Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2657 and EX28DRAFT_2658 are separated by 107 nucleotides EX28DRAFT_2658 and EX28DRAFT_2659 are separated by 2 nucleotides EX28DRAFT_2659 and EX28DRAFT_2660 are separated by 10 nucleotides EX28DRAFT_2660 and EX28DRAFT_2661 are separated by 6 nucleotides
EX28DRAFT_2657: EX28DRAFT_2657 - Nucleotidyltransferase/DNA polymerase involved in DNA repair, at 2,694,993 to 2,696,051
_2657
EX28DRAFT_2658: EX28DRAFT_2658 - Uncharacterized protein conserved in bacteria, at 2,696,159 to 2,696,359
_2658
EX28DRAFT_2659: EX28DRAFT_2659 - NADH:ubiquinone oxidoreductase, Na(+)-translocating, F subunit, at 2,696,362 to 2,697,585
_2659
EX28DRAFT_2660: EX28DRAFT_2660 - NADH:ubiquinone oxidoreductase, Na(+)-translocating, E subunit, at 2,697,596 to 2,698,192
_2660
EX28DRAFT_2661: EX28DRAFT_2661 - NADH:ubiquinone oxidoreductase, Na(+)-translocating, D subunit, at 2,698,199 to 2,698,837
_2661
Position (kb)
2696
2697
2698 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2695.567 kb on + strand, within EX28DRAFT_2657 at 2695.567 kb on + strand, within EX28DRAFT_2657 at 2695.568 kb on - strand, within EX28DRAFT_2657 at 2695.583 kb on + strand, within EX28DRAFT_2657 at 2695.584 kb on - strand, within EX28DRAFT_2657 at 2695.772 kb on + strand, within EX28DRAFT_2657 at 2695.772 kb on + strand, within EX28DRAFT_2657 at 2695.772 kb on + strand, within EX28DRAFT_2657 at 2695.773 kb on - strand, within EX28DRAFT_2657 at 2695.773 kb on - strand, within EX28DRAFT_2657 at 2695.773 kb on - strand, within EX28DRAFT_2657 at 2695.773 kb on - strand, within EX28DRAFT_2657 at 2695.815 kb on - strand, within EX28DRAFT_2657 at 2695.864 kb on - strand, within EX28DRAFT_2657 at 2696.051 kb on + strand at 2696.055 kb on - strand at 2696.077 kb on - strand at 2696.081 kb on - strand at 2696.083 kb on - strand at 2696.160 kb on + strand at 2696.161 kb on - strand at 2696.165 kb on - strand at 2696.165 kb on - strand at 2696.424 kb on + strand at 2696.460 kb on + strand at 2696.460 kb on + strand at 2696.460 kb on + strand at 2696.460 kb on + strand at 2696.461 kb on - strand at 2696.461 kb on - strand at 2696.461 kb on - strand at 2696.461 kb on - strand at 2696.462 kb on + strand at 2696.462 kb on + strand at 2696.462 kb on + strand at 2696.462 kb on + strand at 2696.462 kb on + strand at 2696.462 kb on + strand at 2696.462 kb on + strand at 2696.463 kb on - strand at 2696.463 kb on - strand at 2696.463 kb on - strand at 2696.463 kb on - strand at 2696.463 kb on - strand at 2696.463 kb on - strand at 2696.634 kb on - strand, within EX28DRAFT_2659 at 2696.634 kb on - strand, within EX28DRAFT_2659 at 2696.679 kb on + strand, within EX28DRAFT_2659 at 2696.679 kb on + strand, within EX28DRAFT_2659 at 2696.679 kb on + strand, within EX28DRAFT_2659 at 2696.846 kb on + strand, within EX28DRAFT_2659 at 2696.846 kb on + strand, within EX28DRAFT_2659 at 2696.847 kb on - strand, within EX28DRAFT_2659 at 2696.986 kb on + strand, within EX28DRAFT_2659 at 2696.987 kb on - strand, within EX28DRAFT_2659 at 2696.987 kb on - strand, within EX28DRAFT_2659 at 2697.053 kb on + strand, within EX28DRAFT_2659 at 2697.147 kb on + strand, within EX28DRAFT_2659 at 2697.150 kb on + strand, within EX28DRAFT_2659 at 2697.151 kb on - strand, within EX28DRAFT_2659 at 2697.165 kb on + strand, within EX28DRAFT_2659 at 2697.171 kb on + strand, within EX28DRAFT_2659 at 2697.341 kb on - strand, within EX28DRAFT_2659 at 2697.475 kb on - strand at 2697.544 kb on + strand at 2697.544 kb on + strand at 2697.597 kb on + strand at 2697.597 kb on + strand at 2697.600 kb on - strand at 2697.622 kb on + strand at 2697.623 kb on - strand at 2697.769 kb on - strand, within EX28DRAFT_2660 at 2697.769 kb on - strand, within EX28DRAFT_2660 at 2697.774 kb on - strand, within EX28DRAFT_2660 at 2697.792 kb on + strand, within EX28DRAFT_2660 at 2697.808 kb on + strand, within EX28DRAFT_2660 at 2697.891 kb on + strand, within EX28DRAFT_2660 at 2697.891 kb on + strand, within EX28DRAFT_2660 at 2697.891 kb on + strand, within EX28DRAFT_2660 at 2697.892 kb on - strand, within EX28DRAFT_2660 at 2697.896 kb on - strand, within EX28DRAFT_2660 at 2698.114 kb on + strand, within EX28DRAFT_2660 at 2698.114 kb on + strand, within EX28DRAFT_2660 at 2698.115 kb on - strand, within EX28DRAFT_2660 at 2698.115 kb on - strand, within EX28DRAFT_2660 at 2698.232 kb on - strand at 2698.468 kb on - strand, within EX28DRAFT_2661 at 2698.468 kb on - strand, within EX28DRAFT_2661 at 2698.546 kb on - strand, within EX28DRAFT_2661
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,695,567 + EX28DRAFT_2657 0.54 -0.2 2,695,567 + EX28DRAFT_2657 0.54 +2.1 2,695,568 - EX28DRAFT_2657 0.54 +0.3 2,695,583 + EX28DRAFT_2657 0.56 +0.4 2,695,584 - EX28DRAFT_2657 0.56 +0.5 2,695,772 + EX28DRAFT_2657 0.74 +0.2 2,695,772 + EX28DRAFT_2657 0.74 +0.2 2,695,772 + EX28DRAFT_2657 0.74 -0.1 2,695,773 - EX28DRAFT_2657 0.74 +0.8 2,695,773 - EX28DRAFT_2657 0.74 +0.2 2,695,773 - EX28DRAFT_2657 0.74 +0.8 2,695,773 - EX28DRAFT_2657 0.74 +0.7 2,695,815 - EX28DRAFT_2657 0.78 +1.0 2,695,864 - EX28DRAFT_2657 0.82 +0.2 2,696,051 + +0.1 2,696,055 - -0.1 2,696,077 - +0.3 2,696,081 - -0.3 2,696,083 - +0.1 2,696,160 + -0.3 2,696,161 - -0.3 2,696,165 - -0.1 2,696,165 - -0.5 2,696,424 + -0.3 2,696,460 + -2.9 2,696,460 + +0.7 2,696,460 + +0.3 2,696,460 + +0.3 2,696,461 - -0.5 2,696,461 - -1.3 2,696,461 - +0.7 2,696,461 - -1.3 2,696,462 + +0.2 2,696,462 + -0.4 2,696,462 + +0.4 2,696,462 + -0.6 2,696,462 + +0.3 2,696,462 + -1.0 2,696,462 + +0.2 2,696,463 - +0.4 2,696,463 - +0.2 2,696,463 - -0.9 2,696,463 - +0.1 2,696,463 - -0.8 2,696,463 - -0.5 2,696,634 - EX28DRAFT_2659 0.22 -0.3 2,696,634 - EX28DRAFT_2659 0.22 -0.7 2,696,679 + EX28DRAFT_2659 0.26 -0.9 2,696,679 + EX28DRAFT_2659 0.26 -0.2 2,696,679 + EX28DRAFT_2659 0.26 -0.6 2,696,846 + EX28DRAFT_2659 0.40 +0.0 2,696,846 + EX28DRAFT_2659 0.40 +0.7 2,696,847 - EX28DRAFT_2659 0.40 -0.1 2,696,986 + EX28DRAFT_2659 0.51 -0.6 2,696,987 - EX28DRAFT_2659 0.51 -0.1 2,696,987 - EX28DRAFT_2659 0.51 -0.3 2,697,053 + EX28DRAFT_2659 0.56 -0.3 2,697,147 + EX28DRAFT_2659 0.64 -0.4 2,697,150 + EX28DRAFT_2659 0.64 -0.3 2,697,151 - EX28DRAFT_2659 0.64 +0.5 2,697,165 + EX28DRAFT_2659 0.66 -0.3 2,697,171 + EX28DRAFT_2659 0.66 +1.5 2,697,341 - EX28DRAFT_2659 0.80 +0.3 2,697,475 - -0.9 2,697,544 + -0.6 2,697,544 + +0.7 2,697,597 + -0.5 2,697,597 + -0.8 2,697,600 - +0.4 2,697,622 + -0.4 2,697,623 - -0.1 2,697,769 - EX28DRAFT_2660 0.29 +0.2 2,697,769 - EX28DRAFT_2660 0.29 +0.8 2,697,774 - EX28DRAFT_2660 0.30 -0.0 2,697,792 + EX28DRAFT_2660 0.33 +0.3 2,697,808 + EX28DRAFT_2660 0.36 -2.9 2,697,891 + EX28DRAFT_2660 0.49 +0.0 2,697,891 + EX28DRAFT_2660 0.49 -1.3 2,697,891 + EX28DRAFT_2660 0.49 -0.2 2,697,892 - EX28DRAFT_2660 0.50 -0.4 2,697,896 - EX28DRAFT_2660 0.50 +0.4 2,698,114 + EX28DRAFT_2660 0.87 -0.2 2,698,114 + EX28DRAFT_2660 0.87 -0.6 2,698,115 - EX28DRAFT_2660 0.87 -0.1 2,698,115 - EX28DRAFT_2660 0.87 +0.1 2,698,232 - -0.7 2,698,468 - EX28DRAFT_2661 0.42 +1.1 2,698,468 - EX28DRAFT_2661 0.42 -0.7 2,698,546 - EX28DRAFT_2661 0.54 +0.7
Or see this region's nucleotide sequence