Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2972 and EX28DRAFT_2973 are separated by 49 nucleotides EX28DRAFT_2973 and EX28DRAFT_2974 are separated by 341 nucleotides
EX28DRAFT_2972: EX28DRAFT_2972 - phosphoglycerate kinase (EC 2.7.2.3), at 281,661 to 282,824
_2972
EX28DRAFT_2973: EX28DRAFT_2973 - erythrose-4-phosphate dehydrogenase, at 282,874 to 283,893
_2973
EX28DRAFT_2974: EX28DRAFT_2974 - transketolase, bacterial and yeast, at 284,235 to 286,226
_2974
Position (kb) 282
283
284 Strain fitness (log2 ratio) -2
-1
0
1 at 282.876 kb on - strand at 283.052 kb on - strand, within EX28DRAFT_2973 at 283.070 kb on - strand, within EX28DRAFT_2973 at 283.070 kb on - strand, within EX28DRAFT_2973 at 283.072 kb on - strand, within EX28DRAFT_2973 at 283.313 kb on + strand, within EX28DRAFT_2973 at 283.314 kb on - strand, within EX28DRAFT_2973 at 283.746 kb on - strand, within EX28DRAFT_2973 at 283.832 kb on - strand at 283.833 kb on - strand at 283.848 kb on + strand at 283.848 kb on + strand at 283.849 kb on - strand at 283.893 kb on + strand at 284.079 kb on - strand at 284.087 kb on + strand at 284.088 kb on - strand at 284.117 kb on + strand at 284.172 kb on + strand at 284.173 kb on - strand at 284.173 kb on - strand at 284.173 kb on - strand at 284.173 kb on - strand at 284.173 kb on - strand at 284.226 kb on + strand at 284.227 kb on - strand at 284.236 kb on + strand at 284.237 kb on - strand at 284.362 kb on + strand at 284.363 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 282,876 - +0.2 283,052 - EX28DRAFT_2973 0.17 -0.5 283,070 - EX28DRAFT_2973 0.19 -0.7 283,070 - EX28DRAFT_2973 0.19 -2.3 283,072 - EX28DRAFT_2973 0.19 -0.6 283,313 + EX28DRAFT_2973 0.43 -1.2 283,314 - EX28DRAFT_2973 0.43 -1.2 283,746 - EX28DRAFT_2973 0.85 +0.0 283,832 - -0.5 283,833 - -1.0 283,848 + -1.3 283,848 + -0.2 283,849 - +0.8 283,893 + -0.2 284,079 - +0.2 284,087 + +0.4 284,088 - +0.3 284,117 + -0.3 284,172 + +0.3 284,173 - +1.1 284,173 - +1.0 284,173 - -0.4 284,173 - -0.1 284,173 - +0.1 284,226 + -1.5 284,227 - +0.1 284,236 + -0.4 284,237 - -0.1 284,362 + -1.6 284,363 - +0.8
Or see this region's nucleotide sequence