Strain Fitness in Caulobacter crescentus NA1000 Δfur around CCNA_00202

Experiment: PYE with CHIR-090 4 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00200 and CCNA_00201 are separated by 162 nucleotidesCCNA_00201 and CCNA_00202 are separated by 116 nucleotidesCCNA_00202 and CCNA_00203 are separated by 57 nucleotides CCNA_00200: CCNA_00200 - 2OG-Fe(II) oxygenase superfamily protein, at 214,320 to 215,255 _00200 CCNA_00201: CCNA_00201 - outer membrane protein, at 215,418 to 216,098 _00201 CCNA_00202: CCNA_00202 - DesA-family fatty acid desaturase, at 216,215 to 217,270 _00202 CCNA_00203: CCNA_00203 - sterol desaturase family protein, at 217,328 to 218,332 _00203 Position (kb) 216 217 218Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 215.274 kb on + strandat 215.291 kb on - strandat 215.336 kb on + strandat 215.350 kb on + strandat 215.364 kb on - strandat 215.382 kb on + strandat 215.408 kb on + strandat 215.416 kb on - strandat 215.454 kb on + strandat 215.454 kb on + strandat 215.473 kb on - strandat 215.509 kb on - strand, within CCNA_00201at 215.509 kb on - strand, within CCNA_00201at 215.541 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.568 kb on + strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.576 kb on - strand, within CCNA_00201at 215.581 kb on - strand, within CCNA_00201at 215.595 kb on - strand, within CCNA_00201at 215.642 kb on - strand, within CCNA_00201at 215.677 kb on - strand, within CCNA_00201at 215.704 kb on + strand, within CCNA_00201at 215.756 kb on + strand, within CCNA_00201at 215.759 kb on - strand, within CCNA_00201at 215.764 kb on - strand, within CCNA_00201at 215.791 kb on + strand, within CCNA_00201at 215.796 kb on + strand, within CCNA_00201at 215.816 kb on - strand, within CCNA_00201at 215.816 kb on - strand, within CCNA_00201at 215.821 kb on - strand, within CCNA_00201at 215.916 kb on - strand, within CCNA_00201at 215.922 kb on + strand, within CCNA_00201at 215.922 kb on + strand, within CCNA_00201at 215.930 kb on - strand, within CCNA_00201at 215.979 kb on + strand, within CCNA_00201at 216.021 kb on - strand, within CCNA_00201at 216.035 kb on - strandat 216.043 kb on - strandat 216.054 kb on + strandat 216.062 kb on - strandat 216.087 kb on + strandat 216.087 kb on + strandat 216.092 kb on + strandat 216.094 kb on + strandat 216.143 kb on - strandat 216.168 kb on + strandat 216.187 kb on + strandat 216.195 kb on - strandat 216.197 kb on - strandat 216.207 kb on + strandat 216.210 kb on - strandat 216.234 kb on + strandat 216.253 kb on + strandat 216.255 kb on + strandat 216.281 kb on + strandat 216.301 kb on - strandat 216.306 kb on - strandat 216.334 kb on - strand, within CCNA_00202at 216.339 kb on - strand, within CCNA_00202at 216.376 kb on - strand, within CCNA_00202at 216.380 kb on + strand, within CCNA_00202at 216.383 kb on - strand, within CCNA_00202at 216.388 kb on - strand, within CCNA_00202at 216.395 kb on + strand, within CCNA_00202at 216.446 kb on + strand, within CCNA_00202at 216.461 kb on + strand, within CCNA_00202at 216.502 kb on - strand, within CCNA_00202at 216.529 kb on + strand, within CCNA_00202at 216.532 kb on + strand, within CCNA_00202at 216.540 kb on - strand, within CCNA_00202at 216.557 kb on - strand, within CCNA_00202at 216.559 kb on + strand, within CCNA_00202at 216.582 kb on + strand, within CCNA_00202at 216.590 kb on - strand, within CCNA_00202at 216.595 kb on - strand, within CCNA_00202at 216.607 kb on + strand, within CCNA_00202at 216.622 kb on + strand, within CCNA_00202at 216.669 kb on + strand, within CCNA_00202at 216.672 kb on - strand, within CCNA_00202at 216.674 kb on + strand, within CCNA_00202at 216.682 kb on - strand, within CCNA_00202at 216.682 kb on - strand, within CCNA_00202at 216.738 kb on + strand, within CCNA_00202at 216.740 kb on + strand, within CCNA_00202at 216.785 kb on + strand, within CCNA_00202at 216.785 kb on + strand, within CCNA_00202at 216.785 kb on + strand, within CCNA_00202at 216.785 kb on + strand, within CCNA_00202at 216.793 kb on - strand, within CCNA_00202at 216.793 kb on - strand, within CCNA_00202at 216.793 kb on - strand, within CCNA_00202at 216.793 kb on - strand, within CCNA_00202at 216.804 kb on + strand, within CCNA_00202at 216.805 kb on + strand, within CCNA_00202at 216.806 kb on - strand, within CCNA_00202at 216.840 kb on + strand, within CCNA_00202at 216.848 kb on - strand, within CCNA_00202at 216.860 kb on + strand, within CCNA_00202at 216.860 kb on + strand, within CCNA_00202at 216.866 kb on + strand, within CCNA_00202at 216.929 kb on + strand, within CCNA_00202at 216.956 kb on - strand, within CCNA_00202at 216.970 kb on - strand, within CCNA_00202at 216.990 kb on + strand, within CCNA_00202at 217.054 kb on + strand, within CCNA_00202at 217.072 kb on + strand, within CCNA_00202at 217.082 kb on - strand, within CCNA_00202at 217.084 kb on + strand, within CCNA_00202at 217.091 kb on - strand, within CCNA_00202at 217.121 kb on + strand, within CCNA_00202at 217.131 kb on + strand, within CCNA_00202at 217.155 kb on + strand, within CCNA_00202at 217.163 kb on - strand, within CCNA_00202at 217.170 kb on + strandat 217.176 kb on - strandat 217.188 kb on - strandat 217.222 kb on - strandat 217.327 kb on + strandat 217.333 kb on - strandat 217.342 kb on + strandat 217.346 kb on + strandat 217.385 kb on + strandat 217.390 kb on + strandat 217.403 kb on - strandat 217.420 kb on - strandat 217.420 kb on - strandat 217.435 kb on + strand, within CCNA_00203at 217.437 kb on - strand, within CCNA_00203at 217.480 kb on - strand, within CCNA_00203at 217.480 kb on - strand, within CCNA_00203at 217.480 kb on - strand, within CCNA_00203at 217.566 kb on + strand, within CCNA_00203at 217.571 kb on + strand, within CCNA_00203at 217.630 kb on + strand, within CCNA_00203at 217.632 kb on + strand, within CCNA_00203at 217.633 kb on - strand, within CCNA_00203at 217.638 kb on - strand, within CCNA_00203at 217.655 kb on + strand, within CCNA_00203at 217.655 kb on + strand, within CCNA_00203at 217.656 kb on - strand, within CCNA_00203at 217.656 kb on - strand, within CCNA_00203at 217.660 kb on + strand, within CCNA_00203at 217.668 kb on + strand, within CCNA_00203at 217.681 kb on + strand, within CCNA_00203at 217.689 kb on - strand, within CCNA_00203at 217.707 kb on + strand, within CCNA_00203at 217.707 kb on + strand, within CCNA_00203at 217.709 kb on - strand, within CCNA_00203at 217.737 kb on - strand, within CCNA_00203at 217.787 kb on - strand, within CCNA_00203at 217.798 kb on + strand, within CCNA_00203at 217.798 kb on + strand, within CCNA_00203at 217.826 kb on + strand, within CCNA_00203at 217.826 kb on + strand, within CCNA_00203at 217.826 kb on + strand, within CCNA_00203at 217.826 kb on + strand, within CCNA_00203at 217.826 kb on + strand, within CCNA_00203at 217.827 kb on - strand, within CCNA_00203at 217.827 kb on - strand, within CCNA_00203at 217.834 kb on - strand, within CCNA_00203at 217.852 kb on - strand, within CCNA_00203at 217.863 kb on - strand, within CCNA_00203at 217.864 kb on + strand, within CCNA_00203at 217.886 kb on + strand, within CCNA_00203at 217.888 kb on - strand, within CCNA_00203at 217.905 kb on + strand, within CCNA_00203at 217.910 kb on + strand, within CCNA_00203at 217.927 kb on + strand, within CCNA_00203at 217.927 kb on + strand, within CCNA_00203at 217.937 kb on + strand, within CCNA_00203at 217.937 kb on + strand, within CCNA_00203at 217.937 kb on + strand, within CCNA_00203at 217.942 kb on + strand, within CCNA_00203at 217.945 kb on - strand, within CCNA_00203at 217.945 kb on - strand, within CCNA_00203at 217.961 kb on + strand, within CCNA_00203at 217.973 kb on + strand, within CCNA_00203at 218.080 kb on + strand, within CCNA_00203at 218.080 kb on + strand, within CCNA_00203at 218.085 kb on + strand, within CCNA_00203at 218.085 kb on + strand, within CCNA_00203at 218.085 kb on + strand, within CCNA_00203at 218.088 kb on + strand, within CCNA_00203at 218.088 kb on - strand, within CCNA_00203at 218.093 kb on - strand, within CCNA_00203at 218.099 kb on - strand, within CCNA_00203at 218.115 kb on - strand, within CCNA_00203at 218.120 kb on - strand, within CCNA_00203at 218.138 kb on + strand, within CCNA_00203at 218.141 kb on - strand, within CCNA_00203at 218.173 kb on + strand, within CCNA_00203at 218.181 kb on - strand, within CCNA_00203at 218.183 kb on + strand, within CCNA_00203at 218.218 kb on - strand, within CCNA_00203at 218.259 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL
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215,274 + +0.4
215,291 - +0.7
215,336 + -1.0
215,350 + -0.9
215,364 - +0.2
215,382 + +0.6
215,408 + -0.1
215,416 - +0.5
215,454 + -0.1
215,454 + -1.1
215,473 - +1.8
215,509 - CCNA_00201 0.13 -0.3
215,509 - CCNA_00201 0.13 +1.0
215,541 + CCNA_00201 0.18 -0.3
215,568 + CCNA_00201 0.22 -0.6
215,568 + CCNA_00201 0.22 -0.2
215,568 + CCNA_00201 0.22 -1.3
215,568 + CCNA_00201 0.22 -0.7
215,568 + CCNA_00201 0.22 -0.8
215,568 + CCNA_00201 0.22 -0.6
215,568 + CCNA_00201 0.22 -0.5
215,568 + CCNA_00201 0.22 +1.1
215,568 + CCNA_00201 0.22 -1.1
215,568 + CCNA_00201 0.22 +0.4
215,576 - CCNA_00201 0.23 +0.0
215,576 - CCNA_00201 0.23 -0.0
215,576 - CCNA_00201 0.23 -1.4
215,576 - CCNA_00201 0.23 -1.2
215,576 - CCNA_00201 0.23 -0.8
215,576 - CCNA_00201 0.23 -0.6
215,581 - CCNA_00201 0.24 -0.1
215,595 - CCNA_00201 0.26 +1.7
215,642 - CCNA_00201 0.33 -0.7
215,677 - CCNA_00201 0.38 -0.3
215,704 + CCNA_00201 0.42 +0.2
215,756 + CCNA_00201 0.50 +0.5
215,759 - CCNA_00201 0.50 -0.1
215,764 - CCNA_00201 0.51 -0.2
215,791 + CCNA_00201 0.55 -0.8
215,796 + CCNA_00201 0.56 +2.0
215,816 - CCNA_00201 0.58 +0.4
215,816 - CCNA_00201 0.58 -1.0
215,821 - CCNA_00201 0.59 -0.4
215,916 - CCNA_00201 0.73 -0.7
215,922 + CCNA_00201 0.74 +0.1
215,922 + CCNA_00201 0.74 -0.2
215,930 - CCNA_00201 0.75 -1.0
215,979 + CCNA_00201 0.82 +0.1
216,021 - CCNA_00201 0.89 -0.5
216,035 - -0.7
216,043 - -0.6
216,054 + +0.2
216,062 - +0.5
216,087 + +0.2
216,087 + +0.0
216,092 + +0.2
216,094 + +0.2
216,143 - +0.8
216,168 + -0.1
216,187 + -0.2
216,195 - -0.1
216,197 - +0.9
216,207 + -0.3
216,210 - +0.2
216,234 + -1.1
216,253 + +0.5
216,255 + -0.3
216,281 + +0.1
216,301 - -0.1
216,306 - -0.2
216,334 - CCNA_00202 0.11 -0.7
216,339 - CCNA_00202 0.12 +0.4
216,376 - CCNA_00202 0.15 -0.3
216,380 + CCNA_00202 0.16 +0.0
216,383 - CCNA_00202 0.16 -0.5
216,388 - CCNA_00202 0.16 -0.0
216,395 + CCNA_00202 0.17 -0.4
216,446 + CCNA_00202 0.22 -0.2
216,461 + CCNA_00202 0.23 -0.0
216,502 - CCNA_00202 0.27 -1.6
216,529 + CCNA_00202 0.30 -0.0
216,532 + CCNA_00202 0.30 -0.9
216,540 - CCNA_00202 0.31 +0.4
216,557 - CCNA_00202 0.32 +0.2
216,559 + CCNA_00202 0.33 +0.3
216,582 + CCNA_00202 0.35 +1.9
216,590 - CCNA_00202 0.36 -1.2
216,595 - CCNA_00202 0.36 -0.3
216,607 + CCNA_00202 0.37 +0.2
216,622 + CCNA_00202 0.39 +0.7
216,669 + CCNA_00202 0.43 -0.3
216,672 - CCNA_00202 0.43 -0.0
216,674 + CCNA_00202 0.43 +0.1
216,682 - CCNA_00202 0.44 -0.3
216,682 - CCNA_00202 0.44 +0.4
216,738 + CCNA_00202 0.50 +0.2
216,740 + CCNA_00202 0.50 +0.3
216,785 + CCNA_00202 0.54 +0.0
216,785 + CCNA_00202 0.54 +0.4
216,785 + CCNA_00202 0.54 +0.1
216,785 + CCNA_00202 0.54 -0.7
216,793 - CCNA_00202 0.55 -0.2
216,793 - CCNA_00202 0.55 +0.5
216,793 - CCNA_00202 0.55 -1.1
216,793 - CCNA_00202 0.55 +0.1
216,804 + CCNA_00202 0.56 +0.2
216,805 + CCNA_00202 0.56 +0.3
216,806 - CCNA_00202 0.56 +0.4
216,840 + CCNA_00202 0.59 +0.8
216,848 - CCNA_00202 0.60 -0.9
216,860 + CCNA_00202 0.61 -0.3
216,860 + CCNA_00202 0.61 +0.4
216,866 + CCNA_00202 0.62 -0.1
216,929 + CCNA_00202 0.68 +0.3
216,956 - CCNA_00202 0.70 -0.5
216,970 - CCNA_00202 0.71 +0.6
216,990 + CCNA_00202 0.73 +0.6
217,054 + CCNA_00202 0.79 -0.4
217,072 + CCNA_00202 0.81 +0.4
217,082 - CCNA_00202 0.82 -0.4
217,084 + CCNA_00202 0.82 +1.4
217,091 - CCNA_00202 0.83 +0.4
217,121 + CCNA_00202 0.86 +0.7
217,131 + CCNA_00202 0.87 -0.6
217,155 + CCNA_00202 0.89 +0.1
217,163 - CCNA_00202 0.90 -0.5
217,170 + +0.5
217,176 - +0.8
217,188 - -0.0
217,222 - -1.6
217,327 + +0.6
217,333 - +0.3
217,342 + -0.1
217,346 + +0.4
217,385 + -0.2
217,390 + +0.5
217,403 - +0.5
217,420 - +1.4
217,420 - +0.1
217,435 + CCNA_00203 0.11 -1.9
217,437 - CCNA_00203 0.11 +0.8
217,480 - CCNA_00203 0.15 -2.8
217,480 - CCNA_00203 0.15 -1.9
217,480 - CCNA_00203 0.15 +0.1
217,566 + CCNA_00203 0.24 +0.7
217,571 + CCNA_00203 0.24 -0.0
217,630 + CCNA_00203 0.30 +0.0
217,632 + CCNA_00203 0.30 -0.1
217,633 - CCNA_00203 0.30 -2.7
217,638 - CCNA_00203 0.31 -0.4
217,655 + CCNA_00203 0.33 -0.1
217,655 + CCNA_00203 0.33 +0.5
217,656 - CCNA_00203 0.33 -0.3
217,656 - CCNA_00203 0.33 +0.3
217,660 + CCNA_00203 0.33 -0.8
217,668 + CCNA_00203 0.34 +0.5
217,681 + CCNA_00203 0.35 -1.3
217,689 - CCNA_00203 0.36 -0.0
217,707 + CCNA_00203 0.38 +2.3
217,707 + CCNA_00203 0.38 -0.5
217,709 - CCNA_00203 0.38 -1.2
217,737 - CCNA_00203 0.41 +0.1
217,787 - CCNA_00203 0.46 +0.1
217,798 + CCNA_00203 0.47 -0.5
217,798 + CCNA_00203 0.47 +0.1
217,826 + CCNA_00203 0.50 -0.1
217,826 + CCNA_00203 0.50 -1.8
217,826 + CCNA_00203 0.50 -1.4
217,826 + CCNA_00203 0.50 +1.2
217,826 + CCNA_00203 0.50 -1.3
217,827 - CCNA_00203 0.50 -0.5
217,827 - CCNA_00203 0.50 +1.9
217,834 - CCNA_00203 0.50 +0.8
217,852 - CCNA_00203 0.52 +1.2
217,863 - CCNA_00203 0.53 -0.0
217,864 + CCNA_00203 0.53 +0.6
217,886 + CCNA_00203 0.56 +0.3
217,888 - CCNA_00203 0.56 -0.1
217,905 + CCNA_00203 0.57 +0.5
217,910 + CCNA_00203 0.58 +0.3
217,927 + CCNA_00203 0.60 +1.0
217,927 + CCNA_00203 0.60 +0.1
217,937 + CCNA_00203 0.61 +0.3
217,937 + CCNA_00203 0.61 +0.6
217,937 + CCNA_00203 0.61 +0.3
217,942 + CCNA_00203 0.61 +1.0
217,945 - CCNA_00203 0.61 -0.2
217,945 - CCNA_00203 0.61 -0.9
217,961 + CCNA_00203 0.63 +0.3
217,973 + CCNA_00203 0.64 +0.5
218,080 + CCNA_00203 0.75 +0.6
218,080 + CCNA_00203 0.75 +0.3
218,085 + CCNA_00203 0.75 -0.2
218,085 + CCNA_00203 0.75 +0.1
218,085 + CCNA_00203 0.75 +0.5
218,088 + CCNA_00203 0.76 +0.1
218,088 - CCNA_00203 0.76 +0.0
218,093 - CCNA_00203 0.76 -2.2
218,099 - CCNA_00203 0.77 +1.4
218,115 - CCNA_00203 0.78 -0.2
218,120 - CCNA_00203 0.79 +0.2
218,138 + CCNA_00203 0.81 -0.4
218,141 - CCNA_00203 0.81 +1.0
218,173 + CCNA_00203 0.84 +0.3
218,181 - CCNA_00203 0.85 -0.7
218,183 + CCNA_00203 0.85 +0.1
218,218 - CCNA_00203 0.89 -0.4
218,259 + +0.1

Or see this region's nucleotide sequence