CCNA_00468 are separated by 109 nucleotides CCNA_00468 and CCNA_00469 are separated by 297 nucleotides
CCNA_00466: CCNA_00466 - glycosyltransferase, at 479,191 to 480,435
_00466
CCNA_00467: CCNA_00467 - oligosaccharide translocase/flippase, at 480,439 to 481,710
_00467
CCNA_00468: CCNA_00468 - UDP glucuronic acid epimerase-related protein, at 481,820 to 481,933
_00468
CCNA_00469: CCNA_00469 - GT1-family glycosyltransferase, at 482,231 to 483,454
_00469
Position (kb) 480
481
482 Strain fitness (log2 ratio) -2
-1
0
1 at 480.434 kb on + strand at 481.657 kb on + strand at 481.664 kb on + strand at 481.664 kb on + strand at 481.664 kb on + strand at 481.664 kb on + strand at 481.672 kb on - strand at 481.672 kb on - strand at 481.672 kb on - strand at 481.672 kb on - strand at 481.690 kb on + strand at 481.704 kb on + strand at 481.712 kb on - strand at 481.712 kb on - strand at 481.712 kb on - strand at 481.765 kb on - strand at 481.796 kb on + strand at 481.804 kb on - strand at 481.804 kb on - strand at 481.833 kb on - strand, within CCNA_00468 at 481.842 kb on + strand, within CCNA_00468 at 481.850 kb on - strand, within CCNA_00468 at 481.852 kb on + strand, within CCNA_00468 at 481.880 kb on + strand, within CCNA_00468 at 481.944 kb on - strand at 481.982 kb on - strand at 481.990 kb on - strand at 482.004 kb on - strand at 482.004 kb on - strand at 482.004 kb on - strand at 482.026 kb on + strand at 482.066 kb on - strand at 482.071 kb on - strand at 482.103 kb on + strand at 482.103 kb on + strand at 482.103 kb on + strand at 482.103 kb on + strand at 482.111 kb on - strand at 482.111 kb on - strand at 482.111 kb on - strand at 482.111 kb on - strand at 482.111 kb on - strand at 482.127 kb on - strand at 482.158 kb on + strand at 482.158 kb on + strand at 482.166 kb on - strand at 482.166 kb on - strand at 482.209 kb on - strand at 482.224 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 480,434 + +0.1 481,657 + -0.6 481,664 + +0.2 481,664 + +0.9 481,664 + +0.2 481,664 + -0.0 481,672 - +0.2 481,672 - -0.6 481,672 - +0.1 481,672 - -0.4 481,690 + +0.3 481,704 + +0.2 481,712 - -0.1 481,712 - +0.9 481,712 - -0.1 481,765 - -0.6 481,796 + +0.4 481,804 - +0.1 481,804 - +0.3 481,833 - CCNA_00468 0.11 +0.5 481,842 + CCNA_00468 0.19 -0.4 481,850 - CCNA_00468 0.26 -1.1 481,852 + CCNA_00468 0.28 -1.0 481,880 + CCNA_00468 0.53 +0.3 481,944 - +0.2 481,982 - -2.0 481,990 - -0.1 482,004 - +1.3 482,004 - +0.0 482,004 - +0.4 482,026 + -0.5 482,066 - +0.4 482,071 - -0.5 482,103 + +1.1 482,103 + -0.9 482,103 + +0.5 482,103 + +0.0 482,111 - +0.4 482,111 - +0.8 482,111 - -0.3 482,111 - +0.4 482,111 - +1.7 482,127 - +0.4 482,158 + +0.4 482,158 + -0.5 482,166 - +0.4 482,166 - +0.3 482,209 - -0.0 482,224 + -1.9
Or see this region's nucleotide sequence