Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3711 and EX28DRAFT_3712 are separated by 175 nucleotides EX28DRAFT_3712 and EX28DRAFT_3713 are separated by 53 nucleotides EX28DRAFT_3713 and EX28DRAFT_3714 overlap by 38 nucleotides
EX28DRAFT_3711: EX28DRAFT_3711 - pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase, long form, at 382,925 to 384,820
_3711
EX28DRAFT_3712: EX28DRAFT_3712 - dihydrolipoamide dehydrogenase, at 384,996 to 386,420
_3712
EX28DRAFT_3713: EX28DRAFT_3713 - Protein of unknown function (DUF2950), at 386,474 to 387,313
_3713
EX28DRAFT_3714: EX28DRAFT_3714 - Protein of unknown function (DUF3300), at 387,276 to 388,835
_3714
Position (kb) 384
385
386
387 Strain fitness (log2 ratio) -2
-1
0
1 at 384.818 kb on + strand at 386.418 kb on + strand at 386.501 kb on - strand at 386.510 kb on - strand at 386.565 kb on + strand, within EX28DRAFT_3713 at 386.565 kb on + strand, within EX28DRAFT_3713 at 386.566 kb on - strand, within EX28DRAFT_3713 at 386.566 kb on - strand, within EX28DRAFT_3713 at 386.847 kb on + strand, within EX28DRAFT_3713 at 386.848 kb on - strand, within EX28DRAFT_3713 at 386.855 kb on + strand, within EX28DRAFT_3713 at 386.855 kb on + strand, within EX28DRAFT_3713 at 386.856 kb on - strand, within EX28DRAFT_3713 at 386.856 kb on - strand, within EX28DRAFT_3713 at 386.856 kb on - strand, within EX28DRAFT_3713 at 387.256 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 384,818 + -0.7 386,418 + -0.2 386,501 - +0.5 386,510 - +0.6 386,565 + EX28DRAFT_3713 0.11 -0.2 386,565 + EX28DRAFT_3713 0.11 +0.6 386,566 - EX28DRAFT_3713 0.11 +1.3 386,566 - EX28DRAFT_3713 0.11 +0.8 386,847 + EX28DRAFT_3713 0.44 -0.1 386,848 - EX28DRAFT_3713 0.45 +0.3 386,855 + EX28DRAFT_3713 0.45 -0.0 386,855 + EX28DRAFT_3713 0.45 +0.3 386,856 - EX28DRAFT_3713 0.45 -0.2 386,856 - EX28DRAFT_3713 0.45 +1.0 386,856 - EX28DRAFT_3713 0.45 -1.8 387,256 + -0.5
Or see this region's nucleotide sequence