Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2185 and EX28DRAFT_2186 are separated by 168 nucleotides EX28DRAFT_2186 and EX28DRAFT_2187 are separated by 116 nucleotides EX28DRAFT_2187 and EX28DRAFT_2188 are separated by 266 nucleotides EX28DRAFT_2188 and EX28DRAFT_2189 are separated by 68 nucleotides
EX28DRAFT_2185: EX28DRAFT_2185 - transcriptional regulator, TraR/DksA family, at 2,207,728 to 2,207,994
_2185
EX28DRAFT_2186: EX28DRAFT_2186 - Protein of unknown function (DUF1471), at 2,208,163 to 2,208,540
_2186
EX28DRAFT_2187: EX28DRAFT_2187 - Anthranilate phosphoribosyltransferase, at 2,208,657 to 2,209,619
_2187
EX28DRAFT_2188: EX28DRAFT_2188 - P pilus assembly protein, pilin FimA, at 2,209,886 to 2,210,422
_2188
EX28DRAFT_2189: EX28DRAFT_2189 - P pilus assembly protein, chaperone PapD, at 2,210,491 to 2,211,165
_2189
Position (kb)
2208
2209
2210 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2207.959 kb on - strand, within EX28DRAFT_2185 at 2207.987 kb on + strand at 2208.113 kb on + strand at 2208.146 kb on - strand at 2208.221 kb on + strand, within EX28DRAFT_2186 at 2208.275 kb on + strand, within EX28DRAFT_2186 at 2208.296 kb on - strand, within EX28DRAFT_2186 at 2208.296 kb on - strand, within EX28DRAFT_2186 at 2208.363 kb on + strand, within EX28DRAFT_2186 at 2208.399 kb on - strand, within EX28DRAFT_2186 at 2208.487 kb on - strand, within EX28DRAFT_2186 at 2208.533 kb on - strand at 2208.538 kb on + strand at 2208.538 kb on + strand at 2208.801 kb on - strand, within EX28DRAFT_2187 at 2208.801 kb on - strand, within EX28DRAFT_2187 at 2208.801 kb on - strand, within EX28DRAFT_2187 at 2208.804 kb on - strand, within EX28DRAFT_2187 at 2208.969 kb on - strand, within EX28DRAFT_2187 at 2209.154 kb on + strand, within EX28DRAFT_2187 at 2209.156 kb on + strand, within EX28DRAFT_2187 at 2209.157 kb on - strand, within EX28DRAFT_2187 at 2209.209 kb on - strand, within EX28DRAFT_2187 at 2209.358 kb on - strand, within EX28DRAFT_2187 at 2209.424 kb on + strand, within EX28DRAFT_2187 at 2209.424 kb on + strand, within EX28DRAFT_2187 at 2209.432 kb on + strand, within EX28DRAFT_2187 at 2209.433 kb on - strand, within EX28DRAFT_2187 at 2209.535 kb on - strand at 2209.652 kb on - strand at 2209.909 kb on - strand at 2209.976 kb on - strand, within EX28DRAFT_2188 at 2210.188 kb on + strand, within EX28DRAFT_2188 at 2210.277 kb on + strand, within EX28DRAFT_2188 at 2210.277 kb on + strand, within EX28DRAFT_2188 at 2210.277 kb on + strand, within EX28DRAFT_2188 at 2210.278 kb on - strand, within EX28DRAFT_2188 at 2210.299 kb on + strand, within EX28DRAFT_2188 at 2210.299 kb on + strand, within EX28DRAFT_2188 at 2210.405 kb on - strand at 2210.465 kb on - strand at 2210.507 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,207,959 - EX28DRAFT_2185 0.87 -0.1 2,207,987 + -0.6 2,208,113 + +0.1 2,208,146 - -0.2 2,208,221 + EX28DRAFT_2186 0.15 -0.5 2,208,275 + EX28DRAFT_2186 0.30 -0.7 2,208,296 - EX28DRAFT_2186 0.35 +0.2 2,208,296 - EX28DRAFT_2186 0.35 +0.5 2,208,363 + EX28DRAFT_2186 0.53 -2.3 2,208,399 - EX28DRAFT_2186 0.62 -0.4 2,208,487 - EX28DRAFT_2186 0.86 -0.8 2,208,533 - +0.4 2,208,538 + +0.5 2,208,538 + -0.7 2,208,801 - EX28DRAFT_2187 0.15 -1.5 2,208,801 - EX28DRAFT_2187 0.15 -0.2 2,208,801 - EX28DRAFT_2187 0.15 +0.5 2,208,804 - EX28DRAFT_2187 0.15 -0.3 2,208,969 - EX28DRAFT_2187 0.32 -0.2 2,209,154 + EX28DRAFT_2187 0.52 -0.0 2,209,156 + EX28DRAFT_2187 0.52 +0.3 2,209,157 - EX28DRAFT_2187 0.52 +0.1 2,209,209 - EX28DRAFT_2187 0.57 +0.3 2,209,358 - EX28DRAFT_2187 0.73 -1.2 2,209,424 + EX28DRAFT_2187 0.80 +0.0 2,209,424 + EX28DRAFT_2187 0.80 +0.2 2,209,432 + EX28DRAFT_2187 0.80 +0.3 2,209,433 - EX28DRAFT_2187 0.81 -0.5 2,209,535 - +0.1 2,209,652 - -1.3 2,209,909 - -0.7 2,209,976 - EX28DRAFT_2188 0.17 +0.1 2,210,188 + EX28DRAFT_2188 0.56 +1.5 2,210,277 + EX28DRAFT_2188 0.73 -0.2 2,210,277 + EX28DRAFT_2188 0.73 +2.1 2,210,277 + EX28DRAFT_2188 0.73 +1.8 2,210,278 - EX28DRAFT_2188 0.73 -0.1 2,210,299 + EX28DRAFT_2188 0.77 +0.3 2,210,299 + EX28DRAFT_2188 0.77 -0.0 2,210,405 - -0.1 2,210,465 - -0.3 2,210,507 + -0.2
Or see this region's nucleotide sequence