Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3799 and EX28DRAFT_3800 are separated by 43 nucleotides EX28DRAFT_3800 and EX28DRAFT_3801 overlap by 4 nucleotides EX28DRAFT_3801 and EX28DRAFT_3802 are separated by 10 nucleotides
EX28DRAFT_3799: EX28DRAFT_3799 - pantothenate kinase (EC 2.7.1.33), at 1,706 to 2,632
_3799
EX28DRAFT_3800: EX28DRAFT_3800 - oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal domain, at 2,676 to 3,689
_3800
EX28DRAFT_3801: EX28DRAFT_3801 - oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal domain, at 3,686 to 4,669
_3801
EX28DRAFT_3802: EX28DRAFT_3802 - ABC-type dipeptide/oligopeptide/nickel transport systems, permease components, at 4,680 to 5,582
_3802
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-1
0
1 at 1.681 kb on + strand at 2.631 kb on - strand at 2.744 kb on - strand at 2.858 kb on + strand, within EX28DRAFT_3800 at 2.861 kb on + strand, within EX28DRAFT_3800 at 2.861 kb on + strand, within EX28DRAFT_3800 at 2.862 kb on - strand, within EX28DRAFT_3800 at 2.983 kb on + strand, within EX28DRAFT_3800 at 2.983 kb on + strand, within EX28DRAFT_3800 at 2.983 kb on + strand, within EX28DRAFT_3800 at 2.984 kb on - strand, within EX28DRAFT_3800 at 2.984 kb on - strand, within EX28DRAFT_3800 at 2.984 kb on - strand, within EX28DRAFT_3800 at 3.008 kb on + strand, within EX28DRAFT_3800 at 3.009 kb on - strand, within EX28DRAFT_3800 at 3.046 kb on + strand, within EX28DRAFT_3800 at 3.046 kb on + strand, within EX28DRAFT_3800 at 3.047 kb on - strand, within EX28DRAFT_3800 at 3.226 kb on + strand, within EX28DRAFT_3800 at 3.226 kb on + strand, within EX28DRAFT_3800 at 3.365 kb on - strand, within EX28DRAFT_3800 at 3.449 kb on - strand, within EX28DRAFT_3800 at 3.449 kb on - strand, within EX28DRAFT_3800 at 3.628 kb on + strand at 3.683 kb on + strand at 3.689 kb on + strand at 3.690 kb on - strand at 3.837 kb on + strand, within EX28DRAFT_3801 at 3.837 kb on + strand, within EX28DRAFT_3801 at 3.838 kb on - strand, within EX28DRAFT_3801 at 3.838 kb on - strand, within EX28DRAFT_3801 at 3.838 kb on - strand, within EX28DRAFT_3801 at 4.058 kb on - strand, within EX28DRAFT_3801 at 4.301 kb on - strand, within EX28DRAFT_3801 at 4.353 kb on + strand, within EX28DRAFT_3801 at 4.459 kb on + strand, within EX28DRAFT_3801 at 4.580 kb on + strand at 4.580 kb on + strand at 4.581 kb on - strand at 4.659 kb on - strand at 4.681 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,681 + -0.3 2,631 - +0.0 2,744 - -0.2 2,858 + EX28DRAFT_3800 0.18 +0.4 2,861 + EX28DRAFT_3800 0.18 -0.5 2,861 + EX28DRAFT_3800 0.18 +0.3 2,862 - EX28DRAFT_3800 0.18 -0.4 2,983 + EX28DRAFT_3800 0.30 +0.2 2,983 + EX28DRAFT_3800 0.30 -0.3 2,983 + EX28DRAFT_3800 0.30 -0.4 2,984 - EX28DRAFT_3800 0.30 +0.1 2,984 - EX28DRAFT_3800 0.30 +1.3 2,984 - EX28DRAFT_3800 0.30 +0.7 3,008 + EX28DRAFT_3800 0.33 +0.4 3,009 - EX28DRAFT_3800 0.33 -0.2 3,046 + EX28DRAFT_3800 0.36 +0.5 3,046 + EX28DRAFT_3800 0.36 +0.7 3,047 - EX28DRAFT_3800 0.37 +0.3 3,226 + EX28DRAFT_3800 0.54 +0.9 3,226 + EX28DRAFT_3800 0.54 -1.1 3,365 - EX28DRAFT_3800 0.68 -0.1 3,449 - EX28DRAFT_3800 0.76 +0.3 3,449 - EX28DRAFT_3800 0.76 +1.3 3,628 + +0.0 3,683 + +1.1 3,689 + +0.4 3,690 - +0.7 3,837 + EX28DRAFT_3801 0.15 -0.1 3,837 + EX28DRAFT_3801 0.15 +0.3 3,838 - EX28DRAFT_3801 0.15 -1.1 3,838 - EX28DRAFT_3801 0.15 +0.3 3,838 - EX28DRAFT_3801 0.15 -1.0 4,058 - EX28DRAFT_3801 0.38 +0.5 4,301 - EX28DRAFT_3801 0.62 +0.2 4,353 + EX28DRAFT_3801 0.68 +0.8 4,459 + EX28DRAFT_3801 0.79 +0.5 4,580 + +0.8 4,580 + +0.3 4,581 - +0.6 4,659 - -0.1 4,681 + +0.1
Or see this region's nucleotide sequence