Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2363 and EX28DRAFT_2364 are separated by 159 nucleotides EX28DRAFT_2364 and EX28DRAFT_2365 are separated by 57 nucleotides EX28DRAFT_2365 and EX28DRAFT_2366 are separated by 183 nucleotides
EX28DRAFT_2363: EX28DRAFT_2363 - ABC-type sugar transport system, ATPase component, at 2,383,569 to 2,385,071
_2363
EX28DRAFT_2364: EX28DRAFT_2364 - Glycerol dehydrogenase and related enzymes, at 2,385,231 to 2,386,319
_2364
EX28DRAFT_2365: EX28DRAFT_2365 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 2,386,377 to 2,387,120
_2365
EX28DRAFT_2366: EX28DRAFT_2366 - Cytotoxic translational repressor of toxin-antitoxin stability system, at 2,387,304 to 2,387,609
_2366
Position (kb)
2385
2386
2387 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2384.329 kb on + strand, within EX28DRAFT_2363 at 2384.478 kb on - strand, within EX28DRAFT_2363 at 2384.505 kb on + strand, within EX28DRAFT_2363 at 2384.879 kb on + strand, within EX28DRAFT_2363 at 2384.880 kb on - strand, within EX28DRAFT_2363 at 2384.899 kb on + strand, within EX28DRAFT_2363 at 2384.973 kb on + strand at 2385.090 kb on - strand at 2385.172 kb on - strand at 2385.193 kb on + strand at 2385.193 kb on + strand at 2385.194 kb on - strand at 2385.214 kb on + strand at 2385.274 kb on - strand at 2385.274 kb on - strand at 2385.274 kb on - strand at 2385.351 kb on + strand, within EX28DRAFT_2364 at 2385.351 kb on + strand, within EX28DRAFT_2364 at 2385.979 kb on - strand, within EX28DRAFT_2364 at 2385.979 kb on - strand, within EX28DRAFT_2364 at 2386.044 kb on + strand, within EX28DRAFT_2364 at 2386.146 kb on - strand, within EX28DRAFT_2364 at 2386.249 kb on - strand at 2386.334 kb on + strand at 2386.334 kb on + strand at 2386.336 kb on + strand at 2386.341 kb on - strand at 2386.341 kb on - strand at 2386.354 kb on - strand at 2386.390 kb on + strand at 2386.390 kb on + strand at 2386.391 kb on - strand at 2386.391 kb on - strand at 2386.398 kb on - strand at 2386.482 kb on - strand, within EX28DRAFT_2365 at 2386.484 kb on + strand, within EX28DRAFT_2365 at 2386.484 kb on + strand, within EX28DRAFT_2365 at 2386.485 kb on - strand, within EX28DRAFT_2365 at 2386.485 kb on - strand, within EX28DRAFT_2365 at 2386.787 kb on + strand, within EX28DRAFT_2365 at 2386.824 kb on - strand, within EX28DRAFT_2365 at 2386.953 kb on - strand, within EX28DRAFT_2365 at 2387.219 kb on + strand at 2387.219 kb on + strand at 2387.252 kb on - strand at 2387.261 kb on - strand at 2387.263 kb on + strand at 2387.264 kb on - strand at 2387.288 kb on + strand at 2387.288 kb on + strand at 2387.300 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,384,329 + EX28DRAFT_2363 0.51 +0.0 2,384,478 - EX28DRAFT_2363 0.60 +1.1 2,384,505 + EX28DRAFT_2363 0.62 -2.2 2,384,879 + EX28DRAFT_2363 0.87 -1.4 2,384,880 - EX28DRAFT_2363 0.87 -0.3 2,384,899 + EX28DRAFT_2363 0.88 +1.8 2,384,973 + -0.7 2,385,090 - +0.2 2,385,172 - -0.7 2,385,193 + +0.1 2,385,193 + -0.4 2,385,194 - +0.6 2,385,214 + +1.1 2,385,274 - +0.3 2,385,274 - +0.8 2,385,274 - -0.7 2,385,351 + EX28DRAFT_2364 0.11 -0.6 2,385,351 + EX28DRAFT_2364 0.11 +0.3 2,385,979 - EX28DRAFT_2364 0.69 +1.0 2,385,979 - EX28DRAFT_2364 0.69 +1.0 2,386,044 + EX28DRAFT_2364 0.75 +0.5 2,386,146 - EX28DRAFT_2364 0.84 -0.5 2,386,249 - +0.2 2,386,334 + -0.2 2,386,334 + -0.8 2,386,336 + +0.4 2,386,341 - -0.3 2,386,341 - +0.2 2,386,354 - -0.4 2,386,390 + -0.2 2,386,390 + -0.3 2,386,391 - +0.2 2,386,391 - -0.0 2,386,398 - -0.0 2,386,482 - EX28DRAFT_2365 0.14 +0.0 2,386,484 + EX28DRAFT_2365 0.14 +0.2 2,386,484 + EX28DRAFT_2365 0.14 +0.3 2,386,485 - EX28DRAFT_2365 0.15 +0.0 2,386,485 - EX28DRAFT_2365 0.15 -0.0 2,386,787 + EX28DRAFT_2365 0.55 +2.8 2,386,824 - EX28DRAFT_2365 0.60 +0.1 2,386,953 - EX28DRAFT_2365 0.77 -0.2 2,387,219 + +1.0 2,387,219 + +0.9 2,387,252 - -0.8 2,387,261 - -1.0 2,387,263 + +0.6 2,387,264 - -0.0 2,387,288 + -1.3 2,387,288 + -0.3 2,387,300 - -0.1
Or see this region's nucleotide sequence