Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3090 and EX28DRAFT_3091 are separated by 145 nucleotides EX28DRAFT_3091 and EX28DRAFT_3092 are separated by 10 nucleotides EX28DRAFT_3092 and EX28DRAFT_3093 are separated by 10 nucleotides EX28DRAFT_3093 and EX28DRAFT_3094 overlap by 8 nucleotides
EX28DRAFT_3090: EX28DRAFT_3090 - Esterase/lipase, at 401,852 to 402,865
_3090
EX28DRAFT_3091: EX28DRAFT_3091 - Urease accessory protein UreH, at 403,011 to 403,838
_3091
EX28DRAFT_3092: EX28DRAFT_3092 - urease, gamma subunit, at 403,849 to 404,151
_3092
EX28DRAFT_3093: EX28DRAFT_3093 - urease, beta subunit, at 404,162 to 404,476
_3093
EX28DRAFT_3094: EX28DRAFT_3094 - urease, alpha subunit, at 404,469 to 406,172
_3094
Position (kb) 403
404 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 402.157 kb on + strand, within EX28DRAFT_3090 at 402.158 kb on - strand, within EX28DRAFT_3090 at 402.301 kb on + strand, within EX28DRAFT_3090 at 402.499 kb on + strand, within EX28DRAFT_3090 at 402.509 kb on + strand, within EX28DRAFT_3090 at 402.510 kb on - strand, within EX28DRAFT_3090 at 402.530 kb on + strand, within EX28DRAFT_3090 at 402.572 kb on + strand, within EX28DRAFT_3090 at 402.573 kb on - strand, within EX28DRAFT_3090 at 402.602 kb on - strand, within EX28DRAFT_3090 at 402.715 kb on + strand, within EX28DRAFT_3090 at 402.715 kb on + strand, within EX28DRAFT_3090 at 402.879 kb on + strand at 402.881 kb on + strand at 402.882 kb on - strand at 402.882 kb on - strand at 402.882 kb on - strand at 402.944 kb on + strand at 402.944 kb on + strand at 403.034 kb on + strand at 403.037 kb on + strand at 403.038 kb on - strand at 403.038 kb on - strand at 403.041 kb on + strand at 403.041 kb on + strand at 403.042 kb on - strand at 403.042 kb on - strand at 403.176 kb on + strand, within EX28DRAFT_3091 at 403.177 kb on - strand, within EX28DRAFT_3091 at 403.528 kb on + strand, within EX28DRAFT_3091 at 403.529 kb on - strand, within EX28DRAFT_3091 at 403.623 kb on + strand, within EX28DRAFT_3091 at 403.624 kb on - strand, within EX28DRAFT_3091 at 403.836 kb on + strand at 403.836 kb on + strand at 403.837 kb on - strand at 403.837 kb on - strand at 403.942 kb on + strand, within EX28DRAFT_3092 at 404.288 kb on + strand, within EX28DRAFT_3093 at 404.289 kb on - strand, within EX28DRAFT_3093 at 404.451 kb on + strand at 404.477 kb on + strand at 404.494 kb on - strand at 404.502 kb on - strand at 404.502 kb on - strand at 404.525 kb on + strand at 404.526 kb on - strand at 404.665 kb on + strand, within EX28DRAFT_3094 at 404.665 kb on + strand, within EX28DRAFT_3094 at 404.665 kb on + strand, within EX28DRAFT_3094 at 404.665 kb on + strand, within EX28DRAFT_3094 at 404.665 kb on + strand, within EX28DRAFT_3094 at 404.666 kb on - strand, within EX28DRAFT_3094
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 402,157 + EX28DRAFT_3090 0.30 -1.3 402,158 - EX28DRAFT_3090 0.30 -0.4 402,301 + EX28DRAFT_3090 0.44 -1.5 402,499 + EX28DRAFT_3090 0.64 +1.0 402,509 + EX28DRAFT_3090 0.65 -1.0 402,510 - EX28DRAFT_3090 0.65 -1.0 402,530 + EX28DRAFT_3090 0.67 -1.1 402,572 + EX28DRAFT_3090 0.71 +3.0 402,573 - EX28DRAFT_3090 0.71 -0.1 402,602 - EX28DRAFT_3090 0.74 -1.5 402,715 + EX28DRAFT_3090 0.85 -0.2 402,715 + EX28DRAFT_3090 0.85 -1.2 402,879 + -0.3 402,881 + -0.3 402,882 - -0.0 402,882 - +0.0 402,882 - +0.4 402,944 + -0.3 402,944 + +0.1 403,034 + -0.2 403,037 + +0.4 403,038 - -0.3 403,038 - -0.3 403,041 + +0.9 403,041 + -0.3 403,042 - -0.4 403,042 - +0.2 403,176 + EX28DRAFT_3091 0.20 -0.8 403,177 - EX28DRAFT_3091 0.20 -0.0 403,528 + EX28DRAFT_3091 0.62 -1.0 403,529 - EX28DRAFT_3091 0.63 +0.0 403,623 + EX28DRAFT_3091 0.74 -1.2 403,624 - EX28DRAFT_3091 0.74 -0.5 403,836 + +0.9 403,836 + -0.2 403,837 - +0.0 403,837 - -1.2 403,942 + EX28DRAFT_3092 0.31 -1.2 404,288 + EX28DRAFT_3093 0.40 -0.1 404,289 - EX28DRAFT_3093 0.40 -0.5 404,451 + -0.6 404,477 + -0.1 404,494 - -0.1 404,502 - -0.2 404,502 - +1.0 404,525 + -0.4 404,526 - -0.1 404,665 + EX28DRAFT_3094 0.12 -0.9 404,665 + EX28DRAFT_3094 0.12 +0.1 404,665 + EX28DRAFT_3094 0.12 +1.0 404,665 + EX28DRAFT_3094 0.12 +0.8 404,665 + EX28DRAFT_3094 0.12 -3.0 404,666 - EX28DRAFT_3094 0.12 +0.1
Or see this region's nucleotide sequence