Strain Fitness in Caulobacter crescentus NA1000 Δfur around CCNA_00194

Experiment: PYE with CHIR-090 4 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00193 and CCNA_00194 are separated by 1 nucleotidesCCNA_00194 and CCNA_00195 are separated by 141 nucleotidesCCNA_00195 and CCNA_00196 overlap by 4 nucleotides CCNA_00193: CCNA_00193 - 3-isopropylmalate dehydrogenase, at 206,989 to 208,041 _00193 CCNA_00194: CCNA_00194 - Antibiotic biosynthesis monooxygenase family protein, at 208,043 to 208,345 _00194 CCNA_00195: CCNA_00195 - 3-isopropylmalate dehydratase small subunit, at 208,487 to 209,095 _00195 CCNA_00196: CCNA_00196 - 3-isopropylmalate dehydratase large subunit, at 209,092 to 210,531 _00196 Position (kb) 208 209Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 207.045 kb on + strandat 207.045 kb on + strandat 207.045 kb on + strandat 207.064 kb on + strandat 207.072 kb on - strandat 207.107 kb on + strand, within CCNA_00193at 207.107 kb on + strand, within CCNA_00193at 207.138 kb on - strand, within CCNA_00193at 207.138 kb on - strand, within CCNA_00193at 207.143 kb on - strand, within CCNA_00193at 207.143 kb on - strand, within CCNA_00193at 207.172 kb on + strand, within CCNA_00193at 207.172 kb on + strand, within CCNA_00193at 207.180 kb on - strand, within CCNA_00193at 207.193 kb on + strand, within CCNA_00193at 207.201 kb on - strand, within CCNA_00193at 207.233 kb on - strand, within CCNA_00193at 207.233 kb on - strand, within CCNA_00193at 207.254 kb on + strand, within CCNA_00193at 207.321 kb on + strand, within CCNA_00193at 207.321 kb on + strand, within CCNA_00193at 207.348 kb on + strand, within CCNA_00193at 207.374 kb on + strand, within CCNA_00193at 207.398 kb on - strand, within CCNA_00193at 207.401 kb on + strand, within CCNA_00193at 207.437 kb on + strand, within CCNA_00193at 207.440 kb on - strand, within CCNA_00193at 207.460 kb on + strand, within CCNA_00193at 207.462 kb on - strand, within CCNA_00193at 207.467 kb on - strand, within CCNA_00193at 207.473 kb on + strand, within CCNA_00193at 207.499 kb on - strand, within CCNA_00193at 207.531 kb on - strand, within CCNA_00193at 207.544 kb on - strand, within CCNA_00193at 207.544 kb on - strand, within CCNA_00193at 207.563 kb on + strand, within CCNA_00193at 207.563 kb on + strand, within CCNA_00193at 207.615 kb on - strand, within CCNA_00193at 207.672 kb on - strand, within CCNA_00193at 207.677 kb on + strand, within CCNA_00193at 207.677 kb on + strand, within CCNA_00193at 207.695 kb on - strand, within CCNA_00193at 207.695 kb on - strand, within CCNA_00193at 207.695 kb on - strand, within CCNA_00193at 207.707 kb on + strand, within CCNA_00193at 207.720 kb on - strand, within CCNA_00193at 207.720 kb on - strand, within CCNA_00193at 207.774 kb on + strand, within CCNA_00193at 207.782 kb on - strand, within CCNA_00193at 207.844 kb on + strand, within CCNA_00193at 207.844 kb on + strand, within CCNA_00193at 207.852 kb on - strand, within CCNA_00193at 207.852 kb on - strand, within CCNA_00193at 207.857 kb on - strand, within CCNA_00193at 207.862 kb on + strand, within CCNA_00193at 207.884 kb on + strand, within CCNA_00193at 207.889 kb on + strand, within CCNA_00193at 207.906 kb on + strand, within CCNA_00193at 207.944 kb on + strandat 207.959 kb on - strandat 207.985 kb on - strandat 207.987 kb on - strandat 208.026 kb on + strandat 208.048 kb on + strandat 208.058 kb on + strandat 208.090 kb on + strand, within CCNA_00194at 208.098 kb on - strand, within CCNA_00194at 208.098 kb on - strand, within CCNA_00194at 208.105 kb on - strand, within CCNA_00194at 208.188 kb on + strand, within CCNA_00194at 208.196 kb on - strand, within CCNA_00194at 208.226 kb on + strand, within CCNA_00194at 208.227 kb on + strand, within CCNA_00194at 208.231 kb on + strand, within CCNA_00194at 208.234 kb on - strand, within CCNA_00194at 208.234 kb on - strand, within CCNA_00194at 208.260 kb on + strand, within CCNA_00194at 208.260 kb on + strand, within CCNA_00194at 208.260 kb on + strand, within CCNA_00194at 208.260 kb on + strand, within CCNA_00194at 208.260 kb on + strand, within CCNA_00194at 208.268 kb on - strand, within CCNA_00194at 208.268 kb on - strand, within CCNA_00194at 208.268 kb on - strand, within CCNA_00194at 208.268 kb on - strand, within CCNA_00194at 208.348 kb on - strandat 208.348 kb on - strandat 208.397 kb on + strandat 208.404 kb on + strandat 208.412 kb on - strandat 208.420 kb on + strandat 208.422 kb on + strandat 208.439 kb on - strandat 208.505 kb on - strandat 208.534 kb on + strandat 208.534 kb on + strandat 208.573 kb on + strand, within CCNA_00195at 208.634 kb on + strand, within CCNA_00195at 208.661 kb on - strand, within CCNA_00195at 208.666 kb on + strand, within CCNA_00195at 208.684 kb on - strand, within CCNA_00195at 208.700 kb on - strand, within CCNA_00195at 208.704 kb on + strand, within CCNA_00195at 208.712 kb on - strand, within CCNA_00195at 208.714 kb on + strand, within CCNA_00195at 208.723 kb on + strand, within CCNA_00195at 208.731 kb on - strand, within CCNA_00195at 208.741 kb on - strand, within CCNA_00195at 208.765 kb on - strand, within CCNA_00195at 208.772 kb on + strand, within CCNA_00195at 208.818 kb on + strand, within CCNA_00195at 208.870 kb on - strand, within CCNA_00195at 208.888 kb on + strand, within CCNA_00195at 208.896 kb on + strand, within CCNA_00195at 208.898 kb on + strand, within CCNA_00195at 208.898 kb on + strand, within CCNA_00195at 208.898 kb on + strand, within CCNA_00195at 208.904 kb on - strand, within CCNA_00195at 208.906 kb on - strand, within CCNA_00195at 208.906 kb on - strand, within CCNA_00195at 208.906 kb on - strand, within CCNA_00195at 208.906 kb on - strand, within CCNA_00195at 208.906 kb on - strand, within CCNA_00195at 208.918 kb on + strand, within CCNA_00195at 208.979 kb on + strand, within CCNA_00195at 209.001 kb on + strand, within CCNA_00195at 209.001 kb on + strand, within CCNA_00195at 209.001 kb on + strand, within CCNA_00195at 209.001 kb on + strand, within CCNA_00195at 209.001 kb on + strand, within CCNA_00195at 209.001 kb on + strand, within CCNA_00195at 209.001 kb on + strand, within CCNA_00195at 209.001 kb on + strand, within CCNA_00195at 209.009 kb on - strand, within CCNA_00195at 209.009 kb on - strand, within CCNA_00195at 209.009 kb on - strand, within CCNA_00195at 209.014 kb on + strand, within CCNA_00195at 209.014 kb on + strand, within CCNA_00195at 209.022 kb on - strand, within CCNA_00195at 209.022 kb on - strand, within CCNA_00195at 209.022 kb on - strand, within CCNA_00195at 209.022 kb on - strand, within CCNA_00195at 209.022 kb on - strand, within CCNA_00195at 209.024 kb on + strand, within CCNA_00195at 209.024 kb on + strand, within CCNA_00195at 209.032 kb on - strand, within CCNA_00195at 209.032 kb on - strand, within CCNA_00195at 209.048 kb on - strandat 209.089 kb on - strandat 209.179 kb on - strandat 209.332 kb on + strand, within CCNA_00196

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL
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207,045 + +0.4
207,045 + +0.1
207,045 + -0.4
207,064 + -1.1
207,072 - -0.6
207,107 + CCNA_00193 0.11 -0.3
207,107 + CCNA_00193 0.11 +0.4
207,138 - CCNA_00193 0.14 +0.8
207,138 - CCNA_00193 0.14 -0.3
207,143 - CCNA_00193 0.15 +0.0
207,143 - CCNA_00193 0.15 -0.7
207,172 + CCNA_00193 0.17 +1.3
207,172 + CCNA_00193 0.17 -0.0
207,180 - CCNA_00193 0.18 +0.3
207,193 + CCNA_00193 0.19 -0.9
207,201 - CCNA_00193 0.20 +0.6
207,233 - CCNA_00193 0.23 +0.6
207,233 - CCNA_00193 0.23 -0.2
207,254 + CCNA_00193 0.25 +0.3
207,321 + CCNA_00193 0.32 -3.8
207,321 + CCNA_00193 0.32 -0.7
207,348 + CCNA_00193 0.34 -0.5
207,374 + CCNA_00193 0.37 +1.2
207,398 - CCNA_00193 0.39 +0.8
207,401 + CCNA_00193 0.39 -0.9
207,437 + CCNA_00193 0.43 -0.4
207,440 - CCNA_00193 0.43 -1.4
207,460 + CCNA_00193 0.45 +0.4
207,462 - CCNA_00193 0.45 +0.4
207,467 - CCNA_00193 0.45 +0.1
207,473 + CCNA_00193 0.46 +1.2
207,499 - CCNA_00193 0.48 -0.8
207,531 - CCNA_00193 0.51 -0.8
207,544 - CCNA_00193 0.53 -0.2
207,544 - CCNA_00193 0.53 -0.6
207,563 + CCNA_00193 0.55 -0.1
207,563 + CCNA_00193 0.55 +0.7
207,615 - CCNA_00193 0.59 +0.8
207,672 - CCNA_00193 0.65 -0.0
207,677 + CCNA_00193 0.65 +0.1
207,677 + CCNA_00193 0.65 +0.3
207,695 - CCNA_00193 0.67 -1.1
207,695 - CCNA_00193 0.67 -0.5
207,695 - CCNA_00193 0.67 +0.0
207,707 + CCNA_00193 0.68 -0.6
207,720 - CCNA_00193 0.69 +0.4
207,720 - CCNA_00193 0.69 +0.3
207,774 + CCNA_00193 0.75 -0.3
207,782 - CCNA_00193 0.75 +0.8
207,844 + CCNA_00193 0.81 -0.4
207,844 + CCNA_00193 0.81 -0.0
207,852 - CCNA_00193 0.82 +0.8
207,852 - CCNA_00193 0.82 +0.5
207,857 - CCNA_00193 0.82 +0.3
207,862 + CCNA_00193 0.83 -0.1
207,884 + CCNA_00193 0.85 -0.6
207,889 + CCNA_00193 0.85 +1.1
207,906 + CCNA_00193 0.87 -0.2
207,944 + +1.0
207,959 - -1.5
207,985 - +0.1
207,987 - -0.6
208,026 + +0.4
208,048 + -0.3
208,058 + -0.4
208,090 + CCNA_00194 0.16 +0.1
208,098 - CCNA_00194 0.18 +0.9
208,098 - CCNA_00194 0.18 +0.4
208,105 - CCNA_00194 0.20 -0.4
208,188 + CCNA_00194 0.48 +0.4
208,196 - CCNA_00194 0.50 -0.2
208,226 + CCNA_00194 0.60 -0.3
208,227 + CCNA_00194 0.61 +0.2
208,231 + CCNA_00194 0.62 -0.0
208,234 - CCNA_00194 0.63 +0.1
208,234 - CCNA_00194 0.63 +0.9
208,260 + CCNA_00194 0.72 +0.3
208,260 + CCNA_00194 0.72 +0.6
208,260 + CCNA_00194 0.72 +0.1
208,260 + CCNA_00194 0.72 -0.2
208,260 + CCNA_00194 0.72 -0.0
208,268 - CCNA_00194 0.74 -2.7
208,268 - CCNA_00194 0.74 +0.2
208,268 - CCNA_00194 0.74 -0.2
208,268 - CCNA_00194 0.74 -0.7
208,348 - +0.6
208,348 - +0.5
208,397 + +0.9
208,404 + -0.1
208,412 - -0.4
208,420 + -0.7
208,422 + +0.1
208,439 - +0.5
208,505 - -0.3
208,534 + +1.7
208,534 + +0.6
208,573 + CCNA_00195 0.14 -0.8
208,634 + CCNA_00195 0.24 +0.5
208,661 - CCNA_00195 0.29 +0.0
208,666 + CCNA_00195 0.29 +0.0
208,684 - CCNA_00195 0.32 +1.2
208,700 - CCNA_00195 0.35 +1.8
208,704 + CCNA_00195 0.36 -0.3
208,712 - CCNA_00195 0.37 +0.8
208,714 + CCNA_00195 0.37 -0.0
208,723 + CCNA_00195 0.39 -0.5
208,731 - CCNA_00195 0.40 +0.6
208,741 - CCNA_00195 0.42 +0.3
208,765 - CCNA_00195 0.46 +0.0
208,772 + CCNA_00195 0.47 +0.7
208,818 + CCNA_00195 0.54 +0.6
208,870 - CCNA_00195 0.63 +0.5
208,888 + CCNA_00195 0.66 -0.8
208,896 + CCNA_00195 0.67 -0.3
208,898 + CCNA_00195 0.67 -0.5
208,898 + CCNA_00195 0.67 +0.1
208,898 + CCNA_00195 0.67 +0.5
208,904 - CCNA_00195 0.68 -0.7
208,906 - CCNA_00195 0.69 +0.0
208,906 - CCNA_00195 0.69 +1.1
208,906 - CCNA_00195 0.69 -0.6
208,906 - CCNA_00195 0.69 +0.1
208,906 - CCNA_00195 0.69 -0.1
208,918 + CCNA_00195 0.71 -0.9
208,979 + CCNA_00195 0.81 +1.7
209,001 + CCNA_00195 0.84 -1.8
209,001 + CCNA_00195 0.84 -0.7
209,001 + CCNA_00195 0.84 +0.1
209,001 + CCNA_00195 0.84 +0.1
209,001 + CCNA_00195 0.84 -0.0
209,001 + CCNA_00195 0.84 -0.9
209,001 + CCNA_00195 0.84 -0.3
209,001 + CCNA_00195 0.84 -1.8
209,009 - CCNA_00195 0.86 +0.4
209,009 - CCNA_00195 0.86 -0.2
209,009 - CCNA_00195 0.86 -0.1
209,014 + CCNA_00195 0.87 +1.6
209,014 + CCNA_00195 0.87 -0.2
209,022 - CCNA_00195 0.88 -0.4
209,022 - CCNA_00195 0.88 -0.2
209,022 - CCNA_00195 0.88 -0.6
209,022 - CCNA_00195 0.88 -0.0
209,022 - CCNA_00195 0.88 -1.3
209,024 + CCNA_00195 0.88 +1.4
209,024 + CCNA_00195 0.88 -0.6
209,032 - CCNA_00195 0.89 -0.8
209,032 - CCNA_00195 0.89 +0.5
209,048 - +0.6
209,089 - +1.1
209,179 - -0.2
209,332 + CCNA_00196 0.17 +0.6

Or see this region's nucleotide sequence