Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3867 and EX28DRAFT_3868 are separated by 15 nucleotides EX28DRAFT_3868 and EX28DRAFT_3869 are separated by 27 nucleotides EX28DRAFT_3869 and EX28DRAFT_3870 are separated by 9 nucleotides
EX28DRAFT_3867: EX28DRAFT_3867 - Glycosyl transferases group 1, at 76,372 to 77,577
_3867
EX28DRAFT_3868: EX28DRAFT_3868 - hypothetical protein, at 77,593 to 78,507
_3868
EX28DRAFT_3869: EX28DRAFT_3869 - L-threonine 3-dehydrogenase (EC 1.1.1.103), at 78,535 to 79,563
_3869
EX28DRAFT_3870: EX28DRAFT_3870 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29), at 79,573 to 80,769
_3870
Position (kb) 78
79
80 Strain fitness (log2 ratio) -2
-1
0
1
2 at 77.539 kb on + strand at 77.539 kb on + strand at 77.540 kb on - strand at 77.588 kb on + strand at 77.589 kb on - strand at 77.589 kb on - strand at 77.589 kb on - strand at 77.589 kb on - strand at 77.592 kb on + strand at 77.592 kb on + strand at 77.592 kb on + strand at 77.592 kb on + strand at 77.592 kb on + strand at 77.592 kb on + strand at 77.593 kb on - strand at 77.593 kb on - strand at 77.593 kb on - strand at 77.593 kb on - strand at 77.599 kb on - strand at 77.625 kb on + strand at 77.626 kb on - strand at 77.695 kb on - strand, within EX28DRAFT_3868 at 77.738 kb on + strand, within EX28DRAFT_3868 at 77.765 kb on - strand, within EX28DRAFT_3868 at 77.881 kb on + strand, within EX28DRAFT_3868 at 77.888 kb on - strand, within EX28DRAFT_3868 at 77.889 kb on + strand, within EX28DRAFT_3868 at 77.890 kb on - strand, within EX28DRAFT_3868 at 77.899 kb on + strand, within EX28DRAFT_3868 at 77.900 kb on - strand, within EX28DRAFT_3868 at 77.955 kb on - strand, within EX28DRAFT_3868 at 77.955 kb on - strand, within EX28DRAFT_3868 at 78.022 kb on + strand, within EX28DRAFT_3868 at 78.023 kb on - strand, within EX28DRAFT_3868 at 78.094 kb on + strand, within EX28DRAFT_3868 at 78.252 kb on - strand, within EX28DRAFT_3868 at 78.285 kb on - strand, within EX28DRAFT_3868 at 78.412 kb on + strand, within EX28DRAFT_3868 at 78.412 kb on + strand, within EX28DRAFT_3868 at 78.412 kb on + strand, within EX28DRAFT_3868 at 78.457 kb on + strand at 78.458 kb on - strand at 78.540 kb on + strand at 78.627 kb on + strand at 78.628 kb on - strand at 78.630 kb on + strand at 78.630 kb on + strand at 78.678 kb on - strand, within EX28DRAFT_3869 at 78.704 kb on + strand, within EX28DRAFT_3869 at 78.704 kb on + strand, within EX28DRAFT_3869 at 78.705 kb on - strand, within EX28DRAFT_3869 at 78.705 kb on - strand, within EX28DRAFT_3869 at 79.149 kb on + strand, within EX28DRAFT_3869 at 79.150 kb on - strand, within EX28DRAFT_3869 at 79.194 kb on - strand, within EX28DRAFT_3869 at 79.236 kb on + strand, within EX28DRAFT_3869 at 79.236 kb on + strand, within EX28DRAFT_3869 at 79.237 kb on - strand, within EX28DRAFT_3869 at 79.254 kb on + strand, within EX28DRAFT_3869 at 79.255 kb on - strand, within EX28DRAFT_3869 at 79.255 kb on - strand, within EX28DRAFT_3869 at 79.294 kb on - strand, within EX28DRAFT_3869 at 79.370 kb on + strand, within EX28DRAFT_3869 at 79.430 kb on + strand, within EX28DRAFT_3869 at 79.430 kb on + strand, within EX28DRAFT_3869 at 79.430 kb on + strand, within EX28DRAFT_3869 at 79.431 kb on - strand, within EX28DRAFT_3869 at 79.582 kb on + strand at 79.719 kb on - strand, within EX28DRAFT_3870 at 79.907 kb on - strand, within EX28DRAFT_3870 at 80.162 kb on + strand, within EX28DRAFT_3870 at 80.163 kb on - strand, within EX28DRAFT_3870 at 80.331 kb on + strand, within EX28DRAFT_3870 at 80.341 kb on + strand, within EX28DRAFT_3870
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 77,539 + +0.0 77,539 + -0.9 77,540 - -1.7 77,588 + -0.2 77,589 - +0.7 77,589 - +1.1 77,589 - +0.5 77,589 - +0.5 77,592 + +0.3 77,592 + +0.2 77,592 + -0.6 77,592 + -0.5 77,592 + -0.1 77,592 + +0.4 77,593 - -0.3 77,593 - +0.8 77,593 - +0.4 77,593 - +0.4 77,599 - -0.1 77,625 + -0.1 77,626 - -2.2 77,695 - EX28DRAFT_3868 0.11 +0.5 77,738 + EX28DRAFT_3868 0.16 +0.5 77,765 - EX28DRAFT_3868 0.19 +0.1 77,881 + EX28DRAFT_3868 0.31 +0.9 77,888 - EX28DRAFT_3868 0.32 +1.8 77,889 + EX28DRAFT_3868 0.32 +0.2 77,890 - EX28DRAFT_3868 0.32 -0.7 77,899 + EX28DRAFT_3868 0.33 +1.0 77,900 - EX28DRAFT_3868 0.34 +0.2 77,955 - EX28DRAFT_3868 0.40 +0.7 77,955 - EX28DRAFT_3868 0.40 +0.2 78,022 + EX28DRAFT_3868 0.47 +2.0 78,023 - EX28DRAFT_3868 0.47 -0.8 78,094 + EX28DRAFT_3868 0.55 -0.7 78,252 - EX28DRAFT_3868 0.72 -0.0 78,285 - EX28DRAFT_3868 0.76 +0.6 78,412 + EX28DRAFT_3868 0.90 +0.3 78,412 + EX28DRAFT_3868 0.90 +1.0 78,412 + EX28DRAFT_3868 0.90 +0.3 78,457 + -0.3 78,458 - -0.2 78,540 + +0.7 78,627 + -0.3 78,628 - -0.2 78,630 + -0.2 78,630 + -0.7 78,678 - EX28DRAFT_3869 0.14 -0.6 78,704 + EX28DRAFT_3869 0.16 -0.7 78,704 + EX28DRAFT_3869 0.16 +0.3 78,705 - EX28DRAFT_3869 0.17 -0.7 78,705 - EX28DRAFT_3869 0.17 -0.5 79,149 + EX28DRAFT_3869 0.60 -1.2 79,150 - EX28DRAFT_3869 0.60 -0.5 79,194 - EX28DRAFT_3869 0.64 -0.9 79,236 + EX28DRAFT_3869 0.68 +0.3 79,236 + EX28DRAFT_3869 0.68 -0.4 79,237 - EX28DRAFT_3869 0.68 +0.9 79,254 + EX28DRAFT_3869 0.70 +0.5 79,255 - EX28DRAFT_3869 0.70 +0.5 79,255 - EX28DRAFT_3869 0.70 +0.1 79,294 - EX28DRAFT_3869 0.74 -0.2 79,370 + EX28DRAFT_3869 0.81 -0.2 79,430 + EX28DRAFT_3869 0.87 +2.4 79,430 + EX28DRAFT_3869 0.87 -0.9 79,430 + EX28DRAFT_3869 0.87 -0.2 79,431 - EX28DRAFT_3869 0.87 -0.5 79,582 + +0.4 79,719 - EX28DRAFT_3870 0.12 -2.5 79,907 - EX28DRAFT_3870 0.28 -0.6 80,162 + EX28DRAFT_3870 0.49 -0.2 80,163 - EX28DRAFT_3870 0.49 +0.3 80,331 + EX28DRAFT_3870 0.63 +0.1 80,341 + EX28DRAFT_3870 0.64 -0.6
Or see this region's nucleotide sequence