Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3005 and EX28DRAFT_3006 overlap by 4 nucleotides EX28DRAFT_3006 and EX28DRAFT_3007 are separated by 16 nucleotides EX28DRAFT_3007 and EX28DRAFT_3008 are separated by 13 nucleotides EX28DRAFT_3008 and EX28DRAFT_3009 are separated by 17 nucleotides
EX28DRAFT_3005: EX28DRAFT_3005 - citrate lyase, beta subunit, at 313,482 to 314,357
_3005
EX28DRAFT_3006: EX28DRAFT_3006 - citrate lyase acyl carrier protein, at 314,354 to 314,647
_3006
EX28DRAFT_3007: EX28DRAFT_3007 - [citrate (pro-3S)-lyase] ligase, at 314,664 to 315,686
_3007
EX28DRAFT_3008: EX28DRAFT_3008 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 315,700 to 316,563
_3008
EX28DRAFT_3009: EX28DRAFT_3009 - Na+/citrate symporter, at 316,581 to 317,942
_3009
Position (kb) 314
315
316 Strain fitness (log2 ratio) -1
0
1 at 313.709 kb on + strand, within EX28DRAFT_3005 at 313.709 kb on + strand, within EX28DRAFT_3005 at 313.838 kb on - strand, within EX28DRAFT_3005 at 313.838 kb on - strand, within EX28DRAFT_3005 at 314.050 kb on + strand, within EX28DRAFT_3005 at 314.051 kb on - strand, within EX28DRAFT_3005 at 314.182 kb on + strand, within EX28DRAFT_3005 at 314.183 kb on - strand, within EX28DRAFT_3005 at 314.183 kb on - strand, within EX28DRAFT_3005 at 314.285 kb on - strand at 314.288 kb on + strand at 314.288 kb on + strand at 314.337 kb on - strand at 314.536 kb on + strand, within EX28DRAFT_3006 at 314.665 kb on + strand at 314.789 kb on + strand, within EX28DRAFT_3007 at 314.918 kb on + strand, within EX28DRAFT_3007 at 314.939 kb on + strand, within EX28DRAFT_3007 at 315.011 kb on - strand, within EX28DRAFT_3007 at 315.040 kb on + strand, within EX28DRAFT_3007 at 315.040 kb on + strand, within EX28DRAFT_3007 at 315.040 kb on + strand, within EX28DRAFT_3007 at 315.697 kb on + strand at 315.701 kb on + strand at 315.701 kb on + strand at 315.701 kb on + strand at 315.702 kb on - strand at 315.802 kb on - strand, within EX28DRAFT_3008 at 315.802 kb on - strand, within EX28DRAFT_3008 at 315.802 kb on - strand, within EX28DRAFT_3008 at 315.894 kb on - strand, within EX28DRAFT_3008 at 315.894 kb on - strand, within EX28DRAFT_3008 at 316.104 kb on - strand, within EX28DRAFT_3008 at 316.319 kb on + strand, within EX28DRAFT_3008 at 316.319 kb on + strand, within EX28DRAFT_3008 at 316.319 kb on + strand, within EX28DRAFT_3008 at 316.367 kb on - strand, within EX28DRAFT_3008 at 316.490 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 313,709 + EX28DRAFT_3005 0.26 +0.1 313,709 + EX28DRAFT_3005 0.26 -0.3 313,838 - EX28DRAFT_3005 0.41 -0.2 313,838 - EX28DRAFT_3005 0.41 +0.8 314,050 + EX28DRAFT_3005 0.65 +0.3 314,051 - EX28DRAFT_3005 0.65 +0.5 314,182 + EX28DRAFT_3005 0.80 +0.3 314,183 - EX28DRAFT_3005 0.80 +0.4 314,183 - EX28DRAFT_3005 0.80 -0.5 314,285 - +0.9 314,288 + -0.8 314,288 + +0.2 314,337 - +1.3 314,536 + EX28DRAFT_3006 0.62 +0.1 314,665 + -0.4 314,789 + EX28DRAFT_3007 0.12 -0.2 314,918 + EX28DRAFT_3007 0.25 +0.3 314,939 + EX28DRAFT_3007 0.27 +0.1 315,011 - EX28DRAFT_3007 0.34 -1.2 315,040 + EX28DRAFT_3007 0.37 -0.6 315,040 + EX28DRAFT_3007 0.37 -1.3 315,040 + EX28DRAFT_3007 0.37 +0.4 315,697 + +0.1 315,701 + +0.3 315,701 + +0.0 315,701 + -0.7 315,702 - +0.7 315,802 - EX28DRAFT_3008 0.12 -0.1 315,802 - EX28DRAFT_3008 0.12 -0.1 315,802 - EX28DRAFT_3008 0.12 -0.4 315,894 - EX28DRAFT_3008 0.22 +0.7 315,894 - EX28DRAFT_3008 0.22 -0.7 316,104 - EX28DRAFT_3008 0.47 -0.2 316,319 + EX28DRAFT_3008 0.72 +0.1 316,319 + EX28DRAFT_3008 0.72 +0.1 316,319 + EX28DRAFT_3008 0.72 -0.2 316,367 - EX28DRAFT_3008 0.77 +0.3 316,490 - -0.7
Or see this region's nucleotide sequence