Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4252 and EX28DRAFT_4253 overlap by 1 nucleotides EX28DRAFT_4253 and EX28DRAFT_4254 are separated by 18 nucleotides EX28DRAFT_4254 and EX28DRAFT_4255 are separated by 65 nucleotides EX28DRAFT_4255 and EX28DRAFT_4256 are separated by 0 nucleotides EX28DRAFT_4256 and EX28DRAFT_4257 are separated by 81 nucleotides
EX28DRAFT_4252: EX28DRAFT_4252 - Protein of unknown function (DUF2589), at 59,258 to 59,860
_4252
EX28DRAFT_4253: EX28DRAFT_4253 - Phage-related lysozyme (muraminidase), at 59,860 to 60,309
_4253
EX28DRAFT_4254: EX28DRAFT_4254 - ribonuclease, at 60,328 to 60,810
_4254
EX28DRAFT_4255: EX28DRAFT_4255 - hypothetical protein, at 60,876 to 61,283
_4255
EX28DRAFT_4256: EX28DRAFT_4256 - hypothetical protein, at 61,284 to 61,574
_4256
EX28DRAFT_4257: EX28DRAFT_4257 - Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain, at 61,656 to 62,261
_4257
Position (kb)
60
61 Strain fitness (log2 ratio)
-1
0
1 at 59.408 kb on - strand, within EX28DRAFT_4252 at 59.408 kb on - strand, within EX28DRAFT_4252 at 59.426 kb on + strand, within EX28DRAFT_4252 at 59.465 kb on + strand, within EX28DRAFT_4252 at 59.465 kb on + strand, within EX28DRAFT_4252 at 59.467 kb on + strand, within EX28DRAFT_4252 at 59.467 kb on + strand, within EX28DRAFT_4252 at 59.467 kb on + strand, within EX28DRAFT_4252 at 59.467 kb on + strand, within EX28DRAFT_4252 at 59.468 kb on - strand, within EX28DRAFT_4252 at 59.814 kb on - strand at 59.880 kb on + strand at 59.881 kb on - strand at 60.238 kb on - strand, within EX28DRAFT_4253 at 60.296 kb on + strand at 60.304 kb on - strand at 60.307 kb on + strand at 60.426 kb on - strand, within EX28DRAFT_4254 at 60.426 kb on - strand, within EX28DRAFT_4254 at 60.460 kb on - strand, within EX28DRAFT_4254 at 60.508 kb on - strand, within EX28DRAFT_4254 at 60.539 kb on - strand, within EX28DRAFT_4254 at 60.540 kb on + strand, within EX28DRAFT_4254 at 60.541 kb on - strand, within EX28DRAFT_4254 at 60.643 kb on + strand, within EX28DRAFT_4254 at 60.733 kb on + strand, within EX28DRAFT_4254 at 60.775 kb on + strand at 60.776 kb on - strand at 60.776 kb on - strand at 60.776 kb on - strand at 60.776 kb on - strand at 60.783 kb on + strand at 60.784 kb on - strand at 60.808 kb on + strand at 60.809 kb on - strand at 60.809 kb on - strand at 60.830 kb on + strand at 60.830 kb on + strand at 60.875 kb on + strand at 60.875 kb on + strand at 60.876 kb on - strand at 60.876 kb on - strand at 60.889 kb on - strand at 60.998 kb on + strand, within EX28DRAFT_4255 at 60.999 kb on - strand, within EX28DRAFT_4255 at 60.999 kb on - strand, within EX28DRAFT_4255 at 61.010 kb on + strand, within EX28DRAFT_4255 at 61.079 kb on - strand, within EX28DRAFT_4255 at 61.119 kb on - strand, within EX28DRAFT_4255 at 61.136 kb on + strand, within EX28DRAFT_4255 at 61.137 kb on - strand, within EX28DRAFT_4255 at 61.295 kb on - strand at 61.369 kb on - strand, within EX28DRAFT_4256 at 61.662 kb on - strand at 61.669 kb on + strand at 61.740 kb on + strand, within EX28DRAFT_4257 at 61.740 kb on + strand, within EX28DRAFT_4257 at 61.741 kb on - strand, within EX28DRAFT_4257
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 59,408 - EX28DRAFT_4252 0.25 -0.1 59,408 - EX28DRAFT_4252 0.25 -0.2 59,426 + EX28DRAFT_4252 0.28 -0.2 59,465 + EX28DRAFT_4252 0.34 -0.8 59,465 + EX28DRAFT_4252 0.34 +0.1 59,467 + EX28DRAFT_4252 0.35 +0.0 59,467 + EX28DRAFT_4252 0.35 +0.4 59,467 + EX28DRAFT_4252 0.35 -0.3 59,467 + EX28DRAFT_4252 0.35 -0.4 59,468 - EX28DRAFT_4252 0.35 -0.8 59,814 - -1.2 59,880 + +0.2 59,881 - -0.2 60,238 - EX28DRAFT_4253 0.84 +0.6 60,296 + -0.2 60,304 - +0.2 60,307 + -0.5 60,426 - EX28DRAFT_4254 0.20 +0.6 60,426 - EX28DRAFT_4254 0.20 -0.2 60,460 - EX28DRAFT_4254 0.27 +0.6 60,508 - EX28DRAFT_4254 0.37 -0.0 60,539 - EX28DRAFT_4254 0.44 -0.4 60,540 + EX28DRAFT_4254 0.44 -1.0 60,541 - EX28DRAFT_4254 0.44 +0.9 60,643 + EX28DRAFT_4254 0.65 +0.4 60,733 + EX28DRAFT_4254 0.84 +0.6 60,775 + +0.8 60,776 - +0.5 60,776 - +0.2 60,776 - +0.3 60,776 - -0.7 60,783 + +1.0 60,784 - +0.1 60,808 + +0.1 60,809 - -0.8 60,809 - +0.2 60,830 + -0.4 60,830 + -0.4 60,875 + -0.2 60,875 + -0.1 60,876 - -1.3 60,876 - +0.1 60,889 - -0.3 60,998 + EX28DRAFT_4255 0.30 -0.5 60,999 - EX28DRAFT_4255 0.30 -0.3 60,999 - EX28DRAFT_4255 0.30 -0.1 61,010 + EX28DRAFT_4255 0.33 +0.2 61,079 - EX28DRAFT_4255 0.50 +0.2 61,119 - EX28DRAFT_4255 0.60 -0.1 61,136 + EX28DRAFT_4255 0.64 -0.3 61,137 - EX28DRAFT_4255 0.64 +0.4 61,295 - -0.4 61,369 - EX28DRAFT_4256 0.29 -0.2 61,662 - -0.7 61,669 + -0.2 61,740 + EX28DRAFT_4257 0.14 -0.6 61,740 + EX28DRAFT_4257 0.14 -0.7 61,741 - EX28DRAFT_4257 0.14 +1.7
Or see this region's nucleotide sequence