Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3188 and EX28DRAFT_3189 overlap by 11 nucleotides EX28DRAFT_3189 and EX28DRAFT_3190 are separated by 16 nucleotides EX28DRAFT_3190 and EX28DRAFT_3191 overlap by 4 nucleotides
EX28DRAFT_3188: EX28DRAFT_3188 - Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIC, at 488,914 to 489,693
_3188
EX28DRAFT_3189: EX28DRAFT_3189 - Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID, at 489,683 to 490,561
_3189
EX28DRAFT_3190: EX28DRAFT_3190 - Phosphotransferase system, mannose/fructose-specific component IIA, at 490,578 to 491,012
_3190
EX28DRAFT_3191: EX28DRAFT_3191 - N-acetylglucosamine-6-phosphate deacetylase, at 491,009 to 492,142
_3191
Position (kb) 490
491
492 Strain fitness (log2 ratio) -2
-1
0
1
2 at 489.633 kb on + strand at 489.634 kb on - strand at 489.634 kb on - strand at 489.691 kb on + strand at 489.692 kb on - strand at 489.692 kb on - strand at 489.692 kb on - strand at 489.805 kb on - strand, within EX28DRAFT_3189 at 489.859 kb on + strand, within EX28DRAFT_3189 at 489.860 kb on - strand, within EX28DRAFT_3189 at 490.235 kb on + strand, within EX28DRAFT_3189 at 490.349 kb on + strand, within EX28DRAFT_3189 at 490.582 kb on + strand at 490.582 kb on + strand at 490.589 kb on + strand at 490.845 kb on - strand, within EX28DRAFT_3190 at 490.897 kb on + strand, within EX28DRAFT_3190 at 490.897 kb on + strand, within EX28DRAFT_3190 at 490.897 kb on + strand, within EX28DRAFT_3190 at 490.897 kb on + strand, within EX28DRAFT_3190 at 491.074 kb on - strand at 491.074 kb on - strand at 491.159 kb on + strand, within EX28DRAFT_3191 at 491.159 kb on + strand, within EX28DRAFT_3191 at 491.160 kb on - strand, within EX28DRAFT_3191 at 491.243 kb on - strand, within EX28DRAFT_3191 at 491.390 kb on + strand, within EX28DRAFT_3191 at 491.390 kb on + strand, within EX28DRAFT_3191 at 491.467 kb on - strand, within EX28DRAFT_3191 at 491.467 kb on - strand, within EX28DRAFT_3191 at 491.549 kb on - strand, within EX28DRAFT_3191 at 491.549 kb on - strand, within EX28DRAFT_3191 at 491.549 kb on - strand, within EX28DRAFT_3191 at 491.562 kb on + strand, within EX28DRAFT_3191 at 491.648 kb on + strand, within EX28DRAFT_3191 at 491.772 kb on + strand, within EX28DRAFT_3191 at 491.773 kb on - strand, within EX28DRAFT_3191 at 491.833 kb on + strand, within EX28DRAFT_3191 at 491.850 kb on - strand, within EX28DRAFT_3191 at 491.851 kb on + strand, within EX28DRAFT_3191 at 491.852 kb on - strand, within EX28DRAFT_3191 at 491.953 kb on + strand, within EX28DRAFT_3191 at 491.954 kb on - strand, within EX28DRAFT_3191 at 491.954 kb on - strand, within EX28DRAFT_3191
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 489,633 + +2.0 489,634 - -0.0 489,634 - +0.8 489,691 + +0.1 489,692 - +0.6 489,692 - -0.3 489,692 - -0.5 489,805 - EX28DRAFT_3189 0.14 +0.5 489,859 + EX28DRAFT_3189 0.20 +0.9 489,860 - EX28DRAFT_3189 0.20 -0.2 490,235 + EX28DRAFT_3189 0.63 -0.4 490,349 + EX28DRAFT_3189 0.76 -0.4 490,582 + +0.4 490,582 + -0.0 490,589 + -0.0 490,845 - EX28DRAFT_3190 0.61 +2.2 490,897 + EX28DRAFT_3190 0.73 +1.6 490,897 + EX28DRAFT_3190 0.73 +1.4 490,897 + EX28DRAFT_3190 0.73 +0.3 490,897 + EX28DRAFT_3190 0.73 +0.8 491,074 - +0.2 491,074 - +0.4 491,159 + EX28DRAFT_3191 0.13 +1.6 491,159 + EX28DRAFT_3191 0.13 -2.2 491,160 - EX28DRAFT_3191 0.13 +0.8 491,243 - EX28DRAFT_3191 0.21 -0.2 491,390 + EX28DRAFT_3191 0.34 +0.5 491,390 + EX28DRAFT_3191 0.34 +0.5 491,467 - EX28DRAFT_3191 0.40 -0.2 491,467 - EX28DRAFT_3191 0.40 -1.0 491,549 - EX28DRAFT_3191 0.48 +1.2 491,549 - EX28DRAFT_3191 0.48 -0.3 491,549 - EX28DRAFT_3191 0.48 -0.3 491,562 + EX28DRAFT_3191 0.49 -0.1 491,648 + EX28DRAFT_3191 0.56 +0.8 491,772 + EX28DRAFT_3191 0.67 +0.5 491,773 - EX28DRAFT_3191 0.67 -0.3 491,833 + EX28DRAFT_3191 0.73 +0.1 491,850 - EX28DRAFT_3191 0.74 +0.1 491,851 + EX28DRAFT_3191 0.74 -0.7 491,852 - EX28DRAFT_3191 0.74 -0.4 491,953 + EX28DRAFT_3191 0.83 +0.4 491,954 - EX28DRAFT_3191 0.83 -0.1 491,954 - EX28DRAFT_3191 0.83 -0.5
Or see this region's nucleotide sequence