Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2839 and EX28DRAFT_2840 overlap by 7 nucleotides EX28DRAFT_2840 and EX28DRAFT_2841 overlap by 20 nucleotides EX28DRAFT_2841 and EX28DRAFT_2842 overlap by 4 nucleotides EX28DRAFT_2842 and EX28DRAFT_2843 overlap by 1 nucleotides
EX28DRAFT_2839: EX28DRAFT_2839 - protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 142,060 to 142,686
_2839
EX28DRAFT_2840: EX28DRAFT_2840 - 3'-nucleotidase (EC 3.1.3.6)/5'-nucleotidase (EC 3.1.3.5)/exopolyphosphatase (EC 3.6.1.11), at 142,680 to 143,441
_2840
EX28DRAFT_2841: EX28DRAFT_2841 - tRNA pseudouridine synthase, TruD family, at 143,422 to 144,471
_2841
EX28DRAFT_2842: EX28DRAFT_2842 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 144,468 to 144,947
_2842
EX28DRAFT_2843: EX28DRAFT_2843 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 144,947 to 145,657
_2843
Position (kb)
143
144
145 Strain fitness (log2 ratio)
-1
0
1
2 at 142.502 kb on + strand, within EX28DRAFT_2839 at 142.502 kb on + strand, within EX28DRAFT_2839 at 142.502 kb on + strand, within EX28DRAFT_2839 at 142.503 kb on - strand, within EX28DRAFT_2839 at 142.503 kb on - strand, within EX28DRAFT_2839 at 142.503 kb on - strand, within EX28DRAFT_2839 at 142.503 kb on - strand, within EX28DRAFT_2839 at 142.503 kb on - strand, within EX28DRAFT_2839 at 142.544 kb on - strand, within EX28DRAFT_2839 at 142.580 kb on - strand, within EX28DRAFT_2839 at 142.740 kb on - strand at 142.742 kb on - strand at 142.781 kb on - strand, within EX28DRAFT_2840 at 142.830 kb on - strand, within EX28DRAFT_2840 at 143.134 kb on + strand, within EX28DRAFT_2840 at 143.135 kb on - strand, within EX28DRAFT_2840 at 143.135 kb on - strand, within EX28DRAFT_2840 at 143.316 kb on - strand, within EX28DRAFT_2840 at 143.491 kb on - strand at 143.549 kb on - strand, within EX28DRAFT_2841 at 143.549 kb on - strand, within EX28DRAFT_2841 at 143.791 kb on + strand, within EX28DRAFT_2841 at 143.997 kb on - strand, within EX28DRAFT_2841 at 144.184 kb on - strand, within EX28DRAFT_2841 at 144.232 kb on - strand, within EX28DRAFT_2841 at 144.330 kb on + strand, within EX28DRAFT_2841 at 144.342 kb on + strand, within EX28DRAFT_2841 at 144.436 kb on - strand at 144.447 kb on - strand at 144.447 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 142,502 + EX28DRAFT_2839 0.70 -0.0 142,502 + EX28DRAFT_2839 0.70 +0.3 142,502 + EX28DRAFT_2839 0.70 +0.7 142,503 - EX28DRAFT_2839 0.71 +0.0 142,503 - EX28DRAFT_2839 0.71 -0.7 142,503 - EX28DRAFT_2839 0.71 -0.0 142,503 - EX28DRAFT_2839 0.71 -0.5 142,503 - EX28DRAFT_2839 0.71 -0.5 142,544 - EX28DRAFT_2839 0.77 -0.8 142,580 - EX28DRAFT_2839 0.83 +0.7 142,740 - -0.1 142,742 - -0.8 142,781 - EX28DRAFT_2840 0.13 -0.3 142,830 - EX28DRAFT_2840 0.20 +2.3 143,134 + EX28DRAFT_2840 0.60 -0.8 143,135 - EX28DRAFT_2840 0.60 +0.6 143,135 - EX28DRAFT_2840 0.60 -0.1 143,316 - EX28DRAFT_2840 0.83 -0.8 143,491 - +0.2 143,549 - EX28DRAFT_2841 0.12 -0.4 143,549 - EX28DRAFT_2841 0.12 -0.8 143,791 + EX28DRAFT_2841 0.35 +0.1 143,997 - EX28DRAFT_2841 0.55 -0.2 144,184 - EX28DRAFT_2841 0.73 +0.2 144,232 - EX28DRAFT_2841 0.77 +0.2 144,330 + EX28DRAFT_2841 0.86 -0.4 144,342 + EX28DRAFT_2841 0.88 -0.5 144,436 - +0.7 144,447 - +1.5 144,447 - -0.2
Or see this region's nucleotide sequence