Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4056 and EX28DRAFT_4057 are separated by 119 nucleotides EX28DRAFT_4057 and EX28DRAFT_4058 are separated by 267 nucleotides EX28DRAFT_4058 and EX28DRAFT_4059 are separated by 148 nucleotides
EX28DRAFT_4056: EX28DRAFT_4056 - Transcriptional regulator, at 29,751 to 30,650
_4056
EX28DRAFT_4057: EX28DRAFT_4057 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 30,770 to 31,528
_4057
EX28DRAFT_4058: EX28DRAFT_4058 - Acetyltransferases, including N-acetylases of ribosomal proteins, at 31,796 to 32,335
_4058
EX28DRAFT_4059: EX28DRAFT_4059 - hypothetical protein, at 32,484 to 34,073
_4059
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-2
-1
0
1 at 29.781 kb on + strand at 29.936 kb on + strand, within EX28DRAFT_4056 at 30.035 kb on - strand, within EX28DRAFT_4056 at 30.139 kb on + strand, within EX28DRAFT_4056 at 30.139 kb on + strand, within EX28DRAFT_4056 at 30.458 kb on - strand, within EX28DRAFT_4056 at 30.507 kb on + strand, within EX28DRAFT_4056 at 30.724 kb on - strand at 30.765 kb on - strand at 30.766 kb on + strand at 30.766 kb on + strand at 30.767 kb on - strand at 30.767 kb on - strand at 30.869 kb on + strand, within EX28DRAFT_4057 at 30.961 kb on + strand, within EX28DRAFT_4057 at 31.065 kb on + strand, within EX28DRAFT_4057 at 31.065 kb on + strand, within EX28DRAFT_4057 at 31.067 kb on + strand, within EX28DRAFT_4057 at 31.068 kb on - strand, within EX28DRAFT_4057 at 31.068 kb on - strand, within EX28DRAFT_4057 at 31.239 kb on - strand, within EX28DRAFT_4057 at 31.319 kb on - strand, within EX28DRAFT_4057 at 31.319 kb on - strand, within EX28DRAFT_4057 at 31.572 kb on + strand at 31.573 kb on - strand at 31.573 kb on - strand at 31.645 kb on + strand at 31.782 kb on + strand at 31.783 kb on - strand at 31.807 kb on + strand at 31.826 kb on + strand at 31.830 kb on - strand at 31.830 kb on - strand at 31.869 kb on + strand, within EX28DRAFT_4058 at 31.870 kb on - strand, within EX28DRAFT_4058 at 31.872 kb on + strand, within EX28DRAFT_4058 at 31.872 kb on + strand, within EX28DRAFT_4058 at 31.873 kb on - strand, within EX28DRAFT_4058 at 31.873 kb on - strand, within EX28DRAFT_4058 at 31.884 kb on - strand, within EX28DRAFT_4058 at 32.093 kb on - strand, within EX28DRAFT_4058 at 32.135 kb on + strand, within EX28DRAFT_4058 at 32.136 kb on - strand, within EX28DRAFT_4058 at 32.262 kb on - strand, within EX28DRAFT_4058 at 32.357 kb on + strand at 32.368 kb on - strand at 32.416 kb on - strand at 32.418 kb on - strand at 32.507 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 29,781 + -2.1 29,936 + EX28DRAFT_4056 0.21 -1.1 30,035 - EX28DRAFT_4056 0.32 +0.3 30,139 + EX28DRAFT_4056 0.43 -0.0 30,139 + EX28DRAFT_4056 0.43 +0.6 30,458 - EX28DRAFT_4056 0.79 -0.1 30,507 + EX28DRAFT_4056 0.84 -0.6 30,724 - +0.1 30,765 - +0.5 30,766 + +0.5 30,766 + -0.1 30,767 - -1.0 30,767 - -1.8 30,869 + EX28DRAFT_4057 0.13 -1.3 30,961 + EX28DRAFT_4057 0.25 -0.3 31,065 + EX28DRAFT_4057 0.39 -0.6 31,065 + EX28DRAFT_4057 0.39 -0.1 31,067 + EX28DRAFT_4057 0.39 +0.6 31,068 - EX28DRAFT_4057 0.39 +0.7 31,068 - EX28DRAFT_4057 0.39 -0.7 31,239 - EX28DRAFT_4057 0.62 -0.6 31,319 - EX28DRAFT_4057 0.72 -0.9 31,319 - EX28DRAFT_4057 0.72 +0.1 31,572 + -0.9 31,573 - +0.5 31,573 - +1.0 31,645 + +0.0 31,782 + -1.1 31,783 - -0.5 31,807 + +0.1 31,826 + +0.7 31,830 - -0.5 31,830 - -0.1 31,869 + EX28DRAFT_4058 0.14 +0.6 31,870 - EX28DRAFT_4058 0.14 +0.9 31,872 + EX28DRAFT_4058 0.14 +1.0 31,872 + EX28DRAFT_4058 0.14 -0.4 31,873 - EX28DRAFT_4058 0.14 -0.3 31,873 - EX28DRAFT_4058 0.14 +0.2 31,884 - EX28DRAFT_4058 0.16 -0.2 32,093 - EX28DRAFT_4058 0.55 +0.1 32,135 + EX28DRAFT_4058 0.63 -1.0 32,136 - EX28DRAFT_4058 0.63 -0.0 32,262 - EX28DRAFT_4058 0.86 +0.5 32,357 + +1.2 32,368 - -0.4 32,416 - -0.8 32,418 - -1.0 32,507 + +0.8
Or see this region's nucleotide sequence