Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00224 and CCNA_00225 are separated by 330 nucleotides CCNA_00225 and CCNA_00226 are separated by 100 nucleotides CCNA_00226 and CCNA_00227 are separated by 57 nucleotides CCNA_00227 and CCNA_00228 overlap by 1 nucleotides
CCNA_00224: CCNA_00224 - TonB-dependent receptor protein, at 240,648 to 242,693
_00224
CCNA_00225: CCNA_00225 - hypothetical protein, at 243,024 to 243,056
_00225
CCNA_00226: CCNA_00226 - hypothetical protein, at 243,157 to 243,432
_00226
CCNA_00227: CCNA_00227 - methyltransferase, at 243,490 to 244,056
_00227
CCNA_00228: CCNA_00228 - putative membrane spanning protein, at 244,056 to 244,670
_00228
Position (kb) 243
244 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 242.025 kb on - strand, within CCNA_00224 at 242.092 kb on + strand, within CCNA_00224 at 242.095 kb on - strand, within CCNA_00224 at 242.122 kb on + strand, within CCNA_00224 at 242.130 kb on - strand, within CCNA_00224 at 242.172 kb on - strand, within CCNA_00224 at 242.216 kb on + strand, within CCNA_00224 at 242.216 kb on + strand, within CCNA_00224 at 242.216 kb on + strand, within CCNA_00224 at 242.224 kb on - strand, within CCNA_00224 at 242.224 kb on - strand, within CCNA_00224 at 242.229 kb on + strand, within CCNA_00224 at 242.285 kb on + strand, within CCNA_00224 at 242.285 kb on - strand, within CCNA_00224 at 242.299 kb on - strand, within CCNA_00224 at 242.299 kb on - strand, within CCNA_00224 at 242.343 kb on + strand, within CCNA_00224 at 242.365 kb on + strand, within CCNA_00224 at 242.390 kb on - strand, within CCNA_00224 at 242.409 kb on + strand, within CCNA_00224 at 242.428 kb on - strand, within CCNA_00224 at 242.438 kb on + strand, within CCNA_00224 at 242.445 kb on + strand, within CCNA_00224 at 242.473 kb on - strand, within CCNA_00224 at 242.481 kb on + strand, within CCNA_00224 at 242.489 kb on - strand at 242.489 kb on - strand at 242.489 kb on - strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.585 kb on - strand at 242.587 kb on + strand at 242.643 kb on + strand at 242.643 kb on + strand at 242.667 kb on + strand at 242.687 kb on - strand at 242.699 kb on + strand at 242.701 kb on + strand at 242.709 kb on - strand at 242.715 kb on + strand at 242.715 kb on + strand at 242.715 kb on + strand at 242.715 kb on + strand at 242.892 kb on + strand at 242.894 kb on + strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.942 kb on + strand at 242.965 kb on + strand at 242.971 kb on + strand at 242.973 kb on - strand at 243.010 kb on - strand at 243.015 kb on - strand at 243.051 kb on + strand, within CCNA_00225 at 243.057 kb on - strand at 243.083 kb on + strand at 243.091 kb on - strand at 243.116 kb on + strand at 243.154 kb on + strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.172 kb on - strand at 243.177 kb on + strand at 243.198 kb on + strand, within CCNA_00226 at 243.198 kb on + strand, within CCNA_00226 at 243.198 kb on + strand, within CCNA_00226 at 243.206 kb on - strand, within CCNA_00226 at 243.206 kb on - strand, within CCNA_00226 at 243.213 kb on + strand, within CCNA_00226 at 243.275 kb on + strand, within CCNA_00226 at 243.288 kb on - strand, within CCNA_00226 at 243.304 kb on + strand, within CCNA_00226 at 243.305 kb on + strand, within CCNA_00226 at 243.324 kb on + strand, within CCNA_00226 at 243.379 kb on - strand, within CCNA_00226 at 243.408 kb on - strand at 243.449 kb on + strand at 243.460 kb on - strand at 243.462 kb on + strand at 243.495 kb on - strand at 243.544 kb on + strand at 243.644 kb on + strand, within CCNA_00227 at 243.658 kb on + strand, within CCNA_00227 at 243.705 kb on - strand, within CCNA_00227 at 243.718 kb on + strand, within CCNA_00227 at 243.726 kb on - strand, within CCNA_00227 at 243.737 kb on - strand, within CCNA_00227 at 243.737 kb on - strand, within CCNA_00227 at 243.867 kb on + strand, within CCNA_00227 at 243.977 kb on - strand, within CCNA_00227 at 243.977 kb on - strand, within CCNA_00227 at 243.979 kb on + strand, within CCNA_00227 at 243.983 kb on + strand, within CCNA_00227 at 244.016 kb on + strand at 244.016 kb on + strand at 244.016 kb on + strand at 244.024 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 242,025 - CCNA_00224 0.67 -1.6 242,092 + CCNA_00224 0.71 +0.3 242,095 - CCNA_00224 0.71 +0.1 242,122 + CCNA_00224 0.72 +0.4 242,130 - CCNA_00224 0.72 -0.2 242,172 - CCNA_00224 0.74 +0.8 242,216 + CCNA_00224 0.77 +0.6 242,216 + CCNA_00224 0.77 -0.5 242,216 + CCNA_00224 0.77 -0.6 242,224 - CCNA_00224 0.77 +0.4 242,224 - CCNA_00224 0.77 -1.9 242,229 + CCNA_00224 0.77 -1.6 242,285 + CCNA_00224 0.80 -1.1 242,285 - CCNA_00224 0.80 +0.9 242,299 - CCNA_00224 0.81 +0.3 242,299 - CCNA_00224 0.81 +0.0 242,343 + CCNA_00224 0.83 -0.2 242,365 + CCNA_00224 0.84 -0.5 242,390 - CCNA_00224 0.85 +0.6 242,409 + CCNA_00224 0.86 +2.3 242,428 - CCNA_00224 0.87 -0.2 242,438 + CCNA_00224 0.87 -1.0 242,445 + CCNA_00224 0.88 -0.6 242,473 - CCNA_00224 0.89 +0.3 242,481 + CCNA_00224 0.90 -2.8 242,489 - +0.2 242,489 - -0.1 242,489 - +0.0 242,527 + -0.1 242,527 + -0.6 242,527 + -0.5 242,535 - -0.6 242,535 - +0.2 242,535 - -1.5 242,585 - +0.5 242,587 + -0.5 242,643 + -0.5 242,643 + -0.5 242,667 + -1.2 242,687 - +0.5 242,699 + +1.0 242,701 + +1.4 242,709 - -0.0 242,715 + -0.4 242,715 + +0.4 242,715 + -0.7 242,715 + -1.5 242,892 + -0.4 242,894 + +1.2 242,900 - +0.4 242,900 - +0.0 242,900 - +0.2 242,900 - -0.3 242,900 - -0.1 242,900 - -0.0 242,900 - +0.6 242,900 - -0.3 242,900 - -0.0 242,900 - +0.6 242,900 - +0.2 242,942 + -0.3 242,965 + -0.2 242,971 + +0.4 242,973 - -0.1 243,010 - -0.2 243,015 - -0.3 243,051 + CCNA_00225 0.82 -1.1 243,057 - +0.4 243,083 + -0.2 243,091 - +0.1 243,116 + -1.5 243,154 + -1.2 243,162 - +1.0 243,162 - +0.4 243,172 - -1.0 243,177 + +0.4 243,198 + CCNA_00226 0.15 +0.0 243,198 + CCNA_00226 0.15 +0.2 243,198 + CCNA_00226 0.15 +1.2 243,206 - CCNA_00226 0.18 -0.2 243,206 - CCNA_00226 0.18 +0.7 243,213 + CCNA_00226 0.20 +0.5 243,275 + CCNA_00226 0.43 -1.1 243,288 - CCNA_00226 0.47 -0.8 243,304 + CCNA_00226 0.53 -0.1 243,305 + CCNA_00226 0.54 -0.0 243,324 + CCNA_00226 0.61 +0.4 243,379 - CCNA_00226 0.80 -0.2 243,408 - -0.2 243,449 + -0.5 243,460 - -0.3 243,462 + +0.6 243,495 - +0.3 243,544 + -2.8 243,644 + CCNA_00227 0.27 -0.4 243,658 + CCNA_00227 0.30 -0.3 243,705 - CCNA_00227 0.38 +0.1 243,718 + CCNA_00227 0.40 -0.5 243,726 - CCNA_00227 0.42 -0.7 243,737 - CCNA_00227 0.44 -0.2 243,737 - CCNA_00227 0.44 -0.3 243,867 + CCNA_00227 0.66 -0.8 243,977 - CCNA_00227 0.86 -0.3 243,977 - CCNA_00227 0.86 +0.8 243,979 + CCNA_00227 0.86 +0.7 243,983 + CCNA_00227 0.87 +0.4 244,016 + +0.1 244,016 + -0.9 244,016 + -0.8 244,024 - +0.3
Or see this region's nucleotide sequence