Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3594 and EX28DRAFT_3595 are separated by 39 nucleotides EX28DRAFT_3595 and EX28DRAFT_3596 are separated by 117 nucleotides
EX28DRAFT_3594: EX28DRAFT_3594 - lipoyltransferase and lipoate-protein ligase, at 251,663 to 252,679
_3594
EX28DRAFT_3595: EX28DRAFT_3595 - Uncharacterized membrane protein affecting hemolysin expression, at 252,719 to 253,363
_3595
EX28DRAFT_3596: EX28DRAFT_3596 - phosphoserine phosphatase SerB, at 253,481 to 254,449
_3596
Position (kb) 252
253
254 Strain fitness (log2 ratio) -1
0
1 at 251.762 kb on - strand at 251.899 kb on + strand, within EX28DRAFT_3594 at 251.900 kb on - strand, within EX28DRAFT_3594 at 252.146 kb on - strand, within EX28DRAFT_3594 at 252.146 kb on - strand, within EX28DRAFT_3594 at 252.186 kb on + strand, within EX28DRAFT_3594 at 252.317 kb on - strand, within EX28DRAFT_3594 at 252.419 kb on - strand, within EX28DRAFT_3594 at 252.500 kb on - strand, within EX28DRAFT_3594 at 252.645 kb on + strand at 252.659 kb on - strand at 252.659 kb on - strand at 252.668 kb on + strand at 252.709 kb on + strand at 252.715 kb on - strand at 252.855 kb on - strand, within EX28DRAFT_3595 at 252.883 kb on + strand, within EX28DRAFT_3595 at 252.884 kb on - strand, within EX28DRAFT_3595 at 253.086 kb on + strand, within EX28DRAFT_3595 at 253.298 kb on + strand, within EX28DRAFT_3595 at 253.299 kb on - strand at 253.390 kb on + strand at 253.391 kb on - strand at 253.391 kb on - strand at 253.391 kb on - strand at 253.405 kb on + strand at 253.406 kb on - strand at 253.432 kb on + strand at 253.468 kb on + strand at 253.479 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 251,762 - -1.5 251,899 + EX28DRAFT_3594 0.23 +0.2 251,900 - EX28DRAFT_3594 0.23 +0.2 252,146 - EX28DRAFT_3594 0.47 +0.6 252,146 - EX28DRAFT_3594 0.47 +0.7 252,186 + EX28DRAFT_3594 0.51 +0.8 252,317 - EX28DRAFT_3594 0.64 +0.4 252,419 - EX28DRAFT_3594 0.74 -0.0 252,500 - EX28DRAFT_3594 0.82 +0.2 252,645 + -0.6 252,659 - -0.4 252,659 - -0.6 252,668 + +0.4 252,709 + -1.7 252,715 - -0.1 252,855 - EX28DRAFT_3595 0.21 +0.3 252,883 + EX28DRAFT_3595 0.25 +0.2 252,884 - EX28DRAFT_3595 0.26 +0.1 253,086 + EX28DRAFT_3595 0.57 +0.2 253,298 + EX28DRAFT_3595 0.90 +0.2 253,299 - +0.1 253,390 + +0.1 253,391 - +0.4 253,391 - +0.2 253,391 - -0.3 253,405 + -0.0 253,406 - -0.2 253,432 + +0.0 253,468 + -1.0 253,479 + -0.0
Or see this region's nucleotide sequence