Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00092 and CCNA_00093 are separated by 98 nucleotides CCNA_00093 and CCNA_00094 are separated by 72 nucleotides CCNA_00094 and CCNA_00095 are separated by 103 nucleotides
CCNA_00092: CCNA_00092 - short chain dehydrogenase, at 104,039 to 104,929
_00092
CCNA_00093: CCNA_00093 - hypothetical protein, at 105,028 to 105,237
_00093
CCNA_00094: CCNA_00094 - WecG/TagA-family glycosyltransferase HfsJ, at 105,310 to 106,230
_00094
CCNA_00095: CCNA_00095 - quinone oxidoreductase, at 106,334 to 107,332
_00095
Position (kb) 105
106
107 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 104.338 kb on + strand, within CCNA_00092 at 104.356 kb on + strand, within CCNA_00092 at 104.382 kb on - strand, within CCNA_00092 at 104.382 kb on - strand, within CCNA_00092 at 104.462 kb on - strand, within CCNA_00092 at 104.466 kb on + strand, within CCNA_00092 at 104.471 kb on + strand, within CCNA_00092 at 104.479 kb on - strand, within CCNA_00092 at 104.479 kb on - strand, within CCNA_00092 at 104.486 kb on - strand, within CCNA_00092 at 104.487 kb on - strand, within CCNA_00092 at 104.516 kb on + strand, within CCNA_00092 at 104.522 kb on - strand, within CCNA_00092 at 104.550 kb on + strand, within CCNA_00092 at 104.557 kb on - strand, within CCNA_00092 at 104.559 kb on - strand, within CCNA_00092 at 104.570 kb on + strand, within CCNA_00092 at 104.586 kb on + strand, within CCNA_00092 at 104.604 kb on - strand, within CCNA_00092 at 104.629 kb on + strand, within CCNA_00092 at 104.629 kb on + strand, within CCNA_00092 at 104.632 kb on - strand, within CCNA_00092 at 104.637 kb on - strand, within CCNA_00092 at 104.637 kb on - strand, within CCNA_00092 at 104.637 kb on - strand, within CCNA_00092 at 104.637 kb on - strand, within CCNA_00092 at 104.676 kb on + strand, within CCNA_00092 at 104.681 kb on + strand, within CCNA_00092 at 104.689 kb on - strand, within CCNA_00092 at 104.697 kb on - strand, within CCNA_00092 at 104.708 kb on - strand, within CCNA_00092 at 104.711 kb on + strand, within CCNA_00092 at 104.712 kb on + strand, within CCNA_00092 at 104.712 kb on + strand, within CCNA_00092 at 104.719 kb on - strand, within CCNA_00092 at 104.720 kb on - strand, within CCNA_00092 at 104.745 kb on + strand, within CCNA_00092 at 104.770 kb on + strand, within CCNA_00092 at 104.770 kb on + strand, within CCNA_00092 at 104.778 kb on - strand, within CCNA_00092 at 104.783 kb on + strand, within CCNA_00092 at 104.785 kb on + strand, within CCNA_00092 at 104.785 kb on + strand, within CCNA_00092 at 104.793 kb on - strand, within CCNA_00092 at 104.793 kb on - strand, within CCNA_00092 at 104.854 kb on + strand at 104.871 kb on - strand at 104.871 kb on - strand at 104.875 kb on + strand at 104.885 kb on + strand at 104.893 kb on - strand at 104.920 kb on - strand at 104.969 kb on - strand at 104.998 kb on + strand at 105.006 kb on - strand at 105.080 kb on + strand, within CCNA_00093 at 105.104 kb on - strand, within CCNA_00093 at 105.113 kb on + strand, within CCNA_00093 at 105.131 kb on + strand, within CCNA_00093 at 105.131 kb on + strand, within CCNA_00093 at 105.131 kb on + strand, within CCNA_00093 at 105.131 kb on + strand, within CCNA_00093 at 105.131 kb on + strand, within CCNA_00093 at 105.131 kb on + strand, within CCNA_00093 at 105.136 kb on + strand, within CCNA_00093 at 105.136 kb on + strand, within CCNA_00093 at 105.136 kb on + strand, within CCNA_00093 at 105.139 kb on - strand, within CCNA_00093 at 105.139 kb on - strand, within CCNA_00093 at 105.139 kb on - strand, within CCNA_00093 at 105.149 kb on - strand, within CCNA_00093 at 105.149 kb on - strand, within CCNA_00093 at 105.156 kb on + strand, within CCNA_00093 at 105.165 kb on - strand, within CCNA_00093 at 105.165 kb on - strand, within CCNA_00093 at 105.167 kb on + strand, within CCNA_00093 at 105.203 kb on + strand, within CCNA_00093 at 105.204 kb on + strand, within CCNA_00093 at 105.337 kb on + strand at 105.340 kb on - strand at 105.345 kb on - strand at 105.389 kb on + strand at 105.397 kb on - strand at 105.397 kb on - strand at 105.415 kb on + strand, within CCNA_00094 at 105.447 kb on + strand, within CCNA_00094 at 105.487 kb on - strand, within CCNA_00094 at 105.493 kb on + strand, within CCNA_00094 at 105.496 kb on + strand, within CCNA_00094 at 105.502 kb on + strand, within CCNA_00094 at 105.504 kb on + strand, within CCNA_00094 at 105.536 kb on + strand, within CCNA_00094 at 105.538 kb on + strand, within CCNA_00094 at 105.586 kb on - strand, within CCNA_00094 at 105.602 kb on + strand, within CCNA_00094 at 105.602 kb on - strand, within CCNA_00094 at 105.604 kb on - strand, within CCNA_00094 at 105.620 kb on + strand, within CCNA_00094 at 105.628 kb on - strand, within CCNA_00094 at 105.703 kb on + strand, within CCNA_00094 at 105.703 kb on + strand, within CCNA_00094 at 105.711 kb on - strand, within CCNA_00094 at 105.723 kb on - strand, within CCNA_00094 at 105.754 kb on - strand, within CCNA_00094 at 105.769 kb on + strand, within CCNA_00094 at 105.769 kb on + strand, within CCNA_00094 at 105.769 kb on + strand, within CCNA_00094 at 105.769 kb on + strand, within CCNA_00094 at 105.850 kb on + strand, within CCNA_00094 at 105.856 kb on + strand, within CCNA_00094 at 105.858 kb on - strand, within CCNA_00094 at 105.858 kb on - strand, within CCNA_00094 at 105.859 kb on + strand, within CCNA_00094 at 105.866 kb on - strand, within CCNA_00094 at 105.877 kb on + strand, within CCNA_00094 at 105.877 kb on + strand, within CCNA_00094 at 105.880 kb on - strand, within CCNA_00094 at 105.885 kb on - strand, within CCNA_00094 at 105.956 kb on + strand, within CCNA_00094 at 105.966 kb on + strand, within CCNA_00094 at 105.977 kb on + strand, within CCNA_00094 at 105.982 kb on + strand, within CCNA_00094 at 105.982 kb on - strand, within CCNA_00094 at 106.014 kb on + strand, within CCNA_00094 at 106.014 kb on + strand, within CCNA_00094 at 106.014 kb on + strand, within CCNA_00094 at 106.014 kb on + strand, within CCNA_00094 at 106.014 kb on + strand, within CCNA_00094 at 106.014 kb on + strand, within CCNA_00094 at 106.022 kb on - strand, within CCNA_00094 at 106.022 kb on - strand, within CCNA_00094 at 106.022 kb on - strand, within CCNA_00094 at 106.022 kb on - strand, within CCNA_00094 at 106.022 kb on - strand, within CCNA_00094 at 106.022 kb on - strand, within CCNA_00094 at 106.062 kb on + strand, within CCNA_00094 at 106.118 kb on + strand, within CCNA_00094 at 106.136 kb on - strand, within CCNA_00094 at 106.136 kb on - strand, within CCNA_00094 at 106.143 kb on + strand at 106.151 kb on - strand at 106.151 kb on - strand at 106.151 kb on - strand at 106.169 kb on - strand at 106.193 kb on + strand at 106.197 kb on + strand at 106.205 kb on - strand at 106.240 kb on + strand at 106.242 kb on + strand at 106.248 kb on - strand at 106.330 kb on + strand at 106.341 kb on + strand at 106.352 kb on - strand at 106.353 kb on + strand at 106.399 kb on - strand at 106.406 kb on + strand at 106.414 kb on - strand at 106.452 kb on + strand, within CCNA_00095 at 106.460 kb on - strand, within CCNA_00095 at 106.478 kb on + strand, within CCNA_00095 at 106.478 kb on + strand, within CCNA_00095 at 106.485 kb on + strand, within CCNA_00095 at 106.492 kb on + strand, within CCNA_00095 at 106.518 kb on + strand, within CCNA_00095 at 106.518 kb on + strand, within CCNA_00095 at 106.518 kb on + strand, within CCNA_00095 at 106.518 kb on + strand, within CCNA_00095 at 106.520 kb on + strand, within CCNA_00095 at 106.526 kb on - strand, within CCNA_00095 at 106.526 kb on - strand, within CCNA_00095 at 106.526 kb on - strand, within CCNA_00095 at 106.526 kb on - strand, within CCNA_00095 at 106.526 kb on - strand, within CCNA_00095 at 106.526 kb on - strand, within CCNA_00095 at 106.526 kb on - strand, within CCNA_00095 at 106.547 kb on + strand, within CCNA_00095 at 106.555 kb on + strand, within CCNA_00095 at 106.625 kb on - strand, within CCNA_00095 at 106.650 kb on - strand, within CCNA_00095 at 106.650 kb on - strand, within CCNA_00095 at 106.678 kb on + strand, within CCNA_00095 at 106.678 kb on + strand, within CCNA_00095 at 106.688 kb on - strand, within CCNA_00095 at 106.704 kb on + strand, within CCNA_00095 at 106.712 kb on - strand, within CCNA_00095 at 106.712 kb on - strand, within CCNA_00095 at 106.767 kb on - strand, within CCNA_00095 at 106.767 kb on - strand, within CCNA_00095 at 106.842 kb on + strand, within CCNA_00095 at 106.853 kb on + strand, within CCNA_00095 at 106.853 kb on + strand, within CCNA_00095 at 106.856 kb on - strand, within CCNA_00095 at 106.856 kb on - strand, within CCNA_00095 at 106.856 kb on - strand, within CCNA_00095 at 106.856 kb on - strand, within CCNA_00095 at 106.856 kb on - strand, within CCNA_00095 at 106.932 kb on + strand, within CCNA_00095 at 106.949 kb on + strand, within CCNA_00095 at 106.949 kb on + strand, within CCNA_00095 at 106.950 kb on - strand, within CCNA_00095 at 106.957 kb on - strand, within CCNA_00095 at 106.964 kb on + strand, within CCNA_00095 at 106.964 kb on + strand, within CCNA_00095 at 106.969 kb on + strand, within CCNA_00095 at 106.972 kb on - strand, within CCNA_00095 at 107.003 kb on + strand, within CCNA_00095 at 107.037 kb on - strand, within CCNA_00095 at 107.075 kb on - strand, within CCNA_00095 at 107.091 kb on + strand, within CCNA_00095 at 107.092 kb on + strand, within CCNA_00095 at 107.102 kb on + strand, within CCNA_00095 at 107.102 kb on + strand, within CCNA_00095 at 107.107 kb on + strand, within CCNA_00095 at 107.119 kb on + strand, within CCNA_00095 at 107.126 kb on + strand, within CCNA_00095 at 107.138 kb on - strand, within CCNA_00095 at 107.141 kb on + strand, within CCNA_00095 at 107.212 kb on + strand, within CCNA_00095 at 107.220 kb on + strand, within CCNA_00095
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 104,338 + CCNA_00092 0.34 -0.4 104,356 + CCNA_00092 0.36 +0.3 104,382 - CCNA_00092 0.38 +0.4 104,382 - CCNA_00092 0.38 -0.9 104,462 - CCNA_00092 0.47 -1.5 104,466 + CCNA_00092 0.48 -0.2 104,471 + CCNA_00092 0.48 +0.2 104,479 - CCNA_00092 0.49 -0.0 104,479 - CCNA_00092 0.49 +0.4 104,486 - CCNA_00092 0.50 -0.2 104,487 - CCNA_00092 0.50 -0.8 104,516 + CCNA_00092 0.54 -0.2 104,522 - CCNA_00092 0.54 -0.8 104,550 + CCNA_00092 0.57 -0.3 104,557 - CCNA_00092 0.58 +0.8 104,559 - CCNA_00092 0.58 +1.5 104,570 + CCNA_00092 0.60 -0.3 104,586 + CCNA_00092 0.61 -0.8 104,604 - CCNA_00092 0.63 -0.1 104,629 + CCNA_00092 0.66 +1.2 104,629 + CCNA_00092 0.66 -1.2 104,632 - CCNA_00092 0.67 -1.7 104,637 - CCNA_00092 0.67 +0.2 104,637 - CCNA_00092 0.67 +0.5 104,637 - CCNA_00092 0.67 -0.1 104,637 - CCNA_00092 0.67 -0.1 104,676 + CCNA_00092 0.71 -0.8 104,681 + CCNA_00092 0.72 -0.4 104,689 - CCNA_00092 0.73 +0.2 104,697 - CCNA_00092 0.74 -0.5 104,708 - CCNA_00092 0.75 -0.2 104,711 + CCNA_00092 0.75 -0.2 104,712 + CCNA_00092 0.76 +0.0 104,712 + CCNA_00092 0.76 -1.2 104,719 - CCNA_00092 0.76 +0.8 104,720 - CCNA_00092 0.76 -0.2 104,745 + CCNA_00092 0.79 +0.0 104,770 + CCNA_00092 0.82 -1.9 104,770 + CCNA_00092 0.82 -0.3 104,778 - CCNA_00092 0.83 -0.5 104,783 + CCNA_00092 0.84 +0.2 104,785 + CCNA_00092 0.84 +1.1 104,785 + CCNA_00092 0.84 -0.6 104,793 - CCNA_00092 0.85 -0.1 104,793 - CCNA_00092 0.85 -0.1 104,854 + -2.0 104,871 - +0.0 104,871 - +0.2 104,875 + -0.7 104,885 + +0.6 104,893 - +0.0 104,920 - -0.1 104,969 - -0.2 104,998 + +1.5 105,006 - -0.5 105,080 + CCNA_00093 0.25 +0.5 105,104 - CCNA_00093 0.36 +0.1 105,113 + CCNA_00093 0.40 -1.9 105,131 + CCNA_00093 0.49 -2.6 105,131 + CCNA_00093 0.49 -0.3 105,131 + CCNA_00093 0.49 -2.4 105,131 + CCNA_00093 0.49 +1.0 105,131 + CCNA_00093 0.49 +0.1 105,131 + CCNA_00093 0.49 -0.2 105,136 + CCNA_00093 0.51 -1.1 105,136 + CCNA_00093 0.51 +1.2 105,136 + CCNA_00093 0.51 -3.0 105,139 - CCNA_00093 0.53 +1.0 105,139 - CCNA_00093 0.53 +0.3 105,139 - CCNA_00093 0.53 +0.0 105,149 - CCNA_00093 0.58 +0.1 105,149 - CCNA_00093 0.58 -0.3 105,156 + CCNA_00093 0.61 -0.7 105,165 - CCNA_00093 0.65 +0.1 105,165 - CCNA_00093 0.65 -0.5 105,167 + CCNA_00093 0.66 -1.4 105,203 + CCNA_00093 0.83 -1.1 105,204 + CCNA_00093 0.84 +1.7 105,337 + +0.3 105,340 - +0.1 105,345 - +0.2 105,389 + -1.0 105,397 - -0.0 105,397 - -0.5 105,415 + CCNA_00094 0.11 -2.0 105,447 + CCNA_00094 0.15 -0.6 105,487 - CCNA_00094 0.19 -0.4 105,493 + CCNA_00094 0.20 +1.1 105,496 + CCNA_00094 0.20 -2.5 105,502 + CCNA_00094 0.21 -3.1 105,504 + CCNA_00094 0.21 -0.2 105,536 + CCNA_00094 0.25 +0.1 105,538 + CCNA_00094 0.25 -1.4 105,586 - CCNA_00094 0.30 +0.9 105,602 + CCNA_00094 0.32 +0.5 105,602 - CCNA_00094 0.32 +0.1 105,604 - CCNA_00094 0.32 +0.2 105,620 + CCNA_00094 0.34 +0.2 105,628 - CCNA_00094 0.35 +0.4 105,703 + CCNA_00094 0.43 -0.3 105,703 + CCNA_00094 0.43 -1.4 105,711 - CCNA_00094 0.44 -0.1 105,723 - CCNA_00094 0.45 +0.3 105,754 - CCNA_00094 0.48 +0.4 105,769 + CCNA_00094 0.50 -0.5 105,769 + CCNA_00094 0.50 -1.0 105,769 + CCNA_00094 0.50 +0.6 105,769 + CCNA_00094 0.50 -3.5 105,850 + CCNA_00094 0.59 -1.1 105,856 + CCNA_00094 0.59 -0.7 105,858 - CCNA_00094 0.60 -0.3 105,858 - CCNA_00094 0.60 -0.9 105,859 + CCNA_00094 0.60 -0.5 105,866 - CCNA_00094 0.60 -0.2 105,877 + CCNA_00094 0.62 +0.3 105,877 + CCNA_00094 0.62 -1.2 105,880 - CCNA_00094 0.62 +1.4 105,885 - CCNA_00094 0.62 -0.6 105,956 + CCNA_00094 0.70 -0.3 105,966 + CCNA_00094 0.71 +0.4 105,977 + CCNA_00094 0.72 -1.3 105,982 + CCNA_00094 0.73 +0.3 105,982 - CCNA_00094 0.73 -0.9 106,014 + CCNA_00094 0.76 -0.3 106,014 + CCNA_00094 0.76 +0.3 106,014 + CCNA_00094 0.76 +2.4 106,014 + CCNA_00094 0.76 -0.5 106,014 + CCNA_00094 0.76 -0.9 106,014 + CCNA_00094 0.76 +0.3 106,022 - CCNA_00094 0.77 -0.1 106,022 - CCNA_00094 0.77 +0.2 106,022 - CCNA_00094 0.77 +0.0 106,022 - CCNA_00094 0.77 -0.1 106,022 - CCNA_00094 0.77 +1.4 106,022 - CCNA_00094 0.77 -0.9 106,062 + CCNA_00094 0.82 -0.1 106,118 + CCNA_00094 0.88 -0.8 106,136 - CCNA_00094 0.90 +0.1 106,136 - CCNA_00094 0.90 +0.3 106,143 + +0.3 106,151 - -0.1 106,151 - -0.4 106,151 - -0.1 106,169 - -0.5 106,193 + +0.6 106,197 + +0.3 106,205 - -1.1 106,240 + -0.4 106,242 + -0.2 106,248 - -0.1 106,330 + +0.2 106,341 + -0.6 106,352 - +0.0 106,353 + -0.3 106,399 - +0.1 106,406 + -0.9 106,414 - +0.6 106,452 + CCNA_00095 0.12 -0.1 106,460 - CCNA_00095 0.13 +0.6 106,478 + CCNA_00095 0.14 -1.8 106,478 + CCNA_00095 0.14 -2.3 106,485 + CCNA_00095 0.15 +1.8 106,492 + CCNA_00095 0.16 +1.4 106,518 + CCNA_00095 0.18 -0.7 106,518 + CCNA_00095 0.18 -1.3 106,518 + CCNA_00095 0.18 -0.4 106,518 + CCNA_00095 0.18 -0.9 106,520 + CCNA_00095 0.19 +0.7 106,526 - CCNA_00095 0.19 +0.4 106,526 - CCNA_00095 0.19 -0.6 106,526 - CCNA_00095 0.19 +0.2 106,526 - CCNA_00095 0.19 -0.1 106,526 - CCNA_00095 0.19 +0.2 106,526 - CCNA_00095 0.19 -0.3 106,526 - CCNA_00095 0.19 +0.3 106,547 + CCNA_00095 0.21 +0.1 106,555 + CCNA_00095 0.22 +0.3 106,625 - CCNA_00095 0.29 -0.2 106,650 - CCNA_00095 0.32 +0.4 106,650 - CCNA_00095 0.32 +0.1 106,678 + CCNA_00095 0.34 +0.4 106,678 + CCNA_00095 0.34 +0.7 106,688 - CCNA_00095 0.35 +0.4 106,704 + CCNA_00095 0.37 -0.9 106,712 - CCNA_00095 0.38 +0.3 106,712 - CCNA_00095 0.38 +0.0 106,767 - CCNA_00095 0.43 -1.2 106,767 - CCNA_00095 0.43 +0.0 106,842 + CCNA_00095 0.51 +0.2 106,853 + CCNA_00095 0.52 -0.4 106,853 + CCNA_00095 0.52 -0.9 106,856 - CCNA_00095 0.52 +0.6 106,856 - CCNA_00095 0.52 +1.1 106,856 - CCNA_00095 0.52 +0.4 106,856 - CCNA_00095 0.52 -0.9 106,856 - CCNA_00095 0.52 +0.3 106,932 + CCNA_00095 0.60 -1.0 106,949 + CCNA_00095 0.62 +0.0 106,949 + CCNA_00095 0.62 +0.2 106,950 - CCNA_00095 0.62 -0.2 106,957 - CCNA_00095 0.62 -0.1 106,964 + CCNA_00095 0.63 +0.1 106,964 + CCNA_00095 0.63 -0.3 106,969 + CCNA_00095 0.64 -0.8 106,972 - CCNA_00095 0.64 -0.7 107,003 + CCNA_00095 0.67 -1.6 107,037 - CCNA_00095 0.70 -0.3 107,075 - CCNA_00095 0.74 -1.0 107,091 + CCNA_00095 0.76 -0.9 107,092 + CCNA_00095 0.76 -0.0 107,102 + CCNA_00095 0.77 -1.4 107,102 + CCNA_00095 0.77 +1.0 107,107 + CCNA_00095 0.77 -0.8 107,119 + CCNA_00095 0.79 -0.5 107,126 + CCNA_00095 0.79 -2.0 107,138 - CCNA_00095 0.80 -0.8 107,141 + CCNA_00095 0.81 +0.3 107,212 + CCNA_00095 0.88 -0.1 107,220 + CCNA_00095 0.89 -0.1
Or see this region's nucleotide sequence